Phytochemical Screening and Antioxidant Activities of Cornukaempferia chayanii Extracts and in vitro Inhibitory Effects on Digestive Enzymes
Keywords:
Antioxidation, Flavonoids, Phenolic compounds, α-glucosidase, α-amylase, Cornukaempferia chayaniiAbstract
Cornukaempferia chayanii is a new species of plant from the genus Cornukaempferia belonging to the family Zingiberaceae. The plant is an endemic species used as a folk medicine in northern and northeastern Thailand. The present study was carried out to conduct phytochemical screening, assess antioxidant activity, and determine the in vitro inhibitory effects of C. chayanii extracts on α-glucosidase and α-amylase enzymes. C. chayanii was collected, processed, and extracted with aqueous (CCA), 50% ethanol (CCHE), and 95% ethanol (CCE). A preliminary phytochemical screening was performed to determine the total phenolic (TPC) and flavonoid (TFC) contents. The antioxidant activities of the extracts were examined by DPPH (2,2-diphenyl-1-picrylhydrazy), ABTS+ (2,2-azinobis-[3-ethylbenzothiazoline-6-sulphonate]) radical scavenging, and FRAP (ferric reducing antioxidant power) assays. The in vitro inhibitory activities of the extracts on α-glucosidase and α-amylase enzymes were also evaluated. The percentage yields of CCA, CCHE, and CCE were 13.22±0.54%, 8.13±0.12%, and 5.02±0.57%, respectively. CCHE had much higher TPC than the CCA and CCE, while TFC was more in the CCE than the other extracts. According to the DPPH and FRAP assays, the CCHE demonstrated significantly higher free radical scavenging activity than the other extracts. Moreover, the CCHE had a similar effect compared to ascorbic acid and Trolox (reference compounds) with the DPPH assay. The CCA had a significant in vitro α-glucosidase and α-amylase enzyme inhibitory effect more than Acarbose, a standard diabetic drug. The findings of this study show that C. chayanii has antioxidant, α-glucosidase, and α-amylase inhibitory properties, and as such, may benefit human health.
References
Assefa ST, Yang EY, Chae SY, Song M, Lee J, Cho MC, Jang S. α Glucosidase inhibitory activities of plants with focus on common vegetables. Plants. 2019; 9(1):2.
Zaynab M, Fatima M, Abbas S, Sharif Y, Umair M, Zafar MH, Bahadar K. Role of secondary metabolites in plant defense against pathogens. Microb Pathog. 2018; 124(4):198-202.
Etsassala NGER, Badmus JA, Waryo TT, Marnewick JL, Cupido CN, Hussein AA, Iwuoha EI. α-glucosidase and α-amylase inhibitory activities of novel abietane diterpenes from Salvia africanalutea. Antioxidants. 2019; 8(10):421.
Liu Y and Shi J. Antioxidative nanomaterials and biomedical applications. Nano Today. 2019; 27(4):146-177.
Prasad S, Gupta SC, Tyagi AK. Reactive oxygen species (ROS) and cancer: role of antioxidative nutraceuticals. Cancer Lett. 2017; 387(1):95-105.
Yang CY, Yen YY, Hung KC, Hsu SW, Lan S, Lin HC. Inhibitory effects of pu-erh tea on α glucosidase and α amylase: a systemic review. Nutr Diabetes. 2019; 9(1):1-6.
Macabeo APG, Pilapil LAE, Garcia KYM, Quimque MT, Phukhamsakda C, Cruz AJC, Hyde KD, Stadler M. α-glucosidase and lipase inhibitory phenalenones from a new species of Pseudolophiostoma originating from Thailand. Molecules. 2020; 25(4):965.
Saensouk S. Endemic and rare plants of ginger family in Thailand. KKU Res J. 2011; 16(3):306-330.
Yupparach P, Wongsuwan P, Phokham B, Sangnark S, Picheansoonthon C. Two new species of Cornukaempferia (Zingiberaceae) from Thailand. J Jap Bot. 2019; 94(2):82-89.
Saensouk P, Theerakulpisut P, Kijwijan B, Bunnag S. Effects of 2,4-D on callus induction from leaf explants of Cornukaempferia larsenii P. Saensouk. Gardens Bulletin Singapore. 2007; 59(1&2):183-188.
Chanhan P, Konsue A, Nammatra R. Effect of agricultural model of using fertilizer, harvesting time and extraction method on hytochemical contents and antioxidant activities from mulberry leaves grown in Maha Sarakham Province, Thailand. Pharmacog J. 2019; 11(3):531-535.
Taepongsorat L and Konsue A. Biological screening of trijannarose as a recipe from Thai traditional medicine. Phcog Res. 2019; 11(2):110-114.
Puangpornpitag D, Tankitijano P, Sumalee A, Konsue A. Phytochemical screening and antioxidant activities of the seedling extracts from Inca Peanut Plukenetia volubilis. Pharmacog J. 2021; 13(1):1-7.
Mitra BDA and Manjunatha M. Studies on the anti-diabetic and hypolipidemic potentials of mangiferin (Xanthone Glucoside) in streptozotocin-induced Type 1 and Type 2 diabetic model rats. Int J Adv Pharm Sci. 2010; 1(1):75-85.
Umeno A, Horie M, Murotomi K, Nakajima Y, Yoshida Y.Antioxidative and antidiabetic effects of natural polyphenols and isoflavones. Molecules. 2016; 21(708):1-15.
Kamsu GT, Tagne SR, Fodouop CS, Ndel Famen LC, Kodjio N, Endeguele ES, Gatsing D. In vitro antisalmonellal and antioxidant activities of leaves extracts of Tectona grandis L. F. (Verbenaceae). Eur J Med Plant. 2019; 29(4):1-13.
Andriani Y, Mohamad H, Kassim MI, Rosnan ND, Syamsumir DF, Saidin J, Muhammad TST, Amir H. Evaluation on Hydnophytum formicarum tuber from Setiu Wetland (Malaysia) and Muara Rupit (Indonesia) for antibacterial and antioxidant activities, and anti-cancer
potency against MCF-7 and HeLa cells. J Appl Pharm Sci. 2017; 7 (9):30-37.
Liu F, Ma H, Wang G, Liu W, Seeram NP, Mu Y, Xu Y, Huang X, Li L. Phenolics from Eugenia jambolana seeds with advanced glycation end product formation and α-glucosidase inhibitory activities. Food Funct. 2018; 9(8):4246-4254.
Da Costa Pinaffi AC, Sampaio GR, Soares MJ, Shahidi F, De Camargo AC, Torres EAFS. Insoluble-bound polyphenols released from guarana powder: inhibition of α-glucosidase and proanthocyanidin profile. Molecules. 2020; 25(3):679.
De Camargo AC, Regitano-d’Arce MAB, Shahidi F. Phenolic profile of peanut by-products: antioxidant potential and inhibition of α-glucosidase and lipase activities. J Am Oil Chem Soc. 2017; 94(7):959-971.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
