Effect of Methanol Extract of Curcuma longa L on some Biochemical and Haematological Parameters in Albino Rats
doi.org/10.26538/tjnpr/v4i11.30
Keywords:
Plant extraction, Toxicology, Methanol extract, Curcuma longaAbstract
C. longa (Turmeric) is a plant that is used both as spice and medicine. Despite all the documented importance of turmeric in both nutrition and medicine, there is little or no scientific evidence on the biochemical effect of the methanol extract in albino rats. This study was aimed at evaluating the effect of the methanol extract on some selected biochemical parameters in albino rats. In
the assessment of the 15-day sub-acute toxicity profile of the methanol extract of C. longa, acuteand sub-acute toxicity study was performed following standard laboratory procedures using twenty albino rats which were divided into four groups of 5 rats each. The rats were administered different concentrations of the methanol extracts, 100 mg/kg b.wt, 200 mg/kg b.wt and 500mg/kg b.wt whereas control received distilled water only. At the end of each period of administration, some biochemical parameters and haematological parameters were analysed. There was a significant increase (p<0.05) in parameters such as High-Density Lipoprotein cholesterol (HDL-c), malondialdehyde, haemoglobin, Glutathione reductase (GHSR), red blood cell count, and packed cell volume in rats administered the methanol extract. In contrast, a significant decrease (p < 0.05) was also observed in parameters such as Catalase (CAT), Superoxide dismutase (SOD), and blood glucose level. The result has shown that the methanol extract of turmeric has blood glucose and blood lipid-lowering potentials, and as well possesses hematopoietic potentials.
References
Prabhakaran NKP. The Agronomy and Economy of Turmeric and Ginger: The Invaluable Medicinal Spice Crops. Elsevier USA. 2013. 544 p.
Odigie EB, Airiagbonbu BO, Airiagbonbu NT, Omorodion NT. Respiratory Tract Protective Herbs May Serve As Palliatives in the Battle against COVID-19 Pandemic. Int J Basic SciTech. 2020; 6(2):65-75.
Chattopadhyay I, Biswas K, Bandyopadhyay U, Banerjee RK. Turmeric and Curcumin: biological actions and medicinal applications. Curr Sci. 2014; 87(1):44-53.
Verma RK, Kumari P, Maurya RK, Kumar V, Verma RB, Singh RK. Medicinal properties of turmeric (Curcuma longa L.): A review. IntJ Chem Stud. 2018; 6(4):1354-1357.
Nasri H, Sahinfard N, Rafieian M,Rafieian S, Rafieian M, Shirzad F. Turmeric: A spice with multifunctional medicinal properties. HerbmedPharmacol. 2014; 3(1):5-8.
Sarangthem K and Haokip MJ. The bioactive component in Curcuma caesiaRoxb grown in Manipur. The Bioscan,2010; 5:113-115.
Lorke D. A new approach to practical acute toxicity testing.ArchToxicol.1983;53:275-289.
Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. Enzymatic determination of total cholesterol. ClinChem.1976;20(4):470-475.
Albers JJ, Cheung MC, Hazzard WR. High-density lipoprotein in myocardial infarction survivors. Metab. 1978; 27:479.
Wallin B, Rosengren B, Shertzer HG, Camejo G. Lipoprotein oxidation and measurement of thiobarbituric acid reacting substances formation in a single microtitre plate: its use for evaluation of antioxidants. AnalBiochem.1993;208:10-15.
Yogashi Y, Shirakawa J, Okuyama T, Yamazaki S, Kyohara M, Miyazawa A, Suzuki T, Hamada M, Terauchi Y. Evaluation of the appropriateness of using glucometers for measuring the blood glucose levels in mice. SciRep. 2016;6:25465.
Ochei JO and Kolhatkar AA. Medical Laboratory Science:Theory and Practice, Tata McGraw-Hill Publication Company limited, New York. 2008. 637-745 p.
Kirk RS and Sawyer R. Pearson’s composition and analysis of foods. Church hill, Livingstone, Edinburgh, 1998. 615-616.
El-Olemyl MM, Al-Muhtadi FJ, Afifi AA. Experimental Phytochemistry. A laboratory manual (King Saud University, KSA). 1994. 8-9 p.
Aebi HE. Catalase. In: Bergmeyer Hu (ed), Methods of enzymatic analysis, VerlaChemie, Weinnheim, Germany,1983; 273-285.
Fridovich I. Superoxide dismutase. An adaptation to a paramagnetic gas. J Biol Chem. 1989; 264:7761-7764.
Habig WH, Pabst MJ, Jacoby WBC. Glutathione Stransferases: the first enzymatic step in mercapturic acid formation. J Biol Chem. 1974; 249:7130-7139.
Piero NM, Eliud NN, Susan KN, George OO, David NJMM. In vivoAntidiabetic Activity and Safety in rats of cissampelospareiratraditionally used in the management of diabetes mellitus in Embu County, Kenya. J Drug MetabToxicol. 2015; 6:184.
Muriithi NJ, Maina GS, Maina MB, Klambi MJ, Kelvin JK. Determination of the haematological effect of methanolic leaf extract of Vernonialasiopusin normal mice. J Blood Lymph. 2015; 5:139.
Sanchez-Ramon S, Lopez-Longo FJ, Carreno L. Interleukins network in rheumatoid arthritis pathophysiology: beyond proinflammatory cytokines. RheumatolClin. 2011; 6:20-24.
Yang Y, Sterling J, Storici F, Resnick MA, Gordenin DA. Hypermutability of damaged single-strand DNA formed at double-strand breaks and uncapped telomeres in yeast Saccharomyces cerevisiae. PLoSGenetics. 2008; 4(11):e1000264.
Ngugi MP, Njue ME, Gathumbi K. P.Trace elements content of selected Kenyan antidiabetic Medicinal Plants.Int J Curr Pharm Res. 2012; 4(3):39-42.
Kumari M and Jain S. Tannins: An antinutrient with a positive effect to manage diabetes. Res J Recent Sci. 2012; 1(12):1-18.
Aggarwal BB, Ichikawa H, Garodia P. From Traditional Ayurvedic Medicine to Modern Medicine: Identification of Therapeutic Targets for Suppression of Inflammation and Cancer. ExpertOpinTher Targets. 2006; 10:87-118.
Mujoo K, Haridas V, Hoffmann JJ, Wächter GA, Hutter LK, Lu Y, Blake ME, Jayatilake GS, Baile D, Mills GB, Gutterman JU.Triterpenoid saponins from Acacia victoriae (Bentham) decrease tumour cell proliferation and induce apoptosis. Cancer Res. 2001; 61(14):5486-5490.
Grace G, Bomkazi MG, Collise N, Nomalungelo IG. The proximate composition of S. Nigrum plant leaves consumed in the Eastern Cape Province of South Africa.Int Pro ChemBiol Environ Eng. 2013; 53:103-106.
Dholi SK, Raparla R, Mankala SK, Nagappan K. In vivo antidiabetic evaluation of neem leaf in Alloxan-induced rats. J Appl Pharm Sci. 2011; 1(4):100-105.
Ros SJ, Cabiscol EC, Tamarit SJ. Oxidative stress in bacteria and protein damage by reactive oxygen species. Int. JMicrobiol. 2000; 3(1):3-8.
Liu Z, Solter P, Guzman R. Decreased Hepatic ALT Synthesis is an Outcome of Subchronic Microcystin-LR Toxicit . ToxicolApplPharmacol. 2000; 164:216-220.
Patel MD and Thompson PD. Phytosterols and vascular disease. Atheroscler. 2006, 186(1):12-19.
Ambrosio CLB, de Arruda CF, Siqueira C, de Faro ZP. Carotenoides Como Alternativa contra hipovitaminose A. Revista de Nutricao. 2016; 9(2):233-243.
Disilvestro RA, Joseph E, Zhao S, Bomser J. Diverse effect of low dose supplement of lapidated Curcumin in healthy middle-aged people. NutrJ. 2012; 11(1):79.
Madaric A, Kadrabova J, Krajcovicova-Kudlackova M, Valachovicova M, Spustova V, Mislanova C, Kajaba I, Blazicek P. The effect of a bioactive complex of quercetin, selenium, catechins, and curcumin on cardiovascular risk markers in healthy population after two months consumption. Bratislavskélekárskelisty - Bratislava Med J. 2013;114:84-87.
Mohammadi MT, Jahromi MG, Mirjalili MH, Binabaj M R, Jafari M, Salem F. Atorvastatin inhibits the brain oxidative stress of streptozotocin-induced diabetic rat. J ExpApplAnimSci. 2013; 1(1):35-43.
Panahi Y, Rahimnia A, Sharafi M, Alishiri G, Saburi A, Sahebkar A. Curcuminoid treatment for knee osteoarthritis: a randomized double-blind placebo-controlled trial. Phytother Res. 2014; 28:1625-1631.
Sanmukhani J, Satodia V, Trivedi J, Patel T, Tiwari D, Panchal B, Goel A, Tripathi CB. Efficacy and safety of curcumin in major depressive disorder: a randomized trial. Phytother Res. 2014; 23(4):579-585.
Botella-Pavia P and Rodriguez-Concepcion M. Carotenoid biotechnology in plants for nutritionally improved foods. Physiol Plant. 2006; 126(3):369-381.
Vance DE and Vance JE. Biochemistry of lipids, lipoproteins and membranes. Elsevier Amsterdam. 2012. 15-21 p.
Masuda T, Maekawa T, Hidaka K, Bando H, Takeda Y, Yamaguchi H. Chemical studies on antioxidant mechanisms of Curcumin: analysis of oxidative coupling products from curcumin and linoleate. J AgricFood Chem. 2011; 49:2539- 2547.
Chan K, Shien J, Tiley L, Chen D, Chiou S, Wang S, Chang T, Lee Y, Hsu W. Curcumin inhibits influenza virus infection and haemagglutination activity. Food Chem. 2010; 119(4):1346-1351.
Ukoha OA and Onunkwo DN. The effect of turmeric (Curcuma longa) on the haematology and blood chemistry of broiler chickens. Sci-Afri J Sci Issues Res Essays. 2016; 4(6):964-968.
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