Protective Roles of Annona senegalensis Pers. Extract in N-DiethylnitrosamineInduced Hepatocellular Carcinoma in Male Wistar Rats
doi.org/10.26538/tjnpr/v4i11.23
Keywords:
Gene expression, Hepatocellular carcinoma, Annona senegalensis, N-diethylnitrosamineAbstract
Hepatic cancer is a common and leading cancer around the world. Preventive measures have been emphasized in view of its prognosis and limited treatment. This study evaluates the protective roles of Annona senegalensis in N-diethylnitrosamine (DEN) – a model of
hepatocarcinoma in male Wistar rats. Rats were intra-peritoneally induced weekly with 100 mg/kg of DEN for 3 weeks and treated with Annona senegalensis extract using a cannula for 21 days. Biochemical assays; aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lipid peroxidation were carried out. Reverse transcription-polymerase chain reactions for the expression of pro-inflammatory RNA, interleukin 6 (IL-6), were equally determined. Annona senegalensis extract (100 mg/kg and 200 mg/kg body weight) significantly (p < 0.05) reduced the activities of ALT and AST in DEN-induced hepatocarcinoma. Lipid peroxidation associated
with the DEN model of the hepatocarcinoma was reversed, and the expression of the proinflammatory, IL-6 mRNA was also down-regulated with Annona senegalensis extract. Histopathological analysis revealed that the treatment (Annona senegalensis extract) restored the liver architecture in DEN model of hepatocarcinoma. Restoration of the liver enzymes, downregulation of the pro-inflammatory, IL-6 mRNA, and restoration of the liver architecture in the DEN model of hepatocarcinoma corroborated the protective potentials of Annona senegalensis extract against hepatocarcinoma.
References
Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics CA Cancer. J Clin. 2011; 61(2):69- 90.
Kumar V, Kato N, Urabe Y, Takahashi A, Muroyama R, Hosono N, Koike K. Genome-wide association study identifies a susceptibility locus for HCV-induced hepatocellular carcinoma. Nature Gene. 2011; 43:455-460.
El-Serag HB and Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterol. 2007; 132(7):2557-2576.
Ding YF, Peng ZX, Ding L, Peng YR. Baishouwu Extract Suppresses the Development of Hepatocellular Carcinoma via TLR4/MyD88/ NF-_B Pathway Front Pharmacol. 2019; 10:389.
Mustapha A, Owuna G, Uthman I. Plant Remedies Practiced by Keffi People in the Management of Dermatosis. J Med Pl Stu. 2013; 1(5):112-118.
Aiyeloja A and Bello O. Ethnobotanical Potentials of Common Herbs in Nigeria: A Case Study of Enugu State. Edu Res Rev. 2006; 1(1):16-22.
Dambatta S and Aliyu B. A Survey of Major Ethno medicinal plants of Kano North, Nigeria, their Knowledge and Uses by Traditional Healers. Bayero J Pure Appl Sci. 2011; 4(2):28-34.
Noumi E and Safiatou M. Some Investigations on the Traditional Pharmacopoeia about Venomous Bites and Stings from Scorpions, Snakes and Spiders in the Hina Subdivision, Far North, Cameroon, Brit J Pharm Res. 2015; 5(5):344-358.
Jiofack T, Fokunang C, Guedje N, Kemeuze V, Fongnzossie E, Nkongmeneck BA, Tsabang N. Ethnobotanical uses of some plants of two ethnoecological regions of Cameroon. Afr J Pharm Pharmacol. 2009; 3(13):664-684.
National Institute of Health (NIH). Guide for the Care and Use of Laboratory Animals, USA: NIH Publication. 2011. 85-123 p.
Sundaresan S and Subramanian P. S-allylcysteine inhibits circulatory lipid peroxidation and promotes antioxidants in N-nitrosodiethylamine-induced carcinogenesis. Pol J Pharmacol. 2003; 55(1):37-42.
Rotimi SO, Adelani IB, Bankole GE, Rotimi OA. Naringin enhances reverse cholesterol transport in high fat/low streptozocin induced diabetic rats. Biomed Pharmacother. 2018; 101:430-437.
Buege JA and Aust SD. Microsomal lipid peroxidation Meth Enzymol. 1978; 52:302-310.
Aliyu R, Adebayo AH, Gatsing D, Garba H. The effects of ethanolic leaf extract of Commiphora africana (Burseraceae) on rat liver and kidney functions. J Pharmacol Toxicol. 1998; 2:373-379.
Osifo UC, Akpamu U, Idehen IC, Adisa AW, Azeke EK. The effect of chronic ingestion of crude Garcinia kola on the histology of the liver. Eur J Exp Biol. 2012; 2:404-409.
Mittal G, Brar APS, Soni G. Impact of hypercholesterolemia on toxicity of N-nitrosodiethylamine: biochemical and histopathological effects. Pharmacol Rep. 2006; 58(3):413-419.
Arboatti AS, Lambertucci F, Sedlmeier MG, Pisani G, Monti J, Álvarez MDL, Carnovale CE. Diethylnitrosamine increases proliferation in early stages of hepatic carcinogenesis in insulin-treated type 1 diabetic mice. BioMed Res Int. 2018; 2018:1-10.
Qiu W, Wang X, Leibowitz B, Yang W, Zhang L, Yu J. PUMA–mediated apoptosis drives chemical hepatocarcinogenesis in mice. Hepatology. 2011; 54(4):1249-1258.
Sayed-Ahmed MM, Aleisa AM, Al-Rejaie S S, Al-Yahya AA, Al-Shabanah OA, Hafez MM, Nagi MN. Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling. Oxida Med Cellul Longev. 2010; 3(4):254-261.
Cardin R, Piciocchi M, Bortolami M, Kotsafti A, Barzon L, Lavezzo E, Sinigaglia A, Rodriguez-Castro KI, Rugge M, Farinati F. Oxidative damage in the progression of chronic liver disease to hepatocellular carcinoma: An intricate pathway. World J Gastroenterol. 2014; 20:3078-3086.
Sehrawat A and Sultana S. Evaluation of possible mechanisms of protective role of Tamarix gallica against DEN initiated and 2-AAF promoted hepatocarcinogenesis in male Wistar rats. Life Sci. 2006; 79:1456-1465.
Sallie R, Tredger JM, William R. Drugs and the Liver. Biopharm Drug Dispos. 1991; 12:251-259.
Sayeed I, Parvez S, Pandey S, Bin-Hafeez B, Haque R, Raisuddin S. Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus Bloch. Ecotoxicol Environ Saf. 2003; 56:295-301.
Tan BL, Norhaizan ME, Liew WPP, Sulaiman Rahman H. Antioxidant and oxidative stress: a mutual interplay in agerelated diseases. Frontiers in pharmacology. 2018; 9:1162.
Yang H and Dou QP. Targeting apoptosis pathway with natural terpenoids: implications for treatment of breast and prostate cancer. Curr Drug Targets 2010; 11(6):733-744.
Tanaka T, Narazaki M, Kishimoto T. IL-6 in inflammation, immunity, and disease. Cold Spring Harbor Perspec Biol. 2014; 6(10):a016295.
Li CM, Deng J, Hu X, Li L, Chen Y, Ju J, Hao S. Chronic inflammation contributes to the development of hepatocellular carcinoma by decreasing miR-122 levels. Oncotarget. 2016; 7(13):17021–17034.
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