Antifungal Activity of Telfaira occidentalis Extracts on some Phytopathogenic Fungi Isolated from Carica papaya
doi.org/10.26538/tjnpr/v4i11.22
Keywords:
Antifungal, Telfaira occidentalis, Fungi, Carica papaya, PhytochemicalsAbstract
Infused within plants are potent phytochemicals with beneficial activities to mankind which includes the management and treatment of microbial infections. Fungal diseases result in significant economic losses and hindrance of global papaya production. Phytochemicals have made medicinal plants become sources of environmentally friendly alternative antimicrobials. This study aimed at evaluating the antifungal activity of leaf extracts of Telfeira occidentalis against phytopathogenic fungi isolated from Carica papaya. Ethyl acetate, n-Hexane, ethanol, methanol and aqueous extracts of Telfeira occidentalis leaves were evaluated for their antimicrobial properties. Agar-well diffusion method was employed for in vitro screening, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the extract types against test fungal species of Aspergillus, Penicillium, Rhizopus and Trichoderma.
All the extracts evaluated inhibited fungal growth to some degree, with the aqueous extract exhibiting more inhibitory activities than the organic extracts. MIC and MFC values of the extracts ranged between 15.625 to 31.25 and 62.5 to 125 mg/mL, respectively. The antifungal activity of T. occidentalis was found to be equal or higher than commercially available fungicide, ketoconazole. The results of this study indicate that leaf extracts of T. occidentalis has potential for use as bio-fungicides for plant protection against fungal diseases.
References
Ahvazi M, Khalighi-Sigaroodib F, Charkhchiyanc MM, Mojabd F, Mozaffariane V-A, Zakerif H. Introduction of medicinal plants species with the most traditional usage in Alamut region. Iranian J Pharm Res. 2012; 11(1):185-194.
Abu-Darnish MS and Efferth T. Medicinal plants from near East for cancer therapy. Front Pharmacol. 2018; 9(56):1-17.
Mahesh B and Satish, S. Antimicrobial activity of some important medicinal plant against plant and human pathogens. World J Agric Sci. 2008; 4:839-843.
Al-Taisan WA, Bahkali AH, Elgorban AM, El-Metwally MA. Effective influence of essential oils and microelements against Sclerotinia sclerotiorum. Int J Pharmacol. 2014; 10:275-281.
Al-Muniri RMS and Hossain MA. Evaluation of antioxidant and cytotoxic activities of different extracts of folk medicinal plant Hapllophyllum tuberculatum. Egypt J Basic Appl Sci. 2017; 4:101-106.
Kooti W and Daraei N. A review of the antioxidant activity of celery (Apium graveoleus L.). J Evid-Based Compl Altern Med. 2017; 22(4):1029-1034.
Ates DA and Erdogrul OT. Antimicrobial activities of various medicinal and commercial plant extracts. Turk J Biol. 2003; 27:157-162.
Zare-Marzouni H, Daraei N, Sharafi-Ahvazi N, Kalani N, Kooti W. The effects of aqueous extract of celery leaves (Apium graveveoleus) on fertility in female rats. World J Pharm Pharm Sci. 2016; 5:1710-1714.
Anyanwu MU and Okoye RC. Antimicrobial activity of Nigerian medicinal plants. J Intercult Ethnopharmacol. 2017; 6(2):240-259.
Elgorban AM, Bahkali AH, El- Metwally MA, Elsheshtawi M, Abdel- Wahab MA. In vitro antifungal activity of some plant essential oils. Inter J Pharmacol. 2015; 11:56-61.
Ramezani H, Singh HP, Batish DR Kohli, Dargan JS. Fungicidal effect of volatile oils from Eucalyptus citriodora and its major constituent citronetial. New Zealand Plant Protect. 2002; 55:327-330.
Nakamura CV, Ishida K, Faccin LC, Filho BPD, Cortez DAG, Rozental S, de Souza W, Ueda-Nakamura T. In vitro activity of essential oil from Ocimum gratissimum L. against four Candida species. Res Microbiol. 2004; 155:579-586.
Senguttuvan J, Paulsamy S, Krishnamoorthy K. In vitro antifungal activity of leaf and root extracts of the medicinal plant, Hypochaeris radicata L. Inter J Pharm Pharm Sci. 2013; 5(3):758-761.
Shuping DSS and Eloff JN. The use of plants to protect plants and food against fungal pathogens: A review. Afr J Trad Compl Altern Med. 2017; 14(4):120-127.
Patel R, Pandya J, Purohit N. Antifungal activity of Tithonia rotundifolia Blake and Tagetes erecta L. against Aspergillus niger. Int J Creat Res Thou. 2020; 8(7):2320- 2882.
Agrios GN. Losses caused by plant diseases. Plant Patho. United Kingdom Elsevier, Oxford; 2004. 45 p.
Dellavalle PD, Cabrera A, Alem D, Larrañaga P, Ferreira F, Dalla Rizza M. Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chilean J Agric Res. 2011; 71(2):231-239.
Joshi S, Kumar P, Pant P, Sati SC. A comparative evaluation of Kumaun himalayan gymnosperms for their antifungal potential against plant pathogenic fungi. The J Phytopharmacol. 2018; 7(3):230-241.
Rai MK and Carpinella C. Naturally occurring bioactive compounds: a new and safe alternative for control of pests and microbial diseases (3rd ed.). Netherlands: Elsevier Pub; 2006. 514 p.
Kagale S, Marimuthu T, Thayumanavan B, Nandakumar R, Samiyappan R. Antimicrobial activity and induction of systemic resistance in rice by leaf extract of Datura metel against Rhizoctonia solani and Xanthomonas oryzae pv. oryzae. Physio and Mol Plant Pathol. 2004; 65:91-100.
Milan P, Hassler SC, Baysal-Gurel F. Methods for management of soilborne diseases in crop production. Agric. 2020; 10(16):1-21.
Wisplinghoff H, Bischoff T, Tallent SM, Seifert H, Wenzel RP, Edmond MB. Nosocomial bloodstream infections in US hospitals: analysis of
,179 cases from a prospective nationwide surveillance study. Clin Infect Dis. 2004; 39:309-317.
Venkatesan P, Perfect JR, Myers S. Evaluation and management of fungal infections in immune compromised patients. Dermatol Ther. 2005; 18:44-57.
Kayode AAA and Kayode OT. Some medicinal values ofTelfairia occidentalis: A Review. Am J Biochem Mol Biol. 2011; 1:30-38.
Opara EU and Uduak R. Effect of agro effluents on the control of bacterial spot disease of fluted pumpkin (Telfaira occidentalis Hook f.) in South East Nigeria. Nigerian Agric J. 2019; 50(2):123-129.
Orole RT, Orole OO, Aisoni JE, Isyaku J, Mohammed YS. Comparative study of the physicochemical properties of male and female flutted pumpkin (Telfaira occidentalis). The J Med Res. 2020; 6(2):55-61.
Okoye EI and Orakwue FC. The chemical evaluation and anti-microbial screening of extracts from seeds and leaves of Telfairia occidentalis (Fluted pumpkin). Chem Res J. 2019; 4(3):98-104.
Bassey IN and Opara EU. Potency of plant ashes as organic fertilizers in the performance and control of leaf spot disease of Telfairia occidentalis in South Eastern Nigeria. J Agric Sustain. 2016; 9(2):210-227.
Umeoka N. Antifungal effect and phytochemical screening of Telfeira occidentalis (hook f.) leaf extracts. J Plant Biotech Microbiol. 2018; 1(1):21-24.
Olaniyi JO and Oyerele TA. Growth, nield and Nutritional compositions of fluted pumpkin (Telfairia occidentalis) as affected by fertilizer types in Ogbomoso, Southwest Nigeria. Bull Environ Pharmacol Life Sci. 2012; 1(9):81- 88.
Osai EO, Udo SE, Okoli CE, Bemoh BE. Comparative efficacy of three plant extracts for the control of leaf spot disease in fluted pumpkin (Telfairia occidentalis Hook F.). J Bio Agric and Healthc. 2017; 7(12):56-62.
Sofowora A. Medicinal plant and traditional medicine in Africa. (2nd Ed.). Ibadan, Nigeria: Nigeria Spectrum Books; 1996. 12 p.
Akalazu JN, Onyike NB, Offong AU, Nwachukwu CU. Investigation of the role of calcium in prolonging the shelf life of fluted pumpkin (Telfairia occidentalis Hook F.). J Biotechnol and Bioinform. 2011; 1(1):1-9.
Inuwa HM, Aimola IA, Muhammad A, Habila N, Okibe P, Latayo M, Ahmed Z. Isolation and determination of omega- 9 fatty acids from Telfairia occidentalis. Inter J Food Nutr Saf. 2012; 1(1):9-14.
Oyewole OA and Abalaka ME. Antimicrobial activities of Telfairia occidentalis (fluted pumpkins) leaf extract against selected intestinal pathogens. J Health Sci. 2012; 2:1-4.
Al-Huqail AA, Behiry SI, Salem MZM, Ali HM, Siddiqui MH, Salem AZM. Antifungal, antibacterial, and antioxidant activities of Acacia saligna (Labill.) H. L. Wendl. Flower Extract: HPLC analysis of phenolic and flavonoid compounds. Mole. 2019; 24 (700):1-14.
Meyer V. A small protein that fights fungi: AFP as a new promising antifungal agent of biotechnological value. Applied Microbiol and Biotechnol. 2008; 78:17–28.
Vinoth B, Manivasagaperumal R, Rajaravindran M. Phytochemical analysis and antibacterial activity of Azadirachta indica Juss. Inter J Res Plant Sci. 2012; 2(3):50-55.
Sofowora A. Medicinal plants and traditional medicinal in Africa. In: Screening plants for bioactive agents. (2nd ed.). Ibadan, Nigeria: Spectrum Books Ltd Sunshine House; 1993. 134-156 p.
Trease GE and Evans WC. Pharmacognosy: a physician guide to herbal medicine. (13th ed.). London: ELBS/Bailliere, Tindall; 1989. 176-180 p.
Cheesbrough M. District laboratory practice in tropical countries. (2nd ed.). Cambridge: Cambridge University Press; 2000; 47-54.
Samie A and Mashau F. Antifungal activities of fifteen Southern African medicinal plants against five Fusarium species. J Med Plants Res. 2013; 7(25):1839-1848.
Sule WF, Okonko IO, Omo-Ogun S, Nwanze JC, Ojezele MO, Ojezele OJ, Alli JA, Soyemi ET, Olaonipekun TO. Phytochemical properties and in-vitro antifungal activity of Senna alata Linn. crude stem bark extract. J Med Plants Res. 2011; 5(2):176-183.
Wu CY, Chen R, Wang XS, Shen B, Yue W, Wu Q. Antioxidant and anti-fatigue activities of phenolic extract from the seed coat of Euryale ferox Salisb. and identification of three phenolic compounds by LC-ESIMS/MS. Mole. 2013; 18:11003-11021.
Dai J and Mumper RJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Mole. 2010; 15:7313-7352.
Salem MZM, El-Hefny M, Ali HM, Elansary HO, Nasser RA, El-Settawy AAA, El Shanhorey N, Ashmawy NA, Salem AZM. Antibacterial activity of extracted bioactive molecules of Schinus terebinthifolius ripened fruits against some pathogenic bacteria. Microbiol Pathol. 2018; 120:119-
Alada ARA. The haematological effects of Telfairia occidentalis diets preparation. Afr J Biomed. 2000; 3:185- 186.
Dina OA, Adedapo AA, Oyinloye OP, Saba AB. Effects of Telfairia occidentalis extract on experimentally induced anaemia in domestic. Afr J Biomed Res. 2006; 3:181-183.
Eseyin O, Ebong P, Eyong E, Awofisayo O, Agboke A. Effect of Telfairia occidentalis on oral glucose tolerance in rats. Afr J Pharm Pharmacol. 2010; 4:368-372.
Oboh G, Nwanna E, Elusiyan C. Antioxidant and antimicrobial Properties of Telfairia occidentalis (Fluted pumpkin) leaf extracts. J Pharmacol Toxicol. 2006; 5(8):539-547.
Eseyin OA, Sattar MA, Rathore HA. A review of the pharmacological and biological activities of the aerial parts of Telfairia occidentalis Hook.f. (Cucurbitaceae). Trop J Pharm Res. 2014; 13(10):1761-1769.
Morcos A and Sarkis T. Histo-pathological alterations of the stomach of guinea pigs following administration of root aqueous extract of Telfairia occidentalis. Eur J Med Sci. 2013; 11(2):1-5.
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