Isolation and Screening of Escherichia coli O157:H7 Bacteriophage
doi.org/10.26538/tjnpr/v4i11.6
Keywords:
Phage therapy, Foodborne disease, Escherichia coli O157:H7, BacteriophageAbstract
The severity of foodborne disease outbreaks caused by Escherichia coli O157:H7 has led to the search for alternative management methods, particularly phage therapy which is currently being revisited. The aim of this study was to isolate and screen lytic E. coli O157:H7 bacteriophages from sewage, for possible use in phage therapy. Sewage sample was collected from the Covenant University sewage treatment plant and enriched with reference E. coli O157:H7, ATCC 10536 for phage isolation. One lytic E. coli O157:H7 bacteriophage was successfully isolated. The isolated bacteriophage was screened using spot assay, to determine its susceptibility to seven previously isolated E. coli isolates. The seven E. coli isolates used in the study were pathotyped using the E. coli O157 latex test kit. The result obtained in this study showed that none of the other E. coli isolates was susceptible to the isolated phage and that none
of the tested isolates belonged to the O157:H7 serogroup. This suggests that the bacteriophage isolated in this study may be specific for E. coli O157:H7. The observed specificity is a useful trait for its use as a potential therapeutic agent for the management of E. coli O157:H7 by phage therapy. Specificity is particularly important given that several harmless E. coli strains that play important roles in maintaining normal intestinal homeostasis, are part of the normal intestinal flora.
References
Mohammed DS, Ahmed EF, Mahmoud AM, El-Baky RMA, John J. Isolation and evaluation of cocktail phages for the control of multidrug-resistant Escherichia coli serotype O104: H4 and E. coli O157: H7 isolates causing diarrhea. FEMS Microbiol Lett. 2018; 365(2):1-7.
Clements A, Young JC, Constantinou N, Frankel G. Infection strategies of enteric pathogenic Escherichia coli. Gut Microb. 2012; 3(2):71-87.
Gyles CL. Shiga toxin-producing Escherichia coli: an overview. J Anim Sci. 2007; 85(13):45-62.
Lupindu AM. Epidemiology of Shiga toxin-producing Escherichia coli O157:H7 in Africa in review. S Afr J Infect Dis. 2018; 33:24-30.
Hoffmann S, Maculloch B, Batz M. Economic burden of major foodborne illnesses acquired in the United States Economic Research Services, US Department of Agriculture, Washington, DC, EIB. 2015. 140 p.
Litt PK and Jaroni D. Isolation and physiomorphological characterization of Escherichia coli O157:H7-infecting bacteriophages recovered from beef cattle operations. Int J Microbiol. 2017; 2017:1-12.
Bergh O, Børsheim KY, Bratbak G, Heldal M. High abundance of viruses found in aquatic environments. Nature 1989; 340(6233):467-468.
Barrera-Rivas CI, Valle-Hurtado NA, Gonzalez-Lugo GM, Baizabal-Aguirre VM, Bravo-Patino A, Cajero-Juarez M, Valdez-Alarcon JJ. Bacteriophage therapy: an alternative for the treatment of Staphylococcus aureus infections in animals and animal models. Front Staphylococcus aureus. 2017; 19:170-201.
Lin DM, Koskella B, Lin HC. Phage therapy: an alternative to antibiotics in the age of multi-drug resistance. World J Gastrointest Pharmacol Ther. 2017; 8(3):162-173.
Elbreki M, Ross RP, Hill C, O’Mahony J, Mcauliffe O, Coffey A. Bacteriophages and their derivatives as biotherapeutic agents in disease prevention and treatment. J Viruses. 2014; 2014:1-20.
Raya RR, Varey P, Oot RA, Dyen MR, Callaway TR, Edrington TS, Kutter EM, Brabban AD. Isolation and characterization of a new t-even bacteriophage, CEV1, and determination of its potential to reduce Escherichia coli O157:H7 levels in sheep. Appl Environ Microbiol. 2006;
(9):6405-6410.
Viazis S, Akhtar M, Feirtag J, Diez-Gonzalez F. Reduction of Escherichia coli O157:H7 viability on leafy green vegetables by treatment with a bacteriophage mixture and trans-cinnamaldehyde. Food Microbiol. 2011; 28(1):149- 157.
Petty NK, Evans TJ, Fineran PC, Salmond GP. Biotechnological exploitation of bacteriophage research. Trends Biotechnol. 2007; 25(1):7-15.
Duckworth DH. Who discovered bacteriophage? Bacteriol. Rev. 1976; 40(4):793-802. 15. Manohar P, Tamhankar AJ, Lundborg CS, Ramesh .
Isolation, characterization and in vivo efficacy of Escherichia phage myPSH1131. PLoS ONE 2018; 13(10):1-17.
Ganeshan SD and Hosseinidoust Z. Phage therapy with focus on the human microbiota. Antibiotics 2019; 8(131):1- 19.
Fernandez L, Gutierrez D, Rodriguez A, Garcia P. pplication of bacteriophages in the agro-food sector: a long way toward approval. Front Cell Infect Microbiol. 2018; 8(296):1-5.
Lewis R and Hill C. Overcoming barriers to phage application in food and feed. Curr. Opin Biotechnol. 2020; 61:38-44.
Lukman C, Yonathan C, Magdalena S, Waturangi DE. solation and characterization of pathogenic Escherichia coli bacteriophages from chicken and beef offal. BMC Res. Notes. 2020; 13(8):1-7.
Zbikowska K, Michalczuk M, Dolka B. The use of bacteriophages in the poultry industry. Anim. 2020; 10(872):1-18.
Kazi M and Annapure US. Bacteriophage biocontrol of foodborne pathogens. J Food Sci Technol. 2016; 53(3):1355-1362.
Wang L, Qu K, Li X, Cao Z, Wang X, Li Z, Song Y, Xu Y. Use of bacteriophages to control Escherichia coli O157:H7 in domestic ruminants, meat products, fruits and vegetables. Foodborne Pathog Dis. 2017; 14(9):483-493.
Cerveny K, Depaola A, Duckworth D, Gulig P. Phage therapy of local and systemic disease caused by Vibrio vulnificus in iron-dextran-reated mice. Infect Immun. 2002; 70(11):6251-6262.
Santos S, Carvalho CM, Sillankorva S, Nicolau A, Ferreira EC, Azeredo J. The use of antibiotics to improve phage detection and enumeration by the double-layer agarechnique. BMC Microbiol. 2009; 9(148):1-10.
Yildirim Z, Sakin T, Coban F. Isolation of anti-Escherichia coli O157:H7 bacteriophages and determination of their host ranges. Turk J Agric Food Sci Technol. 2018; 6(9):1200-1208.
Synnott AJ, Kuang Y, Kurimoto M, Yamamichi K, Iwano H, Tanji Y. Isolation from sewage influent and characterization of novel Staphylococcus aureus bacteriophages with wide host ranges and potent lytic capabilities. Appl Environ Microbiol. 2009; 75(13):4483-4490.
Viazis S, Akhtar M, Feirtag J, Brabban AD, Diez-Gonzalez F. Isolation and characterization of lytic bacteriophages against nterohaemorrhagic Escherichia coli. J Appl Microbiol. 2011; 110(5):1323-1331.
Naghavi NS, Golgoljam M, Akbari M. Effect of three sewage isolated bacteriophages on the multidrug resistant pathogenic bacteria. J Biol Sci. 2013; 13(5):422-426.
Bikram G, Lomas A, Sachana A, Manoj R, Anjita R, Sunita G, Rameshwar A. Isolation of bacteriophage from Guheswori sewage treatment plant capable of infecting pathogens. Res Pharm Health Sci. 2018; 4(2):465-470.
Manohar P, Tamhankar AJ, Lundborg CS, Nachimuthu R. Therapeutic characterization and efficacy of bacteriophage cocktails infecting Escherichia coli, Klebsiella pneumoniae, and Enterobacter Species. Front Microbiol. 2019; 10(574):1-12.
Oot RA, Raya RR, Callaway TR, Edrington TS, Kutter EM, Brabban AD. Prevalence of Escherichia coli O157 and O157: H7-infecting bacteriophages in feedlot cattle faeces. Lett Appl Microbiol. 2007; 45(4):445-453.
Raya RR, Oot RA, Moore-Maley B, Wieland S, Callaway TR, Kutter EM, Brabban AD. Naturally resident and exogenously applied T4like and T5-like bacteriophages can reduce Escherichia coli O157:H7 levels in sheep guts. Bacteriophage. 2011; 1(1):15-24.
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
