Green Synthesis of Silver Nanoparticles with Silkworm Excretions and their Potential Antibiofilm Activity on Oral Bacteria

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Published: 2025-07-31
DOI: https://doi.org/10.26538/tjnpr/v9i7.39
Keywords:
Silkworm excreta, Reverse transcription polymerase Chain reaction, Oral bacteria, Extracellular polysaccharide, Antibacterial activity

Main Article Content

Qutaiba A Ismael
Department of Pharmaceutics, College of Pharmacy, Al-Farahidi University, Baghdad, Iraq
Mohammed S Ali
Department of Pharmaceutics, College of Pharmacy, Al-Farahidi University, Baghdad, Iraq
Raghda H Aljorani
Department of Clinical Chemistry, Faculty of Pharmacy, Al-Rafidain University College, Baghdad, Iraq.
Ali Al-Shammari
Department of Pharmaceutics, Faculty of Pharmacy, Al-Nahrain University College, Baghdad, Iraq
Mohammed H Al-Bayati
Department of Pharmaceutics, College of Pharmacy, Al-Farahidi University, Baghdad, Iraq

Abstract

ABSTRACT
Dental caries is a chronic infectious disease that causes tooth loss and root breakdown in children and young adults, with a prevalence ranging from 60-80% in children and almost 100% in adults. Green synthesis of metallic nanoparticles is a highly economical and practical way to reduce antimicrobial resistance. Green synthesis of silver nanoparticles (AgNPs) was performed using silkworm pellets and validated by characterization studies (UV-visible spectroscopy, XRD, FTIR, and SEM). Antibacterial activity was evaluated against three oral bacterial strains (S. mutans, S. oralis, and S. gingivalis) by MIC, time-kill, and antibiofilm assays. qRT-PCR was performed to evaluate the expression of biofilm-formation genes (gftB, gftC, gftD, srtA, and comD). Scanning electron microscopy (SEM) showed that the synthesized AgNPs were between 40 and 55 nm (in). The synthesized AgNPs were found to reduce biofilms and exopolysaccharides in accordance with the MIC and agar well diffusion. Antibacterial activity was found to be highly significant with NPs, as evidenced by the downregulation of the qRT-PCR genes. The green-synthesized AgNPs from silkworm excreta exhibited antibacterial activity against oral bacteria, with a high percentage of biofilm inhibition and downregulation of biofilm-forming genes.

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Vol. 9 No. 7 (2025): Tropical Journal of Natural Product Research (TJNPR)

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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Green Synthesis of Silver Nanoparticles with Silkworm Excretions and their Potential Antibiofilm Activity on Oral Bacteria. (2025). Tropical Journal of Natural Product Research , 9(7), 3150 – 3159. https://doi.org/10.26538/tjnpr/v9i7.39

References

1. Selwitz RH, Ismail AI, Pitts NB. Dental caries. The Lancet. 2007;369(9555):51-59. DOI: https://doi.org/10.1016/S0140-6736(07)60031-2

2. Yu OY, Lam WY-H, Wong AW-Y, Duangthip D, Chu C-H. Nonrestorative management of dental caries. Dent. J. 2021;9(10):121. DOI: https://doi.org/10.3390/dj9100121

3. Featherstone J. The continuum of dental caries—evidence for a dynamic disease process. J. Dent. Res. 2004;83(1_suppl):39-42. DOI: https://doi.org/10.1177/154405910408301s08

4. Thomas B, Ramesh A. Nanotechnol. Dent. Implantol. Nanomaterials in Dental Medicine: Springer; 2023. p. 159-175. DOI: https://doi.org/10.1007/978-981-19-8718-2_9

5. Hannig M, Hannig C. Nanotechnology and its role in caries therapy. Adv. Dent. Res. 2012;24(2):53-7. DOI: https://doi.org/10.1177/0022034512450446

6. Hoque J, Konai MM, Sequeira SS, Samaddar S, Haldar J. Antibacterial and antibiofilm activity of cationic small molecules with spatial positioning of hydrophobicity: an in vitro and in vivo evaluation. J. Med. Chem. 2016;59(23):10750-62. DOI: https://doi.org/10.1021/acs.jmedchem.6b01435

7. Zhang B, Zhao M, Tian J, Lei L, Huang R. Novel antimicrobial agents targeting the Streptococcus mutans biofilms discovery through computer technology. Front. Cell. Infect. Microbiol. 2022;12:1808. DOI: https://doi.org/10.3389/fcimb.2022.1065235

8. Kouidhi B, Al Qurashi YMA, Chaieb K. Drug resistance of bacterial dental biofilm and the potential use of natural compounds as alternative for prevention and treatment. Microb. Pathog. 2015;80:39-49. DOI: https://doi.org/10.1016/j.micpath.2015.02.007

9. Karibasappa G, Sujatha A. Antibiotic resistance-a concern for dentists. J. Dent. Med. Sci. 2014;13(2):112-118. DOI: https://doi.org/10.9790/0853-1324112118

10. Kaur N, Sahni P, Singhvi A, Hans MK, Ahluwalia AS. Screening the drug resistance property among aerobic pathogenic microorganisms of dental caries in north-western Indian population: a preliminary study. J. Clin. Diagn. Res. 2015;9(7):ZC05. DOI: https://doi.org/10.7860/JCDR/2015/11989.6143

11. Solomon SL, Oliver KB. Antibiotic resistance threats in the United States: stepping back from the brink. Am. Fam. Physician. 2014;89(12):938-41.

12. Chater AM, Family H, Abraao LM, Burnett E, Castro-Sanchez E, Du Toit B, et al. Influences on nurses' engagement in antimicrobial stewardship behaviours: a multi-country survey using the Theoretical Domains Framework. J. Hosp. Infect. 2022;129:171-80. DOI: https://doi.org/10.1016/j.jhin.2022.07.010

13. Piddock LJ. The crisis of no new antibiotics—what is the way forward? Lancet Infect. Dis. 2012;12(3):249-253. DOI: https://doi.org/10.1016/S1473-3099(11)70316-4

14. Bakhshi B, Malla S, Gowda LS. Garlic Mediated Green Synthesis of Silver Nanoparticles as Antifungal Agents against Magnaporthe oryzae. Indian J. Pharm. Educ. Res. 2022;56(4). DOI: https://doi.org/10.5530/ijper.56.4.207

15. Sudhakar M, Raman BV. Bactericidal and Antibiofilm Activity of Tannin Fractions Derived from Azadirachta against Streptococcus mutans. Asian J. Appl. Sci. 13: 132-143 DOI: 103923/ajaps. 2020;143. DOI: https://doi.org/10.3923/ajaps.2020.132.143

16. Sutthisa W, Sophachan H, Pimvichai P, Srisawad N. Antimicrobial Efficiency and Chemical Composition of Jackfruit (Artocarpus heterophyllus Lam.) Extracts from Different Plant Parts. Trop. J. Nat. Prod. Res. 2025;9(3):925-32. DOI: https://doi.org/10.26538/tjnpr/v9i3.6

17. Thammawithan S, Siritongsuk P, Nasompag S, Daduang S, Klaynongsruang S, Prapasarakul N, Patramanon R. A biological study of anisotropic silver nanoparticles and their antimicrobial application for topical use. Vet. Sci. 2021;8(9):177. DOI: https://doi.org/10.3390/vetsci8090177

18. Mishra B, Wang X, Lushnikova T, Zhang Y, Golla RM, Narayana JL, et al. Antibacterial, antifungal, anticancer activities and structural bioinformatics analysis of six naturally occurring temporins. Peptides. 2018;106:9-20. DOI: https://doi.org/10.1016/j.peptides.2018.05.011

19. Packiavathy IASV, Priya S, Pandian SK, Ravi AV. Inhibition of biofilm development of uropathogens by curcumin–an anti-quorum sensing agent from Curcuma longa. Food Chem. 2014;148:453-460. DOI: https://doi.org/10.1016/j.foodchem.2012.08.002

20. Gulube Z, Patel M. Effect of Punica granatum on the virulence factors of cariogenic bacteria Streptococcus mutans. Microb. Pathog. 2016;98:45-9. DOI: https://doi.org/10.1016/j.micpath.2016.06.027

21. Livak KJ, Schmittgen TD. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods. 2001;25(4):402-408. DOI: https://doi.org/10.1006/meth.2001.1262

22. Augustine R, Rajarathinam K. Synthesis and characterization of silver nanoparticles and its immobilization on alginate coated sutures for the prevention of surgical wound infections and the in vitro release studies. 2012.

23. Thamilselvi V, Radha K. Synthesis of silver nanoparticles from Pseudomonas putida NCIM 2650 in silver nitrate supplemented growth medium and optimization using response surface methodology. Dig. J. Nanomater. Biostruct. 2013;8(3).

24. Bhanumathi R, Vimala K, Shanthi K, Thangaraj R, Kannan S. Bioformulation of silver nanoparticles as berberine carrier cum anticancer agent against breast cancer. New J Chem. 2017;41(23):14466-77. DOI: https://doi.org/10.1039/C7NJ02531A

25. Jeevanandam J, Barhoum A, Chan YS, Dufresne A, Danquah MK. Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein J. Nanotechnol. 2018;9(1):1050-1074. DOI: https://doi.org/10.3762/bjnano.9.98

26. Wang Y, Zeng Y, Wang Y, Li H, Yu S, Jiang W, et al. Antimicrobial peptide GH12 targets Streptococcus mutans to arrest caries development in rats. J. Oral Microbiol. 2019;11(1):1549921. DOI: https://doi.org/10.1080/20002297.2018.1549921

27. Gazoni VF, Balogun SO, Arunachalam K, Oliveira DM, Cechinel Filho V, Lima SR, et al. Assessment of toxicity and differential antimicrobial activity of methanol extract of rhizome of Simaba ferruginea A. St.-Hil. and its isolate canthin-6-one. J. Ethnopharmacol. 2018;223:122-134. DOI: https://doi.org/10.1016/j.jep.2018.05.014

28. Li L, Sun J, Xia S, Tian X, Cheserek MJ, Le G. Mechanism of antifungal activity of antimicrobial peptide APP, a cell-penetrating peptide derivative, against Candida albicans: intracellular DNA binding and cell cycle arrest. Appl. Microbiol. Biotechnol. 2016;100:3245-53. DOI: https://doi.org/10.1007/s00253-015-7265-y

29. Koo H, Falsetta M, Klein M. The exopolysaccharide matrix: a virulence determinant of cariogenic biofilm. J. Dent. Res. 2013;92(12):1065-73. DOI: https://doi.org/10.1177/0022034513504218

30. Hanada N, Kuramitsu HK. Isolation and characterization of the Streptococcus mutans gtfC gene, coding for synthesis of both soluble and insoluble glucans. Infect. Immun. 1988;56(8):1999-2005. DOI: https://doi.org/10.1128/iai.56.8.1999-2005.1988

31. Zhong H, Xie Z, Wei H, Zhang S, Song Y, Wang M, Zhang Y. Antibacterial and antibiofilm activity of Temporin-GHc and Temporin-GHd against cariogenic bacteria, Streptococcus mutans. Front. Microbiol. 2019;10:2854. DOI: https://doi.org/10.3389/fmicb.2019.02854