Antihypertensive and Vasorelaxant Effects of Cistanche phelypaea Leaves through Inhibition of Receptor-Operated Calcium Channels in Rats
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Abstract
Cistanche phelypaea (family: Orobanchaceae) is a medicinal plant traditionally used for its vasorelaxant, diuretic, and sedative properties, highlighting its potential relevance in the management of hypertension. The present study aimed to evaluate the antihypertensive and vasorelaxant effects of Cistanche phelypaea leaves and its impact on angiotensin-converting enzyme 2 (ACE-2). The aqueous leaf extract of Cistanche phelypaea (CPAE) was prepared and its antihypertensive effect (200 mg/kg) was examined using a hypertensive rat model induced by oral administration of L-NAME (60 mg/kg bw), additionally, we assessed its vasorelaxant potential and its impact on the stimulation or inhibition of ACE-2 in isolated rat thoracic aorta. The results indicate that CPAE significantly lowered systolic (p<0.0001), diastolic (p<0.01), and mean arterial pressure (p<0.01) without affecting normotensive rats. The data showed that CPAE mediates its antihypertensive effect via vasodilatory activity. Furthermore, the vasorelaxant capacity of CPAE seems to be mediated through receptor-operated calcium channels (ROCCs). However, CPAE had no effect on ACE-2. In conclusion, the study demonstrates that the aqueous leaf extract of Cistanche phelypaea possesses a significant antihypertensive and vasorelaxant effects, primarily through the inhibition of Ca2+ entry.
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