Micromorphological Characters in Wild Medicinal Species of Dioscorea (Dioscoreaceae) http://www.doi.org/10.26538/tjnpr/v7i3.24

Main Article Content

Emmanuel Odoh
Adedapo A. Adeniran
Mubo A. Sonibare

Abstract

Adulteration, misidentification, and substitution of wild Dioscorea species are common in Nigeria's herbal market and among traditional herbal practitioners. The present investigation reports a comparative micromorphological study of three wild species of Dioscorea L. used in ethnomedicine in South-western Nigeria to elucidate taxonomically significant characters, which would aid species identification. Physical and chemical methods were used in obtaining the epidermal leaf surfaces of the Dioscorea species. Leaves of D. hirtiflora Benth. and D. bulbifera L. were amphistomatic, whereas D. dumetorum (Kunth) Pax were hypostomatic. Diagnostic foliar epidermal characters include striated cell wall; presence of raphides in D. hirtiflora and D. dumetorum and secretory glands in D. dumetorum (wild) and D. hirtiflora. Glandular trichomes were observed in all species in addition to simple, elongated, unicellular trichomes in D. dumetorum and stellate trichomes in D. hirtiflora. Epidermal cells mainly were
polygonal, straight to slightly wavy but deeply undulating in D. dumetorum. The largest and smallest mean epidermal cell sizes were obtained on the adaxial surfaces of D. bulbifera (mauve) and D. dumetorum (wild), respectively. Micromorphological characters in the Dioscorea species studied are taxonomically significant for species identification and could serve as diagnostic taxonomic tools for their standardization. A key for the identification of species is provided.

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How to Cite
Odoh, E., Adeniran, A. A., & Sonibare, M. A. (2023). Micromorphological Characters in Wild Medicinal Species of Dioscorea (Dioscoreaceae): http://www.doi.org/10.26538/tjnpr/v7i3.24. Tropical Journal of Natural Product Research (TJNPR), 7(3), 2624-2630. https://www.tjnpr.org/index.php/home/article/view/1786
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Author Biography

Adedapo A. Adeniran, Department of Pharmacognosy, University of Ibadan, Ibadan, Nigeria

Department of Pharmacognosy and Natural Medicine, University of Calabar, Calabar, Nigeria

 

How to Cite

Odoh, E., Adeniran, A. A., & Sonibare, M. A. (2023). Micromorphological Characters in Wild Medicinal Species of Dioscorea (Dioscoreaceae): http://www.doi.org/10.26538/tjnpr/v7i3.24. Tropical Journal of Natural Product Research (TJNPR), 7(3), 2624-2630. https://www.tjnpr.org/index.php/home/article/view/1786

References

Sasikumar B, Swetha VP, Parvathy VA, and Sheeja TE. Advances in Adulteration and Authenticity Testing of Herbs

and Spices in Advances in Food Authenticity Testing. Woodhead Publishing Series in Food Science, Technology

and Nutrition. 2016; Chapter 22: 585-624.

Dastagir G, Ahmad I, and Uza NU. Micromorphological evaluation of Daphne mucronata Royle and Myrtus communis L. using scanning electron microscopic techniques. Micros. Res. Tec. 2022; 85(3):1120-1134.

Olaniyi MB, Lawal IO, and Adeniyi M. Chemoscopic, Macromorphological and Micromorphological Evaluation of the leaves of Crescentia cujete linn Nig. J. Nat. Prod. and Med. 2019; 23:63-68.

Singh S. and Verma, D. Foliar Epidermal Study on Selected Medicinal Plants Used in Homeopathy. Pharmacog. Res. 2021; 13:75-81.

Sonibare MA, Jayeola AA, and Egunyomi A. Comparative leaf anatomy of Ficus Linn. Species (Moraceae) from Nigeria. J. Appl. Sci. 2006; 6(15):3016-3025.

Ardhamy SD, Fangirl MA, and Novaryatin S. Pharmacognostic study of Bawang Dayak (Eleutherine bulbous (Mill.) Urb.) and its clay mask against Acnecausing Bacteria. Trop. J. Nat. Prod.Res. 2022; 6(10): 1614- 1621.

Odimegwu JI, Yadav RK, Ogbonnia SO, Odukoya OA and Sangwan NS. In vitro Diosgenin Augementation in

Microtubers of Dioscorea floribunda (M. Martens & Galeotti). Trop. J. Nat. Prod. Res. 2017; 1(2):69-75.

Tamiru M, Becker HC, and Mass BL. Diversity, distribution and management of yamland races (Dioscorea spp.) in southern Ethiopia. Genet. Resour. Crop Evol. 2008; 55:115-131.

Stephen JM. Yams-Dioscorea spp. Horticultural Sciences Department, Cooperative Extension Service, Institute of Food and Agricultural Sciences (IFAS), University of Florida, Gainesville, 2009; FL, 32611.

Adeniran AA. and Sonibare, MA. In vitro antioxidant activity, brine shrimp lethality and assessment of bioactive constituents of three wild Dioscorea species. Food Measure. 2017; 11:685–695.

Ezeabara CA, and Anona RO. Comparative Analyses of Phytochemical and Nutritional Compositions of Four Species of Dioscorea. Acta Scientific Nutritional Health. 2018; 2(7): 90-94

Fasaanu OP, Oziegbe M, and Oyedapo OO. Investigations of activities of alkaloid of trifoliate yam (Dioscorea

dumetorum) (Kunth) Pax. Ife J. Sci. 2013; 15(2):251-262.

Hamon P, Dumont R, Zoudjihekpon J, Tio-Toure B, and Hamon S. Wild yams of West Africa Morphological Characteristics. Ed. De l’orstom, Paris. 1995; Chapter 12:385-400.

Adeleye A, and Ikotun T. Antifugal activity of dihydrodioscorine extracted from a wild variety of Dioscorea bulbifera L. J. Basic Microbiol. 1989; 29(5):265- 267.

Adeniran AA, Sonibare MA, and Shashi K. Comparative analysis of the constituents of two cultivars of Dioscorea

dumetorum (kunth) Pax. and their molecular barcoding. Biochemical Systematics and Ecology. 2020; 93:104140.

Iwu MM, Okunji CO, Ohiaeri GO, Akah P, and Corley D. Hypoglycaemic activity of dioscoretine from tubers of Dioscorea dumetorum. Planta Med. 1990; 56:264-267.

Gao HY, Kuroyanagi M, Wu L, Kawahara N, Yasuno T, and Akamura Y. Antitumour Promoting constituents from Dioscorea bulbifera in JB6 mouse epidermal cells. Biol. Pharm. Bull. 2002; 25(12): 1241-1243.

Ashidi JS, Houghton PJ, Hylands PJ, and Efferth T. Ethnobotanical survey and cytotoxicity testing of plants of south-western Nigeria used to treat cancer, with isolation of cytotoxic constituents from Cajanus cajan Millsp. leaves. J.

Ethnopharmacol. 2010; 128:501-512.

Sonibare MA, and Abegunde RB. In vitro antimicrobial and antioxidant analysis of Dioscorea dumetorum (Kunth) Pax

and Dioscorea hirtiflora (Linn.) and their bioactive metabolites from Nigeria. J. Appl. Biosci. 2012.; 51:3583- 3590.

Parveen I, Gafner S, Techen N, Susan J. Murch SJ, and Khan IA. DNA Barcoding for the Identification of Botanicals in Herbal Medicine and Dietary Supplements: Strengths and Limitations. Planta Med. 2016; 82:1225– 1235.

Adeniran AA, and Sonibare MA. Exploitation of Petiole, Nodal Segment, bulbil and tuber Anatomy for species identification in Dioscorea Linn. Species from Oyo and Ekiti States-Southwestern Nigeria. Nigerian Journal of Natural Products and Medicine. 2016; 20:43-51.

Metcalfe CR, and Chalk RL. Anatomy of the Dicotyledons: Leaves, Stem and wood in Relation to Taxonomy with Notes on Economic Uses. 1979; 2nd ed. Oxford, UK: Clarendon Press.

Mohajer S, Taha RM, Mohamed N and Razak UNA. Baybean (Canavalia rosea (Sw.) DC.); organogenesis, morphological and anatomical studies. 2017; Gayana Bot. 74(1): 1-11

Chaudhary N, and Imran M. Comparative study of stomata in some members of Malvaceae and Euphorbiaceae, Pak. .Pl. Sci. 1997; 3(1): 33-45.

Sonibare MA, and Adeniran AA. Comparative micromorphological study of wild and micropropagated Dioscorea bulbifera Linn. Asian Pac. J. Trop. Biomed. 2014; 4(3):176-183.

Adeniran AA, Ntamanwuna EC, and Bassey VO. Microscopical Characterization and Physicochemical Standardization of leaves, stems and roots of Spondias mombin L. (Anacardiaceae). Nigerian Journal of Pharmaceutical Research. 2021; 17(1);15-25.

Shah GL, and Gopal BV. Some observations on the diversity of stomata and trichomes in six species of Dioscorea. Ann. Bot. 1972; 36:997-1004.

Abdulrahaman AA, Egbedo FO, and Oladele FA. Stomatal complex types, stomatal density, and the stomatal index in some species of Dioscorea. Arch. Biol. Sci. Belgrade. 2009; 61(4): 847-851.

Hameed TG, Dastagir F, and Hussain F. Nutritional and elemental analyses of some selected medicinal plants of the family Polygonaceae. Pak. J. Bot. 2008; 40(6):2493-2502.

Tang M, Hu YX, Lin JX, and Jin XB. Developmental mechanism and distribution pattern of stomata clusters in Begonia peltatifolia. Acta Bot. Sinica. 2002; 44:384-390.

Yadav RP. Stomatal abnormalities in Pennisetum. Curr. Sci. 1969; 38:441-442.

Shah GL, and Gopal BV. Structure and development of stomata on the vegetative and floral organs of some Amarylliaceae. Ann. Bot. 1970; 34:737-749.

Chengqi A, Chuangxing Y, and Zhang H. A systematic investigation of leaf epidermis in Camellia using light microscopy. Biologia Bratislava. 2007; 62(2):157-162.

Bobek VB, Heiden G, Oliveira CF, Almeida VP, Paula JP, Farago PV, and Budel JM. Comparative analytical micrographs of vassouras (Baccharis, Asteraceae). Revista Brasileira de Farmacognosia. 2016; 26(6): 665-672.

Budel JM, Raman V, Monteiro LM, Almeida VP, Bobek VB, Heiden G, Takeda IJM, and Khan IA. Foliar Anatomy and Microscopy of six Brazilian species of Baccharis (Asteraceae). Microsc. Res. Tech. 2018; 1-11.

Prychid CJ, and Rudall PJ. Calcium oxalate crystals in Monocotyledons: a review of their structure and systematic. Ann. Bot. 1999; 84: 725-739.

Ayensu ES. Anatomy of Monocotyledons. Vol. 6 In: Metcalfe CR, editor. Dioscorales. Oxford UK: 1972; Clarendon Press.

Glas JJ. Schimmel BC. Alba JM. Escobar-bravo R. Schuurink RC. and Kant MR. Plant glandular trichomes as targets for breeding or engineering of resistance to herbivores. Int. J. Mol. Sci. 2012; 13: 17077–17103.

Dhankhar R, Regmi K, Kawatra A. and Gulati P. Trichomics: Trichomes as Natural Chemical Factories. Phytochemical Genomics. 2023; 379–402.

Adedeji O, Ajuwon OY, and Babawale OO. Foliar epidermal studies, organographic distribution and taxonomic importance of trichomes in the family Solanaceae. Int. J. Bot. 2007; 3(3): 276-282.

Adeniran AA, Sonibare MA, Rajacharya GH, and Kumar S. Assessment of genetic fidelity of Dioscorea bulbifera L.

and Dioscorea hirtiflora Benth. and medicinal bioactivity produced from the induced tuberous roots. Plant Cell Tissue

Organ Cult. 2018; 132:343-357.

Zongram O, Ruangrungsi N, Palannuvj C. and Rungsihirunrat K. Leaf constant numbers of selected Gardenia species in Thailand. J. Health Res. 2017; 31(1):69-75.

Gul S, Ahmad, M, Zafar M, Bahadur S, Celep F, Sultana S, Begum N, Hanif U, Zamanm W, Shuaib M, Ayaz A. Taxonomic significance of foliar epidermal morphology in Lamiaceae from Pakistan. Microsc. Res.Tech. 2019:1–22.