Antioxidant, Anti-Collagenase, Anti-Elastase and Anti-Tyrosinase Activities of an Aqueous Cosmos caudatus Kunth (Asteraceae) Leaf Extract


  • Zakaria NN. Azwanida Faculty of Agro‐based Industry, Universiti Malaysia Kelantan, Jeli 17600, Kelantan, Malaysia
  • Okello E. Jonathan Human Nutrition Research Centre, Institute of Cellular Medicine, The Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, UK
  • Howes Melanie-Jaynes Natural Capital and Plant Health, Royal Botanic Gardens, Kew, Richmond TW9 3AB, UK


Ionizing radiation, Oxidative stress, DNA Fragmentation, Comet assay


Ionizing radiation (IR) induces many cellular changes by direct and indirect interactions, which alters multiple cellular processes, causing DNA damage and reactive oxygen species-induced biochemical disturbances. This study aimed at investigating the effect of various doses of computed Tomography (CT) scanning on the fragmentation of DNA and oxidative stress in healthy young adults. The DNA damages associated with different doses of CT scan was explored using alkaline comet assay. Serum concentration of malondialdehyde (MDA) in each sample was measured using enzyme-linked immunosorbent assay (ELISA). There was a significantly higher percent of DNA fragmentation in the blood samples subjected to high dose of radiation (120 kV-210 mAs- 21sec) than those exposed to lower doses. Furthermore, the serum concentration of MDA was proportional to the intensity of radiation. Therefore, DNA fragmentation can be associated with high doses of CT radiation, maybe due to radiation-induced oxidative stress. 


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How to Cite

Azwanida, Z. N., Jonathan, O. E., & Melanie-Jaynes, H. (2020). Antioxidant, Anti-Collagenase, Anti-Elastase and Anti-Tyrosinase Activities of an Aqueous Cosmos caudatus Kunth (Asteraceae) Leaf Extract: Tropical Journal of Natural Product Research (TJNPR), 4(12), 1045–1049. Retrieved from