Comparative Phytochemical and FTIR Evaluation of Morinda lucida Leaves from Two Locations within the University of Ibadan
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Abstract
Morinda lucida is widely used in African traditional medicine; however, variability in its phytochemical composition may affect its medicinal quality. This study aimed to comparatively evaluate the phytochemical profile and Fourier-transform infrared (FTIR) characteristics of M. lucida leaves obtained from two locations within the University of Ibadan: Animal House, near the Faculty of Basic Medical Sciences, and Teaching and Research Farm. The solvent extracts, obtained by macerating pulverized dried leaves with chloroform and acetone, were evaluated using phytochemical screening, thin layer chromatography, and infrared spectroscopy. Comparisons were made between the datasets. Phytochemical screening revealed more variations in the phytochemical composition of the acetone extracts of both samples compared with the chloroform extracts. The infrared spectra of chloroform extracts appeared to match closely; however, noticeable variations in the spectra of acetone extracts. The results indicated possible variations in the phytochemical composition of M. lucida (leaves) collected from the same geographical locations. This emphasizes the need for enhanced standardization protocols and quality assurance of M. lucida morphological parts used for herbal formulations, even within the same geographic area.
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1.Hostettmann K, Marston A. Twenty Years of Research into Medicinal Plants: Results and Perspectives. Phytochem Rev. 2002; 1(3):275–285. https://doi.org/10.1023/A:1026046026057
2.Chaachouay N, Zidane L. Plant-derived natural products: A source for drug discovery and development. Drugs Drug Cand. 2024; 3(1):184–207. https://doi.org/10.3390/ddc3010011
3.Cushnie TPT, Lamb AJ. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents. 2005; 26(5):343–356. https://doi.org/10.1016/j.ijantimicag.2005.09.002
4.Sofowora A, Ogunbodede E, Onayade A. The role and place of medicinal plants in the strategies for disease prevention. Afr J Tradit Complement Altern Med. 2013; 10(5):210–229. https://doi.org/10.4314/ajtcam.v10i5.2
5.Chauke PK, Ndhlovu PT, Semenya SS, Maroyi A. Ethnobotanical appraisal of wild fruit species used in Mpumalanga Province, South Africa: a systematic review. South Afr J Bot. 2024; 168:114–130. https://doi.org/10.1016/j.sajb.2024.06.047
6.Adeleye OO, Ajamu MA. Traditional and medicinal uses of Morinda lucida. J Med Plant Stud. 2018; 6(2):249–254.
7.Adewole KE, Attah AF, Adebayo JO. Morinda lucida Benth (Rubiaceae): A review of its ethnomedicine, phytochemistry and pharmacology. J Ethnopharmacol. 2021; 276:114055. https://doi.org/10.1016/j.jep.2021.114055
8.Sabindo NH, Yatim RM, Thirumulu KP. Phytochemical composition of Clinacanthus nutans based on factors of environment, genetics and postharvest processes: A review. Biomedicine (Taipei). 2024; 14(2):1–11. https://doi.org/10.37796/2211-8039.1451
9.Martins-Noguerol R, Matías L, Pérez-Ramos IM, Moreira X, Francisco M, Pedroche J, DeAndrés-Gil C, Gutiérrez E, Salas JJ, Moreno-Pérez AJ, Davy AJ, Muñoz-Vallés S, Figueroa ME, Cambrollé J. Soil physicochemical properties associated with the yield and phytochemical composition of the edible halophyte Crithmum maritimum. Sci Total Environ. 2023; 869:161806. https://doi.org/10.1016/j.scitotenv.2023.161806
10.Biondi F, Balducci F, Capocasa F, Visciglio M, Mei E, Vagnoni M, Mezzetti B, Mazzoni L. Environmental conditions and agronomical factors influencing the levels of phytochemicals in Brassica vegetables responsible for nutritional and sensorial properties. Appl Sci. 2021; 11(4):1927. https://doi.org/10.3390/app11041927
11.Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman and Hall; 1998. p. 1–3, 60-90.
12.Nwafor I, Nwafor C, Manduna I. Constraints to cultivation of medicinal plants by smallholder farmers in South Africa. Horticulturae. 2021; 7(12):531. https://doi.org/10.3390/horticulturae7120531
13.Aulton ME. Pharmaceutics: The Science of Dosage Form Design. 2nd ed. London: Churchill Livingstone; 2000. p. 353.
14.Muyumba NW, Mutombo SC, Sheridan H, Nachtergael A, Duez P. Quality control of herbal drugs and preparations: The methods of analysis, their relevance and applications. Talanta Open. 2021; 4:100070. https://doi.org/10.1016/j.talo.2021.100070
15.Wagner H, Bladt S. Plant Drug Analysis: A Thin Layer Chromatography Atlas. 2nd ed. Berlin: Springer-Verlag; 1996. p. 1–8, 195-200.
16.Evans WC. Trease and Evans’ Pharmacognosy. 13th ed. London: Bailliere Tindall; 1989. p. 378–546.
17.Ayoola GA, Coker HA, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC, Atangbayila TO. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Trop J Pharm Res. 2008; 7(3):1019–1024. https://doi.org/10.4314/tjpr.v7i3.14686
18.Kowalska T, Sajewicz M. Thin-layer chromatography (TLC) in the screening of botanicals—its versatile potential and selected applications. Molecules. 2022; 27(19):6607. https://doi.org/10.3390/molecules27196607
19.Mayasari D, Murti YB, Pratiwi SUT, Sudarsono S, Hanna G, Hamann MT. TLC-based fingerprinting analysis of the geographical variation of Melastoma malabathricum in inland and archipelago regions: A rapid and easy-to-use tool for field metabolomics studies. J Nat Prod. 2022; 85(1):292–300. https://doi.org/10.1021/acs.jnatprod.1c00622
20.Aderibigbe SA, Anowai OC. An investigation of the effect of seasonal variation on the phytochemical constituents in two Nauclea species. Trop J Nat Prod Res. 2020; 4(2):172–178. https://doi.org/10.26538/tjnpr/v4i2.15
21.Mayo DW, Miller FA, Hannah RW. Course Notes on the Interpretation of Infrared and Raman Spectra. Hoboken, NJ: John Wiley & Sons; 2004. p. 1–462.
22.Jeffery GH, Bassett J, Mendham J, Denney RC. Vogel’s Textbook of Quantitative Chemical Analysis. 5th ed. London: Longman; 1989. p. 744.