Ameliorative Effects of Ethyl acetate Fraction of Millettia aboensis Stem Bark on Loperamide-Induced Constipation in Rats
doi.org/10.26538/tjnpr/v4i8.21
Keywords:
Loperamide, Constipation, Laxative effect, Phytochemicals, Millettia aboensisAbstract
Millettia aboensisis is one of the plants that have been used for the treatment of different diseases such as gastrointestinal disturbances, ulcer and venereal diseases in Nigeria folklore medicine. This study evaluated the phytochemistry and effects of the ethyl acetate fraction of Millettia aboensis stem bark on loperamide-induced constipation in rats. The stem bark of Millettia aboensis was first extracted with methanol and further partitioned using aqueous methanol (20%), ethyl acetate and n-hexane. The ethyl acetate fraction was then used for the study. The experimental rats employed for this study were randomly divided into six groups of four rats each. Group 1 (normal control) received distilled water; Group 2 (positive control) received 3 mg/kg b.w. Loperamide, while group 3 received 5 mL/kg b.w. bisacodyl. Groups 4-6 received the graded doses of the fraction (100, 300, and 600 mg/kg b.w). After 7 days of
standard drug and Millettia aboensis fraction administration, groups 2 to 6 received 3 mg/kg b.w. loperamide. The result of the phytochemical screening of the ethyl acetate fraction of Millettia aboensis stem bark revealed the presence of phenols, flavonoids, terpenoids, saponins, alkaloids, glycosides and tannins.The results of water intake; number of faecal pellets output and faecal weight showed significant increase (p < 0.05), while the body weight of the rats decreased significantly (p < 0.05) when compared with the control groups. The results of the study showed that Millettia aboensis ethyl acetate fraction exerted a mild laxative effect against loperamideinduced constipation in rats.
References
Chatoor DE. Constipation and evacuation disorders. Best Prac Res Clin Gastroenterol. 2009; 23(4):517-530.
Cirillo C and Capasso R. Constipation and botanical medicines: an overview. Phytother Res. 2015; 29:1488 - 1493.
Drossman DA. Functional Gastrointestinal Disorders: History, Pathophysiology, Clinical Features, and Rome IV. Gastroenterol. 2016; 150:1262 – 1279.
Leung L, Riutta T, Kotecha J, Rosser W. Chronic constipation: an evidence-based review. J Am Board Fam Med. 2011; 24(4):436 - 451.
Mostafa SM, Bhandari S, Ritchie G, Gratton N, Weastone R. Constipation and its implication in the critically ill patient. Br J Anaesthesiol. 2003; 91:815 - 819.
Ncube NS, Afolayan AJ, Okoh AJ. Assessment techniques of antimicrobial properties of natural compounds of plants origin: current methods and future trends. Afr J Biotechnol. 2008; 7(12):1797-1806.
Egba IS, Ogbodo JO, Ogbodo PO, Obike CA. Toxicological Evaluation of Two Named Herbal Remedies Sold Across Orumba South Local Government Area of Anambra State, South-Eastern Nigeria. Asian J Biochem. 2018;1(1):1–6.
Afolabi OA, Aderoju AH, Alagbonsi AI. Effects of methanolic extract of Moringaoleifera leaves on semen and biochemical parameters in cryptorcid rats. Afr J Trad Comp Altern Med. 2013; 10(5):230-235.
Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol. 2005; 4(7):685-688.
Kaiko GE, Ryu SH, Koues OI, Collins PL, Solnica-Krezel L, Pearce JE, Pearce EL, Oltz ME, Stappenbeck ST. The colonic crypt protects stem cells from microbiota-derived metabolites. Cell. 2016; 165(7):1708-1720.
Okwuonu CG, Chukwuonye IJ, Adejumo OA, Agaba EI, Ojogwu LI. Prevalence of chronic kidney disease and its risk factors among adults in a semi-urban community of South-East Nigeria. Niger Postgrad Med J. 2017; 24:81-87.
Madziga HA, Sanni S, Sandabe UK. Phytochemical and elemental analysis of Acalphawilkesiana leaf. J Am Sci. 2010; 69(11):510-514.
Mosihuzzaman M. Herbal medicine in health care. An overview. Nat Prod Crop Protect. 2012; 29:913-920.
Ugwueze ME, AdonuCC, Attama AA. Evaluation of the hepatoprotective activity of root extracts of Millettia aboensis on carbon tetrachloride [CCl4] induced hepatotoxicity in rats. Int J Adv Res. 2013; 1(5):65 - 70.
Trease GE and Evans WC. Trease and Evans Pharmacognosy. 4thEdn. W. B. Saunders, USA, 2002. 820 – 835 p.
Harbone JB. Phytochemicals methods: a guide to modern techniques of plant analysis. Chapman and Hall Ltd., London, 1998. 49 – 188 p.
Soni A and Sosa S. “Phytochemical Analysis and Free Radical Scavenging Potential of Herbal and Medicinal Plant Extracts.” J Pharm Phytochem. 2013; 2(4):22-29.
Wintola OA, Sunmonu TO, Afolayan AJ. The effect of Aloeferox mill in treatment of loperamide-induced constipation in Wistar rats. BMC Gastroenterol. 2010; 10:95.
Mikhail ON, Taoheed AA, Rukayat OJ, Mutiu IK. Ameliorative effect of Lecaniodiscus cupanioides (Sapindaceae) Aqueous Root Extract in Loperamideinduced Constipated Rats. Trop J Pharm Res. 2015; 14(6):1057-1062.
Izzo AA, Nicoletti M, Giannattasio B, Capasso F. Antidiarrheal Activity of Terminalia Sericea Burch. ex DC Extracts. Nat Drugs Dig Tract, Capasso, F Mascolo, N (Eds), EMSI, Rome. 1992. 225-256 p.
Ezike AC, Akah PA, Okoli CO, Ufere IK, Ezeudu E, Okoye CF, Ashara C, Igbokwe IN. “Studies on Gastrointestinal Effects of Desmodiumvelutinum: A Traditional Remedy for Diarrhea.” Am J Pharm Toxicol. 2014; 9(2):114-124.
Kim JE, Lee MR, Park JJ, Choi JY, Song BR, Son HJ, Choi YW, Kim KM, Hong JT, Hwang DY. Quercetin have been reported to possess laxative effect by promoting gastrointestinal motility and mucin secretion in loperamideinduced constipation of SD rats through regulation of the
mAChRs downstream signal. Pharm Biol. 2018; 56(1):309- 317.
Njoku UO, Uzoagulu CH, Ogugofor MO. Protective effect of Cnidoscolus aconitifoilus leaves against diclofenacinduced gastric mucosal damage. Pak J Pharm Sci. 2020; 33(2):651-657.
Panche AN, Diwan AD, Chandra SR. Flavonoids: An overview J NutrSci, 2016; 5:e47-e55.
Njoku UO, Nwodo OFC,Ogugofor MO. Cardioprotective potential of methanol extract of Costusafer leaf on carbontetrachloride-induced cardiotoxicity in albino rats. Asian J Pharm Res Health Care 2017; 9(2):51-58.
Petridis GK. Tannins: Types, Foods Containing and Nutrition. Nova Science publishers, New York, 2010. 231 – 247 p.
Gaikwad SB, Mohan GK, Rani MS. Phytochemicals for diabetes management. Pharm Crop. 2014; 5(1):11 - 28.
Hanauer SB. The role of loperamide in gastrointestinal disorders. Reviews in GastroenterolDisord. 2008; 8(1):15 – 20.
Lee HY, Kim JH, Jeung HW. Effects of Ficuscarica Paste on Loperamide-Induced Constipation in rats. Food ChemToxicol. 2012; 50:895 – 902.
Méité S, Bahi C, Yéo D, Datté JY, Djaman JA, N'guessan DJ. Laxative activities of Mareyamicrantha (Benth.) Müll. Arg. (Euphorbiaceae) leaf aqueous extract in rats. BMC CompAltern Med. 2010;10:7.
Eor JY, Tan PL, Lim SM, Choi DH, Yoon SM, Yang SY, Kim, HS. Laxative effect of probiotic chocolate on loperamide-induced constipation in rats. Food Res Int. 2019; 116:1173-1182.
Marjorie C. Plant products as antimicrobial agents. ClinMicrobiol Rev. 1996; 12:564 - 582.
Niwa T, Nakao M, Hoshi S, Yamada K, Inagaki K, Nishida M, Nabeshima T. Effect of dietary fiber on morphineinduced constipation in rats. Biotech Biochem. 2002; 66(6):1233 - 1240.
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