UPLC-MS Profile and Anti-Proliferative Activity of the Berries of an Aggressive Wild-Growing Weed: Solanum elaeagnifolium Cav. (Solanaceae)



  • Khawla D. Al-Hamaideh Department of Basic Medical Sciences, Faculty of Medicine, Al-Balqa Applied University, Al-Salt 19117, Jordan
  • Isra Dmour Department of pharmacy, Faculty of Pharmacy, Zarqa University, Zarqa, Jordan
  • Tamam El-Elimat Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan
  • Fatma U. Afifi Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmacy, Applied Science University, Amman, Jordan


Colorectal cancer, Jordan, Obesity, Solanum elaeagnifolium Cav, Solanaceae, UPLC-MS


Although, Solanum elaeagnifolium Cav. is described as a problematic weed difficult to eradicate, it is used in the traditional medicine of different countries for the treatment of different ailments, such as tooth ache and constipation. Its occurrence in Jordan is widespread. In the present study, the aim was to identify the phytochemical composition of the berries and to screen
for different biological activities. Phytochemically, the hydro-alcoholic extracts of the berries of S. elaeagnifolium were analyzed using Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). Pharmacologically, using Sulforhodamine B (SRB) assay, the antiproliferative effect of the extracts against four colorectal cancer cell lines (HT29, HCT116, SW620 and CACO2) was tested. The aqueous extracts of the ripe and unripe berries were further evaluated for their in vitro anti-lipidemic and anti-obesity activities. UPLC-MS analysis resulted in the identification of seven bioactive compounds in both extracts. Three of them,
namely; apigenin, hyperoside and luteolin were identified in these berries for the first time. Also,chlorogenic acid, kaempferol, solasodine and solamargine were identified. The extractshibited anti-proliferative activity but lacked anti-obesity activity in vitro. This is the first report on the phytochemical and pharmacological evaluation of the ripe and unripe berries of S. elaeagnifolium Cav., growing wild in Jordan. 

Author Biography

Fatma U. Afifi, Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmacy, Applied Science University, Amman, Jordan

Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman, Jordan


Taha H, Jaghbeer MA, Shteiwi M, Alkhaldi S, Berggren V. Knowledge and Perceptions about Colorectal Cancer in Jordan. Asian Pac J Cancer Prev. 2016; 16(18):8479-8486.

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, and Bray F. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2015; 136(5):E359-E386.

Benarba B and Pandiella A. Colorectal cancer and medicinal plants: Principle findings from recent studies. Biomed Pharmacother. 2018; 107:408-423.

Abu-Rish EY, Kasabri V, Hudaib MM, Mashalla SH, Alalawi LH, Tawaha K, Mohammad MK, Mohamed YS, Bustanji Y. Evaluation of antiproliferative activity of some traditional anticancer herbal remedies from Jordan. Trop J Pharm Res. 2016; 15(3):469-474.

Al-Tawarah NM, Qaralleh H, Khlaifat AM, Nofal MN, Alqaraleh M, Khleifat KM, Al-Limoun MO, and Al Shhab MA. Anticancer and Antibacterial Properties of Varthemia iphionides Essential Oil/Silver Nanoparticles. Biomed Pharmacol J. 2020; 13(3):1175-1184.

Kasabri V, Afifi FU, Abu-Dahab R, Mashallah S. Mitigating Efficacy of Inula graveolens (L.) Desf.(Asteraceae) In Breast Adenocarcinoma MCF7 and T47D Proliferation: In Vitro Mechanistic Studies of A Selected Ethnomedicinal Plant from Jordan. Rom Biotechnol Lett. 2017; 22(6):13044.

Jochem C and Leitzmann M. Obesity and Colorectal Cancer. Recent Results Cancer Res. 2016; 208:17-41.

Seetaloo AD, Aumeeruddy MZ, Rengasamy Kannan RR, Mahomoodally MF. Potential of traditionally consumed medicinal herbs, spices, and food plants to inhibit key digestive enzymes geared towards diabetes mellitus management — A systematic review. S Afr J Bot. 2019;


Afifi FU, Kasabri V, Litescu S, Abaza IF, Tawaha K. Phytochemical and biological evaluations of Arum hygrophilum Boiss. (Araceae). Pharmacogn Mag. 2017; 13(50):275-280.

Al-Hamaideh KD, El-Elimat T, Afifi FU, Kasabri V. Phytochemical Screening and Pharmacological Activities of Echium judaeum Lacaita Extracts Growing Wild in Jordan. J Pharm Sci. 2017; 10(3):153-164.

Haddad MA, Dmour H, Al-Khazaleh JFM, Obeidat M, AlAbbadi A, Al-Shadaideh AN, Al-Mazra'awi MS, Shatnawi MA, Iommi C. Herbs and Medicinal Plants in Jordan. J AOAC Int. 2020; 103(4):925-929.

Al-Eisawi D. Flora of Jordan Checklist, Revised; The University of Jordan Press; 2013. 168 p.

Brunel S. Pest risk analysis for Solanum elaeagnifolium and international management measures proposed. EPPO Bull. 2011; 41(2):232-242.

Camazine S and Bye RA. A study of the medical ethnobotany of the Zuni Indians of New Mexico. J Ethnopharmacol. 1980; 2(4):365-388.

Badawy A, Zayed R, Ahmed S, Hassanean H. Phytochemical and pharmacological studies of Solanum elaeagnifolium growing in Egypt. J Nat Prod India. 2013; 6:156-167.

Lee K-R, Kozukue N, Han J-S, Park J-H, Chang E-Y, Baek E-J, Chang J-S, Friedman M. Glycoalkaloids and Metabolites Inhibit the Growth of Human Colon (HT29) and Liver (HepG2) Cancer Cells. J Agric Food Chem. 2004; 52(10):2832-2839.

Kaunda JS and Zhang Y-J. The genus Solanum: an ethnopharmacological, phytochemical and biological properties review. Nat prod bioprospect. 2019; 9(2):77- 137.

Zhu XC, Wu HW, Stanton R, Burrows GE, Lemerle D,Raman H. Morphological variation of Solanum elaeagnifolium in south-eastern Australia. Weed Res. 2013; 53(5):344-354.

Zhu XC, Wu HW, Raman H, Lemerle D, Stanton R,Burrows GE. Genetic variation and structure of Solanumelaeagnifoliumin Australia analysed by amplified fragment length polymorphism markers. Weed Res. 2013; 53(5):337-343.

Qasem JR, Al Abdallat AM, Hasan SM. Genetic diversity of Solanum elaeagnifolium, an invasive problematic weed in Jordan. Weed Res. 2019; 59(3):222-234.

El-Elimat T, Figueroa M, Ehrmann BM, Cech NB, Pearce CJ, Oberlies NH. High-Resolution MS, MS/MS, and UV Database of Fungal Secondary Metabolites as a Dereplication Protocol for Bioactive Natural Products. J Nat Prod. 2013; 76(9):1709-1716.

Chiale CA, Cabrera JL, Juliani HR. Kaempferol 3-(6″-ciscinnamoylglucoside) from Solanum elaeagnifolium. Phytochemistry. 1991; 30(3):1042-1043.

Kulkarni M and Pendse G. Glycoalkaloid contents in leaf, stem and root of Solanum elaeagnifolium. Planta Med. 1974; 25(03):249-252.

Tariq A, Sadia S, Pan K, Ullah I, Mussarat S, Sun F, Abiodun OO, Batbaatar A, Li Z, Song D, Xiong Q, Ullah R, Khan S, Basnet BB, Kumar , Islam R, Adnan M. A systematic review on ethnomedicines of anti-cancer plants. Phytother Res. 2017; 31(2):202-264.

Sergent T, Vanderstraeten J, Winand J, Beguin P, Schneider Y-J. Phenolic compounds and plant extracts as potential natural anti-obesity substances. Food Chem. 2012; 135(1):68-73.

Xavier CP, Lima CF, Preto A, Seruca R, FernandesFerreira M, Pereira-Wilson C. Luteolin, quercetin and ursolic acid are potent inhibitors of proliferation and inducers of apoptosis in both KRAS and BRAF mutated human colorectal cancer cells. Cancer Lett. 2009; 281(2):162-170.

Houda M, Derbre S, Jedy A, Tlili N, Legault J, Richomme P, Limam F, Saidani-Tounsi M. Combined anti-ages and antioxidant activities of different solvent extracts of Solanum elaeagnifolium Cav (Solanaceae) fruits during ripening and related to their phytochemical compositions. EXCLI J. 2014; 13:1029-1042.

Ku SK, Sung SH, Choung JJ, Choi J-S, Shin YK, Kim JW. Anti-obesity and anti-diabetic effects of a standardized potato extract in ob/ob mice. Exp Ther Med. 2016; 12(1):354-364.

Mahomoodally FM and Ramcharun S. In vitro kinetics of inhibition of lipase, antioxidant activity, glucose entrapment and polyphenolic content of Solanum linnaeanum. J Biol Act Prod Nat. 2015; 5(6):383-396.

Hossain SJ, El-Sayed MA, Mohamed A-HH, Sheded MG, Aoshima H. Phenolic content, anti-oxidative, anti-alphaamylase and anti-alpha-glucosidase activities of Solanum diphyllum. Bangladesh J Bio. 2009; 38(2):139-143.

Orqueda ME, Rivas M, Zampini IC, Alberto MR, Torres S, Cuello S, Sayago J, Thomas-Valdes S, Jimenez-Aspee F, Schmeda-Hirschmann G, Isla MI. Chemical and functional characterization of seed, pulp and skin powder from chilto (Solanum betaceum), an Argentine native fruit. Phenolic fractions affect key enzymes involved in metabolic syndrome and oxidative stress. Food Chem. 2017; 216:70-79.




How to Cite

Al-Hamaideh, K. D., Dmour, I., El-Elimat, T., & Afifi, F. U. (2020). UPLC-MS Profile and Anti-Proliferative Activity of the Berries of an Aggressive Wild-Growing Weed: Solanum elaeagnifolium Cav. (Solanaceae): doi.org/10.26538/tjnpr/v4i12.16. Tropical Journal of Natural Product Research (TJNPR), 4(12), 1131–1138. Retrieved from https://www.tjnpr.org/index.php/home/article/view/891