Total Phenolic, Total Flavonoid Contents, and Radical Acavenging Activity of Different Concentrations of Ethanol Extract of Pyrrosia piloselloides (L) M. G. Price
doi.org/10.26538/tjnpr/v6i4.5
Keywords:
Antioxidant, DPPH, Flavonoid, Phenolic, Pyrrosia piloselloidesAbstract
Pyrrosia piloselloides (L.) M.G. Price (PP) is known to have pharmacological activities. This study investigated the total phenolic (TPC), total flavonoid contents (TFC), and the radical scavenging activities (RSA) of different concentrations of PP ethanol extract. PP dried sample was macerated with different ethanol concentrations (30%, 50%, and 70%). The TPC and TFC were determined using Folin-Ciocalteu and AlCl3 calorimetric assays, respectively while the RSA was investigated using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. It was discovered that TPC (9.74 mg GAE/g), TFC (6.21 mg QE/g), and RSA (73.50%) of 50% ethanol extract of PP were higher than extracts obtained with 30% and 70% ethanol. Furthermore, a positive correlation was found between RSA vs TPC (r=0.43) and RSA vs TFC (r=0.35). It was concluded that 50% ethanol could be the best ethanol concentration to obtain PP extract with high contents of TPC, TFC, and antioxidant properties.
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