Effect of Selected Pure Compounds of Plant Origin on the Proliferation, Adhesion, Migration, and Invasion of Human Prostate Cancer Cells
doi.org/10.26538/tjnpr/v5i2.7
Keywords:
Antiproliferative activity, Medicinal plants, Natural Products, Prostate cancerAbstract
Signalling cascade of autocrine/paracrine growth factors plays an important role in promoting prostate cancer (PCa) cells growth, survival, migration, invasion, and progression. PCa is one of the most important causes of death in men, thus new therapeutic approaches are needed. The objective of this study is to investigate whether α-terpinene, -caryophyllene, linalool, luteolin, and quercetin exert antiproliferative, anti-adhesion, anti-migratory, and anti-invasion activity in PCa cell line models. The results showed that all five compounds exhibited growth inhibitory activities on both PCa cell lines (PC3 and DU145). Luteolin was the most potent antiproliferative compound against PC3 and DU145 cells with IC50 values of 70.19 µM and 37.46 µM, respectively. A number of techniques, including cell adhesion, wound healing, Transwell migration and invasion assays were used to investigate the ability of these compounds to inhibit migration/invasion of PCa cells at non-toxic concentrations. Our findings highlight the potential of luteolin and quercetin to inhibit the invasion and migration of PC3 and DU145 cells without causing cytotoxicity. However, further studies are still needed to reveal their molecular mechanisms of action
References
Shrivastava A and Gupta VB. Various treatment options for benign prostatic hyperplasia: A current update. J mid-life health. 2012; 3(1):10-19.
Society AC: Key Statistics for Prostate Cancer. 2018.
Siegel RL, Miller KD, Fedewa SA, Ahnen DJ, Meester RGS, Barzi A, Jemal A. Colorectal cancer statistics, 2017. CA Cancer J
Clin. 2017; 67(3):177-193.
Abdel-Razeq H, Attiga F, Mansour A: Cancer care in Jordan. Hematol Oncol Stem Cell Ther. 2015; 8(2):64-70.
Revelo MP, Cookson MS, Chang SS, Shook MF, Smith JA, Jr., Shappell SB. Incidence and location of prostate and urothelial carcinoma in prostates from cystoprostatectomies: implications for possible apical sparing surgery. J Urol. 2004; 171(2 Pt 1):646-651.
Bubendorf L, Schopfer A, Wagner U, Sauter G, Moch H, Willi N, Gasser TC, Mihatsch MJ. Metastatic patterns of prostate cancer: an autopsy study of 1,589 patients. Hum Pathol. 2000; 31(5):578-583.
Shu YZ: Recent natural products based drug development: a pharmaceutical industry perspective. J Nat Prod 1998; 61(8):1053-1071.
Baldissera MD, Da Silva AS, Oliveira CB, Santos RC, Vaucher RA, Raffin RP, Gomes P, Dambros MG, Miletti LC, Boligon AA. Trypanocidal action of tea tree oil (Melaleuca alternifolia) against Trypanosoma evansi in vitro and in vivo used mice as experimental model. Exp Parasitol. 2014; 141:21-27.
Aviram M and Fuhrman B. Wine flavonoids protect against LDL oxidation and atherosclerosis. Annals of the New York Acad of Sci. 2002; 957(1):146-161.
Abu Dahab RA and Afifi-Yazar F. Comparison of antiproliferative activities of ethanolic plant extracts of the
Jordanian flora using MCF7 and A549 cells. Planta Med. 2007;73(09):535.
Afifi F, Abu-Dahab R, Kasabri V, Abaza I. GC-MS composition and antiproliferative activity of Inula graveolens (L.) Desf. essential oil. Arab J Med Arom Plants. 2015; 1(1):57-66.
Afifi FU, Kasabri V, Beltran S, Abuhammad A, Abaza IF, Ganado O, Al-Gabbiesh AH. Comparison of different methods in determination of essential oil composition of Origaum syriacum from Jordan and its modulation of pancreatic enzymes. Rev Roum Chim. 2017; 62(1):15-21.
Wu X, Zeng H, Zhang X, Zhao Y, Sha H, Ge X, Zhang M, Gao X, Xu Q. Phosphatase of regenerating liver-3 promotes motility and metastasis of mouse melanoma cells. The Am J Pathol. 2004;164(6):2039-2054.
Coté H, Boucher M-A, Pichette A, Legault J. Anti-inflammatory, antioxidant, antibiotic, and cytotoxic activities of Tanacetum vulgare L. essential oil and its constituents. Med. 2017; 4(2):34.
Amiel E, Ofir R, Dudai N, Soloway E, Rabinsky T, Rachmilevitch S. β-Caryophyllene, a compound isolated from the biblical balm of gilead (Commiphora gileadensis), is a selective apoptosis inducer for tumor cell lines. Evid-Based Compl Altern Med: eCAM. 2012; 2012:872394.
El Hadri A, del Rio MG, Sanz J, Coloma AG, Idaomar M, Ozonas BR, González JB, Reus MIS. Cytotoxic activity of α-humulene and transcaryophyllene from Salvia officinalis in animal and human tumor cells. An R Acad Nac Farm. 2010; 76(3):343-356.
Chang M-Y and Shen Y-L. Linalool exhibits cytotoxic effects by activating antitumor immunity. Molecules. 2014; 19(5):6694-6706.
Zhao Y, Chen R, Wang Y, Qing C, Wang W, Yang Y. In vitro and in vivo efficacy studies of Lavender angustifolia essential oil and its active constituents on the proliferation of human prostate cancer. Integr Cancer Ther. 2017; 16(2):215-226.
Horinaka M, Yoshida T, Shiraishi T, Nakata S, Wakada M, Nakanishi R, Nishino H, Matsui H, Sakai T. Luteolin induces
apoptosis via death receptor 5 upregulation in human maligant tumor cells. Oncogenes. 2005; 24(48):7180-189.
Fang J, Zhou Q, Shi X-l, Jiang B-h. Luteolin inhibits insulin-like growth factor 1 receptor signaling in prostate cancer cells. Carcinogen. 2007; 28(3):713-723.
Yang F, Song L, Wang H, Wang J, Xu Z, Xing N. Quercetin in prostate cancer: Chemotherapeutic and chemopreventive effects, mechanisms and clinical application potential. Oncol Rep. 2015;33(6):2659-2668.
Seo Y, Ryu K, Park J, Jeon D-k, Jo S, Lee HK, Namkung W. Inhibition of ANO1 by luteolin and its cytotoxicity in human
prostate cancer PC-3 cells. PloS one. 2017; 12(3):e0174935.
Senthilkumar K, Arunkumar R, Elumalai P, Sharmila G, Gunadharini DN, Banudevi S, Krishnamoorthy G, Benson CS,
Arunakaran J. Quercetin inhibits invasion, migration and signalling molecules involved in cell survival and proliferation of prostate cancer cell line (PC-3). Cell Biochem Funct. 2011; 29(2):87-95.
Zhou Q, Yan B, Hu X, Li X-B, Zhang J, Fang J. Luteolin inhibits invasion of prostate cancer PC3 cells through E-cadherin. Mol Cancer Ther. 2009; 8(6):1684-1691.
Dunsmuir W, Gillett C, Meyer L, Young M, Corbishley C, Eeles R, Kirby R. Molecular markers for predicting prostate cancer stage and survival. BJU Int. 2000; 86(7):869-878.
Richmond PJ, Karayiannakis AJ, Nagafuchi A, Kaisary AV, Pignatelli M. Aberrant E-cadherin and α-catenin expression in prostate cancer: correlation with patient survival. Cancer Res. 1997; 57(15):3189-3193.
Pratheeshkumar P, Son Y-O, Budhraja A, Wang X, Ding S, Wang L, Hitron A, Lee J-C, Kim D, Divya SP. Luteolin inhibits human prostate tumor growth by suppressing vascular endothelial growth factor receptor 2-mediated angiogenesis. PloS one. 2012;7(12):e52279.
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