HPLC Fingerprint Associated with Chemometrics of Guiera senegalensis Leaf Extracts
doi.org/10.26538/tjnpr/v5i3.27
Keywords:
Guiera senegalensis, Phytochemicals, Fingerprint, RP-HPLCAbstract
Guiera senegalensis is a shrub reported to have several medicinal properties due to its vast phytochemicals. However, the concentration of these phytochemicals is affected by several factors such as location and season of harvest. The aim of this research is to establish a chromatographic fingerprint of the phytochemicals in G. senegalensis leaf samples collected from two states in Northwest Nigeria at two different seasons. HPLC method was adopted and validated for the fingerprint analysis of samples collected. Chemometric techniques such as percent relative standard deviation (% RSD) of the relative retention time (RRT) and relative peak area (RPA), similarity and difference analysis were conducted. The adopted HPLC method was found to be suitable for the analysis as the % RSD of both intraday and interday precisions were less than 3%. Fingerprint analysis showed excellent similarity between the samples as indicated by % RSD of RRT (< 1.6 %), degree of difference (0.00-0.014) and similarity (0.995- 1) values. The high % RSD values of RPA (17-120%) indicated variations in concentrations of the phytochemicals present in the G. senegalensis leaf samples. This method can therefore, be used for the routine quality control of G. senegalensis leaf sample.
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