Assessment of Acute and Sub-Chronic Toxicity of HYK Aqueous Extracts in Experimental Animals
doi.org/10.26538/tjnpr/v5i3.17
Keywords:
Traditional Vietnamese Medicine, HYK recipe, Premenopausal syndrome, ToxicityAbstract
Hoa Yên Kinh (HYK) is an oriental prescription in Vietnamese Traditional Medicine that is used to treat premenopausal syndrome in the clinical stage. This study aimed to evaluate the safety,acute and sub-chronic toxicity of aqueous extracts of HYK in in vivo model. In the acute toxicity study, HYK at doses ranging from 150 to 300 g /kg/day was given to Swiss mice by oral administration. Mice were observed for general behavioral changes, adverse effects, and mortality up to 7 days post-treatment to find the highest dose that did not kill mice. In sub-chronic toxicity studies, HYK was given orally with doses of 12 g and 60 g/kg/day for 12 weeks. At the end of 12 weeks, the mice were operated to observe the whole organ for histopathology. The microscopic structures of the liver, spleen, and kidney of at least 30% of mice in each group was randomly checked. The acute toxicity study in all the doses used did not cause any significant change such that no LD50 was found. In addition, the sub-chronic toxicity study did not show any treatment-related abnormalities in the hematological and biochemical parameters. These results demonstrated that HYK aqueous extract is safe and does not appear to exert toxic effect.
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