Curative Effect of Aqueous Seed Extract of Citrus paradisi against Carbon Tetrachloride-Induced Nephrotoxicity in Wistar Rats
doi.org/10.26538/tjnpr/v5i4.25
Keywords:
Nephrotoxicity, Carbon tetrachloride, Serum electrolytes, Toxicology, Citrus paradisiAbstract
Carbon tetrachloride (CCl4) is a possible nephrotoxin that leads to grievous and harmful chemical toxicity. C.paradisi contains phytochemicals,vitamins and flavonoids which possesses strong anti-inflammatory, and antioxidant properties. This study investigated the serum electrolytes levels and the efficacy of C. paradisi to ameliorate the effect of carbon tetrachloride-induced nephrotoxicity in Wistar rats. The aqueous seed extract of C. paradisi was administered at a dose of 125-500 mg/kg body weight. Thirty albino rats were assigned into 6 groups of 5 rats in each. Group I was the 1ml/kg normal saline while group II was intraperitoneally treated with 3 mL/kg of CCl4 1 hour before oral treatment (p.o.) with 10 mL/kg of normal saline while groups III-VI rats were intraperitoneally treated with 3 mL/kg 30% CCl4 1 hour before oral treatment with 10 mg/kg of ascorbic acid. Groups III-VI were administered with 125 mg/kg, 250 mg/kg, and 500 mg/kg of aqueous seed extract of C.paradisi. Results shows an increase in all the serum electrolytes except in serum phosphate as a result of contamination. The result insinuated that kidney damage was induced in the rats with ascorbic acid attenuating the effect of carbon tetrachloride at each dose. The treatment showed that aqueous seed extract of C. paradisi controls an increase in serum electrolytes.
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