Mechanisms Involved in the Antiulcer Activity of Capparis atamisquea Polar Leaves Extracts
Keywords:
Antiulcer activity, Atamisqui, Gastroprotective mechanisms, Polar extractsAbstract
Capparis atamisquea Kuntze (atamisqui) is used in northern Argentina to treat digestive disorders, however, scientific information on its potential therapeutic application has not been established. The study was designed to evaluate the gastroprotective effect and possible involvement mechanism of 5% infusion and 10% hydroalcoholic Capparis atamisquea leaf extracts in experimental model of acute gastric ulceration. Ethanol-induced gastric ulcer model was performed in adult Wistar rats followed by macroscopic and histological analysis, gastric mucus content quantification, mucosal antioxidant system study, myeloperoxidase activity determination and nitric oxide and prostaglandin E2 content. The in vitro antioxidant activities of both extracts were also determined. Pre-treatments with both extracts caused a decrease in ethanol-induced gastric injuries at a macroscopic and microscopic level. A significant increase in gastric mucus content was also observed. The level of nitric oxide and prostaglandin E2 in the gastric mucosa increased significantly in the pre-treatment groups compared to the ulcer control group. These results show the participation of these mediators in the gastroprotective effect. In addition, both extracts showed moderate in vivo and in vitro antioxidant activity. These results contribute to validate the use of this native plant which represents a natural alternative for gastric ulcer prevention.
References
Newman DJ and Cragg GM. Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. J Nat Prod. 2020; 83:770-803.
Boelcke O. Plantas vasculares de la Argentina nativas y exóticas. 2nd edn. (Hemisferio Sur, Buenos Aires, 1992.334p.
González DS and Gutiérrez MC. Plantas de los Salares del Chaco Arido. 1st edn. (Gutierrez DS, La Rioja, 2014. 45p.
Reynoso MA, Medina FA, Vera NR. Therapeutic potential of organic unifloral atamisqui honey (Atamisquea emarginata): in vitro and in vivo biological evaluation. Int J Mod Pharm Res. 2020; 4(4):221-230.
Nazar S, Hussain MA, Khan A, Muhammad G, Tahir MN. Capparis decidua Edgew (Forssk.): A comprehensive review of its traditional uses, phytochemistry, pharmacology and nutrapharmaceutical potential. Arab J Chem. 2020; 13(1):1901-1916.
Pelotto JP, Del Pero Martınez MA. ́ Flavonoid aglycones from Argentinian Capparis Species (Capparaceae). Biochem Syst Ecol. 1998; 26:577-80.
De Barros M, da Silva LM, Boeing T, Somensi LB, Cury BJ, Burci LM, Santin JR, Andrade SF, Monache FD, Cechinel-Filho V. Pharmacological reports about gastroprotective effects of methanolic extract from leaves of Solidago chilensis (Brazilian arnica) and its components quercitrin and afzelin in rodents, Naunyn Schmiedebergs Arch Pharmacol. 2016; 389(4):403-417.
Beber AP, de Souza P, Boeing T, Somensi LB, Mariano LNB, Cury BJ, Burci LM, da Silva CB, Simionatto E, de Andrade SF, da Silva LM. Constituents of leaves from Bauhinia curvula Benth. exert gastroprotective activity in rodents: role of quercitrin and kaempferol. Inflammopharmacol. 2018; 26(2):539-550.
Da Luz BB, De Oliveira AF, Maria Ferreira D, Dallazen JL, Cipriani TR, de Souza LM, Werner MFP, Chemical composition, antioxidant and gastrointestinal properties of Sedum dendroideum Moc & Sessé ex DC leaves tea infusion, J Ethnopharmacol. 2019; 1:141-151.
Lu H, Graham DY. New development in the mechanistic understanding of peptic ulcer diseases. Drug Discov Today Dis Mech. 2006; 3(4):431-437.
Sheen E and Triadafilopoulos G. Adverse effects of longterm proton pump inhibitor therapy, Dig Dis Sci. 2011; 6:931-950.
Farmacopea Argentina, 7th edn. 1050 Formas Farmacéuticas, 2003. Available from: http://www.anmat.gov.ar/webanmat/fna/pfds/Farmacopea_Argentina_2013_Ed.7.pdf Accessed 15 Dec 2021.
Taboada FF, Habib NC, Genta SB. Gastroprotective Effect of Acacia aroma Gill. ex Hook. and Arn. Leaves Extracts in Gastric Ulcer Models in Rats. Int J Pharm Investig. 2019;9(4):187-194.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958; 181:1199-200.
Kumar S, Yadav A, Yadav M, Yadav JP. Effect of climate change on phytochemical diversity, total phenolic content and in vitro antioxidant activity of Aloe vera (L.) Burm.f, BMC Res Notes. 2017; 10:60.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-275.
Bradley PP, Christensen RD, Rothstein G. Cellular and extracellular myeloperoxidase in pyogenic inflammation. Blood. 1982; 60(3):618-622.
Ellman GC. Tissue sulfhydryl groups. Arch Biochem Biophy. 1959; 82:70-77.
Beuge JA and Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978; 52:302-310.
Aebi H. Catalase in vitro. Methods Enzymol. 1984; 105:121-123.
Goshi E, Zhou G, He Q. Nitric oxide detection methods in vitro and in vivo. Med Gas Res. 2019; 9(4):192-207.
Breviglieri E, Mota da Silva L, Boeing T, Somensi LB, Cury BJ, Gimenez A, Cechinel Filho V, de Andrade SF. Gastroprotective and anti-secretory mechanisms of 2-phenylquinoline, an alkaloid isolated from Galipea longiflora. Phytomed. 2017; 25:61-70.
Francik S, Francik R, Sadowska U, Bystrowska B, Zawiślak A, Knapczyk A, Nzeyimana A. Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves. Materials (Basel). 2020; 13(24):5811.
Alvarez MA. Yungas. In: Pharmacological Properties of Native Plants from Argentina. Springer, Cham. 2019. 34p.
Hollander D, Tarnawski A. The protective and therapeutic mechanisms of sucralfate. Scand J Gastroenterol Suppl.1990; 173:1-5.
Mota KS, Dias GE, Pinto ME, Luiz-Ferreira A, Souza-Brito AR, Hiruma-Lima CA. Flavonoids with gastroprotective activity. Molecules. 2009; 14(3):979-1012.
Mousa AM, El-Sammad NM, Hassan SK, Madboli AENA, Hashim AN, Moustafa ES, Bakry SM, Elsayed EA. Antiulcerogenic effect of Cuphea ignea extract against ethanol-induced gastric ulcer in rats. BMC Compl Altern Med. 2019; 19:345.
Ochiai T, Soeda S, Ohno S, Tanaka H, Shoyama Y, Shimeno H. Crocin prevents the death of PC-12 cells through sphingomyelinase–ceramide signaling by increasing glutathione synthesis. Neurochem Int. 2004; 44(5):321-330.
Alirezaei M, Dezfoulian O, Neamati S, Rashidipour M, Tanideh N, Kheradmand A. Oleuropein prevents ethanolinduced gastric ulcers via elevation of antioxidant enzyme activities in rats. J Physiol Biochem. 2012; 68(4):583-592.
Zakaria ZA, Balan T, Mamat SS, Mohtarrudin N, The LK, Salleh MZ. Mechanisms of gastroprotection of methanol extract of Melastoma malabathricum Leaves, BMC Complement Altern Med. 2015; 15:135.
De Araújo ERD, Guerra GCB, Araújo DFS, de Araújo AA, Fernandes JM, de Araújo Júnior RF, da Silva VC, de Carvalho TG, Ferreira LS, Zucolotto SM. Gastroprotective and Antioxidant Activity of Kalanchoe brasiliensis and Kalanchoe pinnata Leaf Juices against Indomethacin and
Ethanol-Induced Gastric Lesions in Rats. Int J Mol Sci. 2018; 19(5):1265.
Lian YZ, Lin IH, Yang YC, Chao JC. Gastroprotective effect of Lycium barbarum polysaccharides and Cphycocyanin in rats with ethanol-induced gastric ulcer. Int J Biol Macromol. 2020 ;165:1519-1528.
Laine L, Takeuchi K, Tarnawski A. Gastric mucosal defense and cytoprotection: bench to bedside. Gastroenterol. 2008; 135:41-60.
Saremi K, Rad SK, Tayeby F, Abdulla MA, Karimian H, Majid NA. Gastroprotective activity of a novel Schiff base derived dibromo substituted compound against ethanolinduced acute gastric lesions in rats. BMC Pharmacol Toxicol. 2019; 20(1):13.
Goel RK, Maiti RN, Tavares IA. Role of endogenous eicosanoids in the antiulcer effect of Kaempferol. Fitoterapia. 1996; 6:548-552.
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