Effect of Methanol Leaf Extract of Pterocarpus santalidoides L’ Herit ex DC on the Antioxidant and Lipid Profile of Wistar Rats
doi.org/10.26538/tjnpr/v5i7.22
Keywords:
Spectrophotometer, Pterocarpus santalinoides, Malondialdehyde, Lipid profile, AntioxidantAbstract
The leaf of Pterocarpus santalinoides (Family: Fabaceae) is used in Nigerian folklore medicine to relieve symptoms of diabetes, treat diarrhea and facilitate wound healing. The aim of this study was to investigate the methanol leaf extract of Pterocarpus santalinoides (MEPS) for antioxidant and hypolipidemic potentials. Fifty-six (56) mature Wistar rats were assigned to four groups (n=14) and fed for 90 days with rat chow supplemented with varying concentrations of MEPS (2.5, 5.0 and 10.0 mg/kg feed). On the 30th, 60th and 90th days blood samples were obtained from the retro-orbital plexus of four rats randomly selected from each experimental group and subjected to antioxidant assay and lipid profile, following standard methods. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) spectrophotometric assay was adopted for in vitro antioxidant evaluation of MEPS. The level of malondialdehyde was significantly (p < 0.05) decreased while catalase and superoxide dismutase were increased in the MEPS-treated rats by the different concentrations of MEPS. In the DPPH assay, MEPS (400 µg/mL) caused significant (p < 0.05) antioxidant activity (95.13%), comparable to 94.97% evoked by same concentration of ascorbic acid used as the reference drug. These results show that the methanol extract of P. santalinoides possesses significant antioxidant and hypolipidemic potentials.
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