Antidiarrheal Activity of Aqueous Extract of Artemisia campestris L. subsp. Glutinosa
doi.org/10.26538/tjnpr/v5i7.14
Keywords:
Antidiarrheal, Artemisia campestris L., Jejunum, MiceAbstract
Artemisia campestris L. subsp. glutinosa is used as a remedy for diarrhea in Moroccan folk medicine but has not been investigated for its antidiarrheal property. The aim of the present study was to evaluate the antidiarrheal activity of the aqueous extract of this plant, in order to verify it scientifically. The extract was prepared by infusion of the aerial part of the plant. The antidiarrheal activity was investigated using two standard methods, castor oil-induced diarrhoea, and gastrointestinal motility in mice. In addition, the antispasmodic activity was determined in vitro on the isolated rat jejunum mounted in an isotonic transducer. The extract caused a dose-dependent relaxation of the jejunum tone of rat pre-contracted by KCl (25 mM) with an IC50 of 1.20 ± 0.20 mg/mL. Oral pretreatment of mice with the extract at 100, 200, and 400 mg/kg,bw, delayed the onset of diarrhea, reduced the total number of defecations during 4 h, and decreased the total number of wet faeces in 4 h by 5.08, 18.64, and 38.98%, espectively. The results suggest that A. campestris L. has antidiarrheal activity and confirms its traditional use.
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