The Protective Effect of Kratom (Mitragyna speciosa Korth.) Leaves Extract on  Pancreas of Mice Exposed to Alcoholic Drinks: doi.org/10.26538/tjnpr/v5i7.11

Ruqiah G. P. Panjaitan; Ayu Kartika; Anita R. P. Raharjeng

Authors

Ruqiah G. P. Panjaitan Department of Biology Education, Faculty Teacher Training and Education, Tanjungpura University, Pontianak, West Kalimantan 78124, Indonesia
Ayu Kartika Department of Biology Education, Faculty Teacher Training and Education, Tanjungpura University, Pontianak, West Kalimantan 78124, Indonesia
Anita R. P. Raharjeng Biology Department, Faculty of Science and Technology, UIN Raden Fatah Palembang, South Sumatra 30126, Indonesia

Keywords:

Blood glucose, Histopathological, Mitragyna speciosa, Pancreas

Abstract

Consuming alcohol can damage the pancreas, and Mitragyna speciosa Korth. (kratom) leaves can be considered as stamina boosters. The study investigated the protective effect of Mitragyna speciosa leaves extract on the pancreas of mice exposed to alcoholic drinks (tuak). Twenty-four mice were randomly divided into six groups: A1, B1, and C1 received distilled water (0.50mL/20 g BW), M. speciosa leaves extract (0.29 mg/20 g BW), and a combination of silymarin and curcumin (0.70 mg/20 g BW), respectively, from day 1 to 8 and then received tuak (0.26mL/20 g BW) on day 8-14. The other three groups, A2, B2, and C2, received tuak (0.26 mL/20g BW) on day 1 to 8, then continued to receive distilled water (0.50 mL/20 g BW) (A2), M. speciosa leaves extract (0.29 mg/20 g BW) (B2), and the combination of silymarin and curcumin (0.70 mg/20 g BW) (C2) on day 8-14. The blood collection and pancreatectomy were performed on day 15. The blood glucose levels in A1 and A2 groups were higher than other groups (P<0.05). This group possessed high scores of pancreas damage such as inflammation and necrosis. The blood glucose levels of the M. speciosa leaves treatment were not significantly different (P>0.05) compared to the combination of silymarin and curcumin. Based on the histopathological analysis, M. speciosa demonstrated a protective effect on the pancreas compared to the combination of silymarin and curcumin group. The study suggested that M. speciosa leaves extract has the potency to protect the pancreas of alcohol-exposed mice.

References

Johnson J, Yanti Y, Suhartono MT, Widiati B. Isolation and purification of a fibrinolytic enzyme from bacterial strain isolated from tuak, an Indonesian palm wine. Int J Pharm Bio Sci. 2015; 6:988-996.

Riskiyani S, Jannah M, Rahman A. The Social Culture Aspect of Alcohol (Tuak) Used in North Toraja. Media Kesehatatan Indonesia Universitas Hasanuddin. 2015; 11:76-85.

Osna NA, Donohue TM, Kharbanda KK. Alcoholic liver disease: pathogenesis and current management. Alcohol Res. 2017; 38:147-161.

Rachdaoui N and Sarkar DK. Pathophysiology of the effects of alcohol abuse on the endocrine system. Alcohol Res. 2017; 38:255-276.

Noradina N, Hutagaol AN, Siregar Y. Pemberian vitamin E terhadap fragilitas eritrosit pada mencit (Mus musculus L.) yang dipapari tuak. J Ilmiah Keperawatan Imelda. 2017; 3:361-369.

Pandol SJ, Gorelick FS, Gerloff A, Lugea A. Alcohol abuse, endoplasmic reticulum stress and pancreatitis. Dig Dis. 2010;

:776-782.

Adnyane KM, Macdonald AA, Winarto A, Agungpriyono S. Studi mikroanatomi pankreas babirusa (Babyrousa babyrussa) menggunakan metode pewarnaan baku dan imunohistokimia. JKedokteran Hewan. 2010; 4:49-52.

Banjarnahor E and Wangko S. Sel beta pankreas sintesis dan sekresi insulin. Jurnal Biomedik. 2012; 4:156-162.

Dewi NP, Afifah AS, Tandi J, Yusriadi Y. Efek ekstrak etanol daun jambu air (Syzigium aqueum (Burm.f. Alston) terhadap histopatologi pankreas tikus putih. Farmakologika J Farmasi. 2018; 15:19-26.

Udeh NE, Anaga AO, Asuzu IU. Fasting blood sugar and clinical biochemistry profiles of diabetic rats treated with methanol leaf extract of Gnetum africanum Welw. Indian J Tradit Knowl. 2018; 7:445-450.

Toharin SNR, Cahyati WH, Zainafree I. Hubungan modifikasi gaya hidup dan kepatuhan konsumsi obat antidiabetik dengan kadar gula darah pada penderita diabetes melitus tipe 2 di RS Qim Batang tahun 2013. Unnes J Public Hlth. 2015; 4:153-161.

Jurnalis YD, Sayoeti Y, Elfitrimelly E. Peran antioksidan pada non-alcoholic fatty liver disease (NAFLD). Jurnal Kesehatatan Andalas. 2014; 3:15-20.

Setiawan VW, Pandol SJ, Porcel J, Wilkens LR, Le Marchand L, Pike MC, Monroe KR. Prospective Study of Alcohol Drinking, Smoking, and Pancreatitis: The Multiethnic Cohort. Pancreas. 2016; 45:819-825.

Peter Guengerich F and Avadhani NG. Roles of Cytochrome P450 in Metabolism of Ethanol and Carcinogens. Adv Exp Med

Biol. 2018; 1032:15-35.

Dinis-Oliveira RJ. Oxidative and Non-Oxidative Metabolomics of Ethanol. Curr Drug Metab. 2016; 17:327-335.

Warner ML, Kaufman NC, Grundmann O. The pharmacology and toxicology of kratom: from traditional herb to drug of abuse. Int J Legal Med. 2016; 130:127-138.

Gong F, Gu H, Xu Q, Kang W. Genus Mitragyna: ethnomedicinal uses and pharmacological studies. Phytopharmacol. 2012; 3:263-272.

Adkins JE, Boyer EW, McCurdy CR. Mitragyna speciosa, a psychoactive tree from Southeast Asia with opioid activity. Curr

Top Med Chem. 2011; 1:1165-1175.

Prozialeck WC, Jivan JK, Andurkar SV. Pharmacology of kratom: an emerging botanical agent with stimulant, analgesic and opioid-like effects. J Am Osteopath Assoc. 2012; 112:792-799.

Idayu NF, Hidayat MT, Moklas MA, Sharida F, Raudzah AR, Shamima AR, Apryani E. Antidepressant-like effect of mitragynine isolated from Mitragyna speciosa Korth in mice model of depression. Phytomedicine. 2011; 18:402-407.

Meireles V, Rosado T, Barroso M, Soares S, Gonçalves J, Luís Â, Caramelo D, Simão AY, Fernández N, Duarte AP, Gallardo E. Mitragyna speciosa: Clinical, Toxicological Aspects and Analysis in Biological and Non-Biological Samples. Med. 2019; 6:35.

Harborne JB. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. (2nd ed.). Bandung: ITB Press; 1987.

Hou C, Zhu X, Shi C, Peng Y, Huang D, Li Q, Miao Y. Iguratimod (T-614) attenuates severe acute pancreatitis by inhibiting the NLRP3 inflammasome and NF-κB pathway. Biomed Pharmacother. 2019; 119:1-7.

Leal S, Ricardo Jorge DO, Joana B, Maria SS, Isabel SS. Heavy Alcohol Consumption Effects on Blood Pressure and on Kidney Structure Persist After Long-Term Withdrawal. Kidney Blood Press Res. 2017; 42:664-675.

Tanguay P. Kratom in Thailand. Ser Legis Reform Drug Pol. 2011; 13:1-16.

Gao B and Bataller R. Alcoholic liver disease: pathogenesis and new therapeutic targets. Gastroenterol. 2011; 141:1572-1585.

Prameswari OM and Widjanarko SB. Uji efek ekstrak air daun pandan wangi terhadap penurunan kadar glukosa darah dan histopatologi tikus diabetes mellitus. Jurnal Pangan dan Agroindustr. 2014; 2:16-27.

Zima T, Fialová L, Mestek O, Janebová M, Crkovská J, Malbohan I, Stípek S, Mikulíková L, Popov P. Oxidative stress,

metabolism of ethanol and alcohol-related diseases. J Biomed Sci. 2001; 8:59-70.

Ekanto B, Syamsudin S, Hastuti TP. Pengaruh pemberian teh rosella (Hibiscus sabdariffa) terhadap kadar superoxide dismutase (antioksidan tubuh utama) pada tikus jantan remaja yang diberi alcohol. Jurnal Ilmiah Kesehatatan Keperawatan. 2012; 8:7-16.

Soto C, Recoba R, Barrón H, Alvarez C, Favari L. Silymarin increases antioxidant enzymes in alloxan-induced diabetes in rat pancreas. Comp Biochem Physiol C Toxicol Pharmacol. 2003; 136:205-212.

Mirzaei H, Masoudifar A, Sahebkar A, Zare N, Nahand JS, et al. MicroRNA: a novel target of curcumin in cancer therapy. J Cell Physiol. 2018; 233:3004-3015.

León F, Habib E, Adkins JE, Furr EB, McCurdy CR, Cutler SJ. Phytochemical characterization of the leaves of Mitragyna speciosa grown in U.S.A. Nat Prod Commun. 2009; 4:907-910.

Kruegel AC, Uprety R, Grinnell SG, Langreck C, Pekarskaya EA, Le Rouzic V, Ansonoff M, Gassaway MM, Pintar JE, Pasternak GW, Javitch JA, Majumdar S, Sames D. 7-Hydroxymitragynine Is an Active Metabolite of Mitragynine and a Key Mediator of Its Analgesic Effects. ACS Central Sci. 2019; 5:992-1001.

Hiranita T, Leon F, Felix JS, Restrepo LF, Reeves ME, Pennington AE, Obeng S, Avery BA, McCurdy CR, McMahon LR, Wilkerson JL. The effects of mitragynine and morphine on schedule-controlled responding and antinociception in rats. Psychopharmacol. 2019; 236:2725-2734.

Goh TB, Koh RY, Mordi MN, Mansor SM. Antioxidant value and antiproliferative efficacy of mitragynine and a silane reduced analogue. Asian Pac J Cancer Prev. 2014; 15:5659-5665.

Chang-Chien GC, Odonkor CA, Amorapanth P. Is Kratom the New 'Legal High' on the Block?: The Case of an Emerging Opioid Receptor Agonist with Substance Abuse Potential. Pain Physic. 2017; 20:E195-E198.