Expression of p16INK4a in the Brain of Animal Model of D-Galactose Induced Aging
doi.org/10.26538/tjnpr/v5i7.8
Keywords:
Animal model, D-galactose, Brain aging, p16INK4aAbstract
Oxidative stress and mitochondrial dysfunction play a major role in aging. Chronic administration of D-galactose has been reported to cause a decline in cognitive and motor skills similar to the symptoms of aging, therefore, it is considered a model of accelerated aging. The p16INK4a as aging biomarkers have been investigated. The study evaluated and compared natural aging and D-galactose induced aging model in the hippocampus. Mice were divided into 5 groups (n = 5), i.e., K0: young control (3 months of age), K1: natural aging (24 months of age), P1, P2, and P3: treatment group (3 months of age, D-galactose was administered subcutaneously at the doses of 100 mg/kgbw, 200 mg/kgbw, and 400 mg/kgbw, respectively) for 8 weeks. Hippocampus tissues were collected. The p16INK4a expression of hippocampus was evaluated by RT-PCR and immunohistochemistry test. The study showed weight gain in K1 (natural aging) and P3 (D-gal 400mg/kgbw) was significant (p = 0.010 and p = 0.019, respectively). The expression of p16INK4a in P1 (D-gal 100 mg/kgbw) was similar to naturally aging group and the highest expression compared to the others (p > 0.005). Immunoexpression of p16INK4 in P1 showed the highest increased expression (10.6 ± 1.95) compared to the other treatment groups (2.2 ± 1.3 and 1.2 ± 1.6, respectively). D-galactose inducted at dose 100 mg/kgbw for 8 weeks is a suitable animal model for aging brain.
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