Rosmarinic Acid Restored Testicular Function and Steroidogenic Hormone Capacity in Wistar Rats Exposed to Perfluorooctanoic Acid Toxicity

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Published: Sep 1, 2024
DOI: https://doi.org/10.26538/tjnpr/v8i8.27
Keywords:
Environmental toxicant, Sperm motility, Oxidative stress, Male infertility, Perfluorooctanoic acid, Rosmarinic acid

Main Article Content

Victoria A. Aluko
Department of Biochemistry, Faculty of Science and Technology, Bingham University, Karu, Nasarawa State, Nigeria
Shekins O. Okere
Department of Biochemistry, Faculty of Science and Technology, Bingham University, Karu, Nasarawa State, Nigeria
Janet O. Olugbodi
Department of Biochemistry, Faculty of Science and Technology, Bingham University, Karu, Nasarawa State, Nigeria
Moses D. Adams
Clinical Biochemistry, Phytopharmacology and Biochemical Toxicology Research Laboratory (CBPBT-RL), Department of Biochemistry, Baze University, Abuja 900108, Nigeria

Abstract

Perfluorooctanoic acid (PFOA) is an environmental toxicant which cause adverse health effects including male infertility and DNA damage in the germ line via altered oxidative stress activity. Rosmarinic acid (RA), one of the most important polyphenols, is a natural dietary nutraceutical with antioxidant property on adverse effects of toxicants. This study seeks to assess the restorative effect of RA on PFOA-induced toxicity in male Wistar rats. Twenty adult male rats (150-250g) were categorized into four groups (n =5). Animals in group 1 (control) took distilled water. Animals in categories 2 - 4 which were induced toxicity by PFOA (20 mg/kg body weight) were treated with distilled water, RA (40 mg/kg body weight) and Vitamin E (180 mg/kg body weight) respectively. Treatment was for 14 days. The epididymis was used for sperm analysis while blood sample was used for hormonal marker assay. Induction of PFOA substantively (p<0.05) lowered body weight, testes weight, sperm count, sperm motility, sperm morphology, testosterone, LH and FSH when compared with the distilled treated water treated control animals. Treatment of PFOA-induced toxic rats with RA significantly (p<0.05) restored epididymal sperm count, motility and morphology as well as body and testicular weight and those of serum hormone biomarkers in relation to Vitamin E. Results indicated that rosmarinic acid restored epididymal, testicular and hormonal PFOA-induced toxicity. This suggests that RA could be a potential therapeutic agent against PFOA- induced testicular damage.

Article Details

How to Cite
Aluko, V. A., Okere, S. O., Olugbodi, J. O., & Adams, M. D. (2024). Rosmarinic Acid Restored Testicular Function and Steroidogenic Hormone Capacity in Wistar Rats Exposed to Perfluorooctanoic Acid Toxicity. Tropical Journal of Natural Product Research (TJNPR), 8(8), 8106–8111. https://doi.org/10.26538/tjnpr/v8i8.27
Issue
Vol. 8 No. 8 (2024): Tropical Journal of Natural Product Research (TJNPR)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biography

Janet O. Olugbodi, Department of Biochemistry, Faculty of Science and Technology, Bingham University, Karu, Nasarawa State, Nigeria

Department of Medicine, Emory University School of Medicine, Atlanta, United States of America

References

Yuan Y, Ge S, Lv Z, Wu M, Kuang H, Kuang H, Yang B, Yang J, Wu L, Zou W, Zhang D. Attenuation of perfluorooctanoic acid-induced testicular oxidative stress and apoptosis by quercetin in mice. RSC Advan. 2017; 71: 45045 – 45052. DOI: 10.1039/C7RA09036F.

Bayasgalan G, Naranbat D, Radnaabazar J. Male infertility: Risk factors in Mongolian men. Asian J Androl. 2004; 6: 305-311.

Levine H, Jorgensen N, Martino-Andrade A, Mendiola J, Weksler-Derri D, Jolles M. Temporal trends in sperm count: a systematic review and meta-regression analysis of samples collected globally in the 20th and 21st centuries. Hum Reprod Update. 2023; 29(2): 157-76.

Huang B, Wang Z, Kong Y, Jin M, Ma L. Global, regional and national burden of male infertility in 204 countries and territories between 1990 and 2019: an analysis of global burden of disease study. BMC Publ. Health. 2023; 23: 2195. https://doi.org/10.1186/s12889-023-16793-3.

Sengupta P, Nwagha U, Dutta S, Krajewska-Kulak E, Izuka E. Evidence for decreasing sperm count in African population from 1965 to 2015. Afr Health Sci. 2017; 17(2): 418-27.

Akang EN, Opuwari CS, Enyioma-Alozie S, Moungala LW, Amatu TE., Wada I, Ogbeche RO, Ajayi OO, Aderonmu MM, Akinola LA, Ashiru OA. Henkel R. Male Infertility in Nigeria and South Africa: A Ten-Year Observational Study. Research Square. 2023; 1-11. https://doi.org/10.21203/rs.3.rs-2459901/v1.

Singh S, Das Roy L, Giri S. Curcumin protects metronidazole and X-ray induced cytotoxicity and oxidative stress in male germ cells in mice. Prague Med. Rep. 2013; 114: 92–102. doi:10.14712/23362936.2014.27.

Obiako, PC and Ayisire SO, Sayes CM. Impact of perfluorooctanoic acid (PFOA) and perfluorobutanoic acid (PFBA) on oxidative stress and metabolic biomarkers in human neuronal cells (SH-SY5Y). Environ. Int’l. 2024; 108864. J Pre-Proof. DOI:10.1016/j.envint.2024.108864.

Bulgakov VP, Inyushkina YV, Fedoreyev SA. Rosmarinic acid and its derivatives: biotechnology and applications. Critical Rev. Biotechnol. 2012; 32: 203–217.

Petersen M, Abdullah Y, Benner J, Eberle D, Gehlen K, Hucherig S, Janiak V, Kim KH, Sander M, Weitzel C, Wolters S. Evolution of rosmarinic acid biosynthesis. Phytochem. 2009; 70: 1663–1679.

Karthikkumar V, Sivagami G, Vinothkumar R, Rajkumar D, Nalini N. Modulatory efficacy of rosmarinic acid on premalignant lesions and antioxidant status in 1,2-dimethylhydrazine-induced colon carcinogenesis. Environ. Toxicol. Pharmacol. 2012; 34: 949–958.

Kuo CF, Su JD, Chiu CH, Peng CC, Chang CH and Sung TY. Anti-inflammatory effects of supercritical carbon dioxide extract and its isolated carnosic acid from Rosmarinus officinalis leaves. J. Agric. Food Chem. 2011; 59: 3674–3685.

Asl JF, Goudarzi M, Mansouri E, Shoghi H. Rosmarinic Acid Protects the Testes of Rats against Cell Phone and Ultra-high Frequency Waves Induced Toxicity. Iran J Med Sci. 2024; 49(4): 237–246. DOI: 10.30476/ijms.2023.97695.2952.

Asır F, Gokalp-Ozkorkmaz E, Deveci E, Sen G, Ayaz H, Kaplan S. Effects of rosmarinic acid on a rat model of testicular torsion/detorsion: an immunohistochemical study. Dicle University, Diyarbakır, Turkey. Int’l Health Sci Conf (IHSC). 2019; 608-616.

Oguz A, Boyuk A, Ekinc A, Aday U. Investigation of antioxidant effects of rosmarinic acid on liver, lung and kidney in rats: A biochemical and histopathological study. Folia Morphol. 2015; 79(2): 32-39. DOI: 10.5603/FM.a2019.0087.

Sahin F, Asır F, Ozkorkmaz E, Basaran S, Kaplan O, Ermis I, Deveci, E. Investigation of the Effect of Rosmarinic Acid on Cyclophosphamide-Induced Gonadal Toxicity. Advan. Sex Med. 2022; 12: 1-8. DOI: 10.4236/asm.2022.121001.

Uyeturk U, Uyeturk U, Firat T, Cetinkaya A, Tekce BK, Serkan CS. Protective effects of rosmarinic acid on doxorubicin-induced testicular damage. Chemother. 2014; 60(1):7- 12. DOI: 10.1159/000365727.

Arash A, Imani SAM, Golzar FS. Effects of rosmarinic acid on male sex hormones (testosterone-FSH-LH) and testis tissue apoptosis after exposure to electromagnetic field (EMF) in rats. African J. Pharm. Pharmacol. 2012; 6(4): 248-252. DOI: 10.5897/AJPP11.701 ISSN 1996-0816.

Meng-Ge LV, Wen-Qiong C, Shi-Gi W, Hou-Yang C, Yi-Min C, Tao L. Rosmarinic acid compromises human sperm functions by an intracellular Ca2+ concentration-related mechanism. Reprod. Toxicol. 2018; 81: 58-63.

Al-Alami ZM, Shraideh ZA, Taha MO. Rosmarinic acid reverses the effect of metronidazole-induced infertility in male albino rats. Reprod. Fertil. Dev. 2017; 29(10):1910-1920. DOI: 10.1071/RD16174.

Farzadi L, Khaki A, Ghasemzadeh A, Ouladsahebmadarek E, Ghadamkheir E, Shadfar S, Khaki AA. Effect of rosmarinic acid on sexual behavior in diabetic male rats. African J. Pharm. Pharmacol. 2022; 5(16): 1906-1910. https://doi.org/10.5897/AJPP11.463.

Deger U. and Cavus Y. Investigation of the role of rosmarinic acid treatment in regulating inflammation, cell damage, and angiogenesis in rat ovarian torsion and detorsion models. Acta Cir. Bras. 2020; 35 (3): 52-9. https://doi.org/10.1590/s0102-865020200030000004.

Farsi A, Khaki A, Fathiazad F, Afshari F, Hajhossini L, Kahki AF. Improvement Effect of Rosmarinic Acid on Serum

Yuan Y, Ge S, Lv Z, Wu M, Kuang H, Kuang H, Yang B, Yang J, Wu L, Zou W, Zhang D. Attenuation of perfluorooctanoic acid-induced testicular oxidative stress and apoptosis by quercetin in mice. RSC Advances. 2017; 71: 45045–45052. DOI: 10.1039/C7RA09036F.

Nwaechefu OO, Arojojoye OA, Oyedeji TA, Ojo OO, Balogun OA, Kassim S. Influence of Lophira lanceolata leaf Extract on reproductive indices and haematological parameters in Male Wistar rats. Trop J Nat Prod Res. 2021; 5(4):744-748. http://dx.doi.org/10.26538/tjnpr/v5i4.24.

Oyeyemi WA, Shittu ST, Kolawole TA, Ubaneche P, Akinole AO. Protective Effect of Vitamin E on Nicotine Induced Reproductive Toxicity in Male Rats. Nigerian J Basic Appl. Sci 2015; 23(1): 7-13 DOI: http://dx.doi.org/10.4314/njbas.v23i1.2.

Fauser BCJM, Van-Heusden AM. Manipulation of human ovarian function: physiological concepts and clinical consequences. Endocr Rev. 1997; 18:71–106.

Chen A, Bookstein JJ, Meldrum DR. Diagnosis of a testosterone-secreting adrenal adenoma by selective venous catheterization. Fertil. Steril. 1991; 55: 1202-1203.

Clinical Guide to Laboratory Tests. Ed. N.W. Tietz, 3rd Ed., W.B Saunders Company, Philadelphia, PA 19106, 1995.

Alamo A, Vignera SL, Mogioi LM, Crafa A, Barbagallo F, Cannarella R, Aversa A, Calogero AE, Condorelli RA. In vitro effects of perfluorooctanoic acid on human sperm function: What are the clinical consequences? J Clin Med. 2024; 13(8): 2201. DOI: 10:3390/jcm13082201.

Fadel O, El-Kirat K, Morandat S. The natural antioxidant rosmarinic acid spontaneously penetrates membranes to inhibit lipid peroxidation in situ. Biochim Biophys Acta. 2011; 1808 (12): 2973–2980.

Santi D, Lotti F, Sparano C, Rastrelli G, Isidori AM, Pivonello R, Barbonetti A, Salonia A, Minhas S, Krausz C, Vignozzi L, Maggi M, Corona G. Does an increase in adipose tissue 'weight' affect male fertility? A systematic review and meta-analysis based on semen analysis performed using the WHO 2010 criteria. 2024; 12(1):123-136.DOI: 10.1111/andr.13460.

Bellurkar A, Patwardhan S, Patil B, Kanbur A, Jain H, Rishikesh VR. Role of Testicular Size as a Parameter for Predicting Infertility in Indian Males. J Hum Reprod Sci. 2020; 13(2): 114–116. DOI: 10.4103/jhrs.JHRS_7_20.

Zafar MI. and Chen X. Effects of Calorie Restriction on Preserving Male Fertility Particularly in a State of Obesity. Curr Obes Rep. 2024; Online ahead of print. DOI: 10.1007/s13679-024-00557-0.

Yakubu MT and Omar SA. Impact of groundwater samples and leachates from Gbagede dumpsite, Amoyo, Kwara State, Nigeria, on testes and prostate of male Wistar rats: A biochemical and histological study. 2020; 52 (11):e13801. https://doi.org/10.1111/and.13801.

Norris AC, Yazlovitskaya EM, Yang TS, Mansueto A, Stafford JM, Graham TM. (2024). ATP10A deficiency results in male-specific infertility in mice. Front. Cell Dev. Biol.2024; 12-2024. DOI: 10.3389/fcell.2024.1310593.

Fink J, Ide H, Horie S. Management of Male Fertility in Hypogonadal Patients on Testosterone Replacement Therapy. Medicina. 2024; 60(2): 275. https://doi.org/10.3390/medicina60020275.

Pan F, Fu W, Zhang B, Han M, Xie H, Yi Q, Qian W, Cui J, Cao M, Li Y, Jia Y, Fang F, Ling Y, Li Y, Liu Y. Effects of Vaccination against Recombinant FSH or LH Receptor Subunits on Gonadal Development and Functioning Male Rats. Vet. Sci. 2024; 11(4):176. DOI: 10.3390/vetsci11040176. DOI: 10.3390/vetsci11040176.

Santi D, Crepieux P, Reiter E, Spaggiari G, Brigante G, Casarini L, Rochira, V, Simoni M. Follicle-Stimulating Hormone (FSH) Action on Spermatogenesis: A Focus on Physiological and Therapeutic Roles. J Clin. Med. 2020; 9(4): 1014. DOI: 10.3390/jcm9041014.