Hydrogen-Rich Water Ameliorates Pancreatic Cell Necrosis and Regulates Cytokine Levels in Streptozotocin-Induced Diabetic Rats
doi.org/10.26538/tjnpr/v5i8.6
Keywords:
Diabetes mellitus, Hydrogen-rich water, IL-1β, IL-10, NecrosisAbstract
Water plays an important role in the metabolic processes. An unhealthy lifestyle leads to an increase in free radicals, which causes type 2 diabetes mellitus (T2DM). Hydrogen-rich water (HW) is water with dissolved hydrogen gas and has been implicated to have antioxidant activity. This study was aimed at analyzing the effect of HW on pancreatic cell necrosis and cytokine level in streptozotocin (STZ)-induced diabetic rats. Male Wistar rats were fed a high-fat diet (HFD) for four weeks before being injected intraperitoneally with low dose STZ (35 mg/kg bw) to obtain DM rats. Blood glucose values of >200 mL/dL were considered diabetic rats. The experimental rats were randomly divided into five groups: healthy (normal rats without STZ injection), DM, DM + metformin (45 mg/kg bw), DM + HW, and DM + HW + metformin. The treatments were administered orally for fourteen days. After that, the rats were dissected using Ket-A-Xyl; serum and liver samples were obtained to determine the concentrations of IL-10 and IL-1β. The pancreas was collected and examined for cell damage, while histology of the Islet of Langerhans was analyzed descriptively. The results showed that HW reduced blood glucose levels, body weight, and IL-1β concentration, increased IL-10 concentration, and improvednecrotic cells of the pancreas in comparison to DM rats. HW could be one of the therapies for T2DM patients by lowering glucose levels, regulating cytokine levels, and repairing β-cells in the pancreas.
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