Characteristics and In Vitro Antibacterial Activity of Lactobacillus acidophilus FNCC-0051 Probiotic Microspheres against Propionibacterium acnes ATCC 11827
doi.org/10.26538/tjnpr/v5i8.4
Keywords:
Probiotics, Microspheres, Antibacterial activity, pHAbstract
Lactobacillus acidophilus FNCC-0051 is a probiotic which contains bacteriocin metabolites, lactic acid, and organic acid which are effective as antibacterial for acne treatment. This study aims to encapsulate probiotic, characterise and determine the antibacterial activity of L. acidophilus FNCC-0051 at pH 4.5 and 6.0. The probiotic microspheres were made using ionic gelation aerosolization technique with 2% sodium alginate and 1.0 M CaCl2. The characterization included surface morphology, size, moisture content, swelling index, and viability. Antibacterial activity was carried out by diffusion method using Propionibacterium acnes ATCC 11827. Activity was determined by inhibition zone diameter every 1 hour for 6 hours. Results showed that the microsphere was spherical with smooth surface with a size of 1.7841 ± 0.0253 µm. Moisture content was 3.38 ± 0.18% and the swelling index at pH 4.5 and pH 6.0 were 384.29% and 507.37%, respectively within 6 hours. The results of inhibition zone diameter measurement against Propionibacterium acnes at pH 4.5 for 6 hours were 10.11 ± 1.07; 12.16 ± 1.25; 15.73 ± 2.36; 18.81 ± 3.20; 22.99 ± 2.38; and 30.74 ± 5.24 mm. while the inhibition zone diameter at pH 6.0 were 20.98 ± 2.41; 25.58 ± 2.40; 31.04 ± 3.57; 36.09 ± 2.62; 40.47 ± 2.12; and 48.38 ± 4.73 mm. Swelling capacity at pH 6.0 was higher than at pH 4.5. Therefore, the released probiotics were higher at pH 6 and caused a higher antibacterial activity at pH 6.0 than at pH 4.5.
References
Ozogul F, Yasgan H, Ozogul Y. Lactic Acid Bacteria: Lactobacillus spp: Lactobacillus acidophilus. Ref Module
Food Sci. 2016; 2016:1-10.
Shokryazdan P, Sieo CC, Kalavathy R, Liang JB, Alitheen NB, Faseleh JM, Ho YW. Probiotic potential of
Lactobacillus strains with antimicrobial activity against some human pathogenic strains. Biomed Res Int. 2014; 2014:927268.
Achermann Y, Goldstein EJ, Coenye T, Shirtliff ME. Propionibacterium acnes: from commensal to opportunistic
biofilm-associated implant pathogen. Clin Microbiol Rev. 2014; 27(3):419-440.
Bowe WP, Patel NB, Logan AC. Acne vulgaris, probiotics and the gut-brain-skin axis: from anecdote to translational medicine. Benef Microbes. 2014; 5(2):185-199.
Kim SJ, Cho SY, Kim SH, Song OJ, Shin IS, Cha DS, Park HJ. Effect of microencapsulation on viability and other
characteristics in Lactobacillus acidophilus ATCC 43121. LWT-Food Sci Technol. 2008; 41(3):493-500.
Lengyel M, Kállai-Szabó N, Antal V, Laki AJ, Antal I. Microparticles, microspheres, and microcapsules for
advanced drug delivery. Sci Pharm 2019; 87(3):1-31.
Gupta AK and Dey BK. Microencapsulation for Controlled Drug Delivery: A Comprehensive Review. Sunsari Tech
Coll J. 2012; 1(1):48-54.
Chen X, Peng L, Gao J. Novel Topical Drug Delivery System and their Potential Use in Scars Treatment. Asian J
Pharm Sci. 2012; 7(3):155-167.
Hariyadi DM, Purwanti T, Wardani D. Stability of FreezeDried Ovalbumin-Alginate Microspheres with Different Lyoprotectants. Res J Pharm Technol. 2016; 9(1):20-26.
Mortazavian M, Ehsani R, Azizi A, Razavi M, Mousavi S, Reinheimer A. Viability of Calcium-Alginate-Microencapsulated Probiotic Bacteria in Iranian Yogurt Drink (Doogh) during Refrigerated Storage and Under
Simulated Gastrointestinal Conditions. Aust J Dairy Technol. 2008; 63(1):24-28.
Xinhuai Z, Zhang Y, Li D. Elimination of Acidic or Oxidative Stress for Four Probiotics with Some Chemicals
In vitro. Afr J Microbiol Res. 2009; 3(7): 353-357.
Bajpai SK and Narendra K. Swelling and Drug Release Behavior of Calcium Alginate/Poly (Sodium Acrylate)
Hydrogel Beads. Des Monomers Polym. 2016; 19(1):89-98
Reddy KV, Ramana, Nagabhushanam MV. Process and Parameters Affecting Drug Release Performance of
Prepared Cross-Linked Alginate Hydrogel Beads for Ezetimibe. Int J Pharm Pharm Sci. 2017; 9(2):254-262.
Lin W, Hwang C, Chen L, Tsen H. Viable counts, characteristic evaluation for commercial lactic acid bacteria
product. Food Microbiol. 2006; 23:74-81.
Himeoka Y and Kaneko K. Theory for Transitions between Exponential and Stationary Phases: Universal Laws for Lag Time. Phys Rev X. 2017; 7(2):1-25.
Purwanti T, Soeratri W, Zainudin M. Characterization of Nisin Microspheres with Combination Matrix Sodium
alginate – Gelatin. Int J Pharm Res Health Sci. 2018; 6(6): 2838–2843.
Saini S, Kumar S, Choudhary M, Budhwar V. Microspheres as Controlled Drug Delivery System: an Updated Review. Int J Pharm Sci Res. 2018; 9(5):1760-1768.
Solanki HK and Shah DA. Formulation optimization and evaluation of probiotic Lactobacillus sporogenes-loaded sodium alginate with carboxymethyl cellulose mucoadhesive beads using design expert software. J Food
Proc. 2016; 2016: Article ID 6041671, 14 pages.
Tingting Z, Zhu W, Wang Q, He L, Wu W, Liu J, Li Y, SunD. Antibiotic Susceptibility of Propionibcterium acnes
Isolated from Patients with Acne In a Public Hospital in Southwest China: Prospective cross-sectional study. Br
Med J. 2019; 9: 1-7.
Bhowmik D, Gopinath H, Kumar BP, Duraivel S, Kumar KPS. Recent Advances In Novel Topical Drug Delivery
System. J Pharm Innov. 2012; 1(9):12-31.
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
