Fagonia longispina Batt. extracts: Antioxidant, Antibacterial, and Antiproliferative effects on Cancer Cells

Article Sidebar

pdf epub
Published: Jun 29, 2024
DOI: https://doi.org/10.26538/tjnpr/v8i6.6
Keywords:
antiproliferative activity, antibacterial activity, antioxidant activity, lipid peroxidation, phenolic compounds, Fagonia longispina

Main Article Content

Radja Djebbari
Department of Organism Biology, Batna University, 2–53, Road to Constantine. Fésdis, Batna 05078, Algeria.
Somia Lassed
Department of Microbiology and Biochemistry, Batna University, 2–53, Road to Constantine. Fésdis, Batna, 05078, Algeria.
Widad Ourzeddine
Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.
Sana Aouachria
Department of Microbiology and Biochemistry, Batna University, 2–53, Road to Constantine. Fésdis, Batna, 05078, Algeria.
Muhammed Altun
Plant Research Laboratory, Department of Chemistry, Faculty of Science, Çankırı Karatekin University, Uluyazı Campus, 18100 Çankırı, Turkey
Ibrahim Demirtas
Plant Research Laboratory, Department of Chemistry, Faculty of Science, Çankırı Karatekin University, Uluyazı Campus, 18100 Çankırı, Turkey.
Amina M. Lehneche
Department of Natural Science, Training School of Teachers "Assia Djebar", Constantine University Town Ali Mendjli, Constantine, Algeria.
Scheherazed Dakhmouche-Djekrif
Department of Natural Science, Training School of Teachers "Assia Djebar", Constantine University Town Ali Mendjli, Constantine, Algeria.
Mohammed Kebieche
LMAGECA and BMBP research laboratories, Batna University 2–53, Road to Constantine. Fésdis, Batna 05078, Algeria
Fadila Benayache
Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.
Samir Benayache
Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.
Djamila Zama
Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.

Abstract

Fagonia longispina (Zygophyllaceae family) has long been employed in traditional practices for combating cancer, with scientific research yielding insights into its biological impact. This study aimed to validate the potential of this plant by evaluating the antioxidant, antiproliferative, and antibacterial properties of the n-butanol, ethyl acetate, and chloroform extracts of F. longispina (BuE, EAE, and ChE, respectively). The most significant total phenolics (TPC) were recorded in EAE (377 ± 24.33 µg GAE/mg extract), whereas BuE showed the most important quantity of flavonoids (TFC) (87.14 ± 1.56 µg QE/mg extract). Outcomes indicated that EAE displayed the highest DPPH (2, 2-diphenyl-1-picrylhydrazyl) scavenging effect (IC50 = 37.82 ± 7.83 μg/mL) compared to BuE (IC50 = 59.49 ± 6.16 μg/mL, p = 0.0197) and ChE (IC50 =111.89 ± 6.10 μg/mL, p = 0.0002). Despite the increased activity of EAE, it remained lower than ascorbic acid (IC50 = 5.00 ± 0.1 µg/mL, p = 0.02). Moreover, EAE was the strongest at scavenging free radicals from FeSO4 (IC50 = 126.66 ± 5.76 µg/mL). All extracts except BuE showed antiproliferative efficacy against HeLa and HT-29 cells. Various bacterial strains underwent testing to demonstrate their antibacterial effectiveness, which was assessed by measuring inhibition zones. The results showed enjoyable antimicrobial activity against gram-positive bacteria. In conclusion, this study proves the application of F. longispina in traditional medicine. Furthermore, the results from the antioxidant tests confirmed that F. longispina had an antioxidant effect and may be used as a new natural antioxidant in therapy.  

Downloads

Download data is not yet available.

Article Details

How to Cite
Djebbari, R., Lassed, S., Ourzeddine, W., Aouachria, S., Altun, M., Demirtas, I., Lehneche, A. M., Dakhmouche-Djekrif, S., Kebieche, M., Benayache, F., Benayache, S., & Zama, D. (2024). Fagonia longispina Batt. extracts: Antioxidant, Antibacterial, and Antiproliferative effects on Cancer Cells. Tropical Journal of Natural Product Research (TJNPR), 8(6), 7374-7381. https://doi.org/10.26538/tjnpr/v8i6.6
Issue
Vol. 8 No. 6 (2024): Tropical Journal of Natural Product Research (TJNPR)
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biographies

Radja Djebbari, Department of Organism Biology, Batna University, 2–53, Road to Constantine. Fésdis, Batna 05078, Algeria.

Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.

Somia Lassed, Department of Microbiology and Biochemistry, Batna University, 2–53, Road to Constantine. Fésdis, Batna, 05078, Algeria.

Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.

Djamila Zama, Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.

 

 

How to Cite

Djebbari, R., Lassed, S., Ourzeddine, W., Aouachria, S., Altun, M., Demirtas, I., Lehneche, A. M., Dakhmouche-Djekrif, S., Kebieche, M., Benayache, F., Benayache, S., & Zama, D. (2024). Fagonia longispina Batt. extracts: Antioxidant, Antibacterial, and Antiproliferative effects on Cancer Cells. Tropical Journal of Natural Product Research (TJNPR), 8(6), 7374-7381. https://doi.org/10.26538/tjnpr/v8i6.6

References

Odoemelam EI, Ugorji CO, Ezema BE, Agbo MO, Nnadi CO, Orjiocha SI, Okonkwo VI, Nwafor FI, Ugwu GN, Chukwuma MO. Estimation of Total Phenolics, Total Flavonoids Content and In Vitro Antioxidant Activities of Extract and Fractions of Asplenium platyneuron (Carl Linnaeus). Trop. J. Nat. Prod. Res. 2024; 8(3): 6723–6730. https://doi.org/10.26538/tjnpr/v8i3.36.

Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, Gargiulo G, Testa G, Cacciatore F, Bonaduce D, Abete P. Oxidative stress, aging, and diseases. Clin Interv Aging. 2018; 13:757-772. https://dx.doi.org/10.2147/CIA.S158513.

Gulcin I. Antioxidants and antioxidant methods: an updated overview. Arch Toxicol. 2020; 94(3): 651–715. https://dx.doi.org/10.1007/s00204-020-02689-3.

Mahraz MA, Idrissi MA, Mzali A, Mrayej HE, Lfatouhi A, Salimx R, Loukili EH, Jghaoui M, Rais Z, Taleb M. Ethnobotanical Survey of Medicinal and Aromatic Plants used in the Treatment of Skin Burns in the Province of Sefrou of Morocco. Trop. J. Nat. Prod. Res. 2024; 8(3): 6487–6497. https://doi.org/10.26538/tjnpr/v8i3.3.

Wangkheirakpam S. Traditional and Folk Medicine as a Target for Drug Discovery. In Natural Product and Drug Discovery. Elsevier. 2018; 29-56. https://dx.doi.org/10.1016/B978-0-08-102081-4.00002-2.

Chen SL, Yu H, Luo HM, Wu Q, Li CF, Steinmetz A. Conservation and sustainable use of medicinal plants: problems, progress, and prospects. Chin med. 2016; 11(37): 1-10. https://dx.doi.org/10.1186/s13020-016-0108-7.

Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines. 2018; 5(3): 93. https://dx.doi.org/10.3390/medicines5030093.

Hamidi N, Lazouni HA, Moussaoui A, Ziane L, Djellouli M, Belabbesse A. Ethnopharmacology, Antibacterial and Antioxidant Activities, Phytochemical Screening of Bioactive Extracts From the Aerial Parts of Fagonia Longispina. Asian J Nat Appl Sci. 2014; 3(3): 53-63.

Ourzeddine W, D'Ambola M, Malafronte N, León F, Brouard I, Benayache F, Benayache S. A New Δ-2-Carene-β-D-glucopyranoside from Fagonia longispina. Nat Prod Commun. 2017; 12(9): 1381–1383.

Quezel P., Santa S. New flora of Algeria and the desert regions Meridional. French National Center for Scientific Research, Paris; 1963.

Djellouli M, Berghioua A. Chemical composition, antioxidant and antimicrobial activity of Fagonia longispina (Zygophyllaceae) of Algerian. Biodiversitas. 2021; 22(6): 3448-3453.

Hamidi N, Ziane L, Djellouli M, Lazouni H. Chemical Characterization By Gc-Ms From The Aerial Parts Of Fagonia Longispina (Zygophyllaceae). Asian J Pharm Clin Res. 2016; 9(1): 175-176.

Laraba M, Tachour SH, Belbache H, Boubekri N, Djebbari R, Benayache F, Benayache S, Zama D. Hepatoprotective potential of the n-butanol extract of Moricandia arvensis from Algeria against doxorubicin induced toxicity in Wistar albino rats. Adv. Tradit. Med. 2022; 22(4): 853-864. https://doi.org/10.1007/s13596-022-00642-6.

Mecheri A, Amrani A, Benabderrahmane W, Bensouici C, Boubekri N, Benaissa O, Zama D, Benayache F, Benayache S. In vitro pharmacological secreening of antioxidant, photoprotective, cholineterase, and α-glucosidase inhibitory activities of algerian Crataegus oxyacantha Fruits and leaves extracts. Pharm. Chem. J. 2021; 54(11): 1150-1156.

Bicha S, Amrani A, Benaissa O, León F, Zama D, Brouard I, Benayache S, Bentamen A, Benayache F. A flavonoid with high antioxidant effect from Centaurea acaulis L. Pharm Lett. 2013; 5(6): 24-30.

Ijoma KI, Egbulefu AVI, Odinma SC. The organic extracts from the leaves of Ficus thonningii Blume, Jatropha tanjorensis J. L Ellis and Saroja and Justicia carnea Lindley as potential nutraceutical antioxidants and functional foods. Trends Phytochem. Res. 2023; 7(1): 76-85.

Cao Y, Ikeda I. Antioxidant activity and antitumor activity (in vitro) of xyloglucan selinious ester and surfated xyloglucan. Int. J. Biol. Macromol. 2009; 45(3): 231-235. https://dx.doi.org/10.1016/j.ijbiomac.2009.05.007.

Koldaş S, Demirtas I, Ozen T, Demirci MA, Behçet L. Phytochemical screening, anticancer and antioxidant activities of Origanum vulgare L. ssp. viride (Boiss.) Hayek, a plant of traditional usage. J. Sci. Food. Agric. 2015; 95(4): 786-798.

Shakeri A, Hazeri N, Vlizadeh J, Ghasemi A, Tavallaei FZ. Phytochemical screening, Antimicrobial and antioxidant activities of Anabasis aphylla L. extracts. Kragujevac J Sci. 2012; 34(34): 71-78.

Zaidan MR, Noor Rain A, Badrul AR, Adlin A, Norazah A, Zakiah I. In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. Trop Biomed. 2005; 22(2): 165–170.

Twaij BM, Hasan MN. Bioactive Secondary Metabolites from Plant Sources: Types, Synthesis, and Their Therapeutic Uses. Int Plant Biol. 2022; 13(1): 4-14. https://dx.doi.org/10.3390/ijpb13010003.

Abrahim NN, Kanthimathi MS, Abdul-Aziz A. Piper betle shows antioxidant activities, inhibits MCF-7 cell proliferation and increases activities of catalase and superoxide dismutase. BMC Complement Altern Med. 2012; 12: 1-12.

Jariene E, Lasinskas M, Danilcenko H, Vaitkeviciene N, Slepetiene A, Najman K, Hallmann E. Polyphenols, Antioxidant Activity and Volatile Compounds in Fermented Leaves of Medicinal Plant Rosebay Willowherb (Chamerion angustifolium (L.) Holub). Plants. 2020; 9(12): 1683. https://dx.doi.org/10.3390/plants9121683.

Taïlé J, Arcambal A, Clerc P, Gauvin-Bialecki A, Gonthier MP. Medicinal Plant Polyphenols Attenuate Oxidative Stress and Improve Inflammatory and Vasoactive Markers in Cerebral Endothelial Cells during Hyperglycemic Condition. Antioxidants. 2020; 9(7): 573. https://dx.doi.org/10.3390/antiox9070573.

Djebbari R, Chemam Y, Amrani A, Lassed S, Boubekri N, Zama D, Benayache F, Benayache, S. In vitro antioxidant activities of n-butanol extract of Helianthemum confertum. Int J Phytomed. 2015; 7: 119–122.

Iqbal P, Ahmed D, Asghar MN. A comparative in vitro antioxidant potential profile of extracts from different parts of Fagonia cretica. Asian Pac J Trop Med. 2014; 7: 473-480. https://dx.doi.org/10.1016/S1995-7645(14)60277-7.

Rashid U, Muhammad RK, Moniba S. Hepatoprotective potential of Fagonia olivieri DC. against acetaminophen induced toxicity in rat. BMC Complement Altern Med. 2016; 16(1): 1-18. https://dx.doi.org/10.1186/s12906-016-1445-x.

Zahnit W, Smara O, Bechki L, Dekmouche M, Bensouici C. In-vitro Assessment of Anti-Cholinesterase, Anti-Lipase, Antioxidant Activities and Photoprotective Effect of Algerian Fagonia bruguieri DC Extracts. Pharm Chem J. 2023; 57(1): 89–100. https://doi.org/10.1007/s11094-023-02855-8.

Mouffouk C, Mouffouk S, Oulmi K, Mouffouk S, Haba H. In vitro photoprotective, hemostatic, anti-inflammatory and antioxidant activities of the species Linariascariosa Desf. S Afr J Bot. 2020; 130:383-388. https://dx.doi.org/10.1016/j.sajb.2020.01.003.

Amir M, Khan A, Mujeeb M, Ahmad A, Usmani S, Akhtar M. Phytochemical Analysis and in vitro Antioxidant Activity of Zingiber officinale. Free Radic Antioxid. 2011; 1(4): 75-81. https://dx.doi.org/10.5530/ax.2011.4.12.

Gul MZ, Ahmad F, Kondapi AK, Qureshi IA, Ghazi IA. Antioxidant and antiproliferative activities of Abrus precatorius leaf extracts-an in vitro study. BMC Complement Altern Med. 2013; 13(1): 1-12. https://dx.doi.org/10.1186/1472-6882-13-53.

Lahneche AM, Boucheham R, Ozen T, Altun M, Boubekri N, Demirtas I, Bicha S, Bentamen A, Benayache F, Benayache S, Zama D. In vitro antioxidant, DNA-damaged protection and antiproliferative activities of ethyl acetate and n-butanol extracts of Centaurea sphaerocephala L. Anais Acad Brasil Ci. 2019: 91. http://dx.doi.org/10.1590/0001-3765201920180462.

Bouayed J, Bohn T. Exogenous antioxidants--Double-edged swords in cellular redox state: Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxid Med Cell Longev. 2010; 3(4): 228-237.

Djebbari R, Chemam Y, Boubekri N, Lakroun Z, Kebieche M, Amrani A, Benayache F, Benayache S, Zama D. Chemoprotective Potential of Helianthemum confertum Against the Loss of Molecular and Functional Integrity of the Liver Cell in Doxorubicin-Treated Rats. Int J Pharmacol Pharm Res. 2017; 9(7): 903-910.

Chemam Y, Benayache S, Marchioni E, Zhao M, Mosset P, Benayache F. On-Line Screening, Isolation and Identification of Antioxidant Compounds of Helianthemum ruficomum. Molecules. 2017; 22(2): 239. https://dx.doi.org/10.3390/molecules22020239.

Miranda CL, Kumbi Y, Wu W, Lee HS, Reed RL, Stevens J. Phytochemical Characterization and Bioactivity Toward Breast Cancer Cells of Unhydrolyzed and Acid-Hydrolyzed Extracts of Fagonia indica. Nat Prod Commun. 2022; 17(7): 1934578X221109426. https://dx.doi.org/10.1177/1934578X221109426.

Ismail H, Mostafa ME, El-Demerdash A, Hanna DM, Abdel-Mogib M. A new triterpene saponin from Fagonia schimperi. J Appl Pharm Sci. 2020; 10(12): 068-074. https://dx.doi.org:10.7324/JAPS.2020.101209.

Shad AA, Din ZU, Bakht J, Jan S. Antimicrobial, antioxidant potential and phytochemical screening of Fagonia olivieri. Pak J Pharm Sci. 2017; 30(3): 697-703.

Sulieman AME, Alanaizy E, Alanaizy NA, Abdallah EM, Idriss H, Salih ZA, Ibrahim NA, Ali NA, Ibrahim SE, Abd El Hakeem BS. "Unveiling Chemical, Antioxidant and 39.Antibacterial Properties of Fagonia indica Grown in the Hail Mountains, Saudi Arabia". Plants. 2023; 12(6): 1354. https://dx.doi.org/10.3390/plants12061354.

Iqbal M, Ullah N, Ibrahim M, Ahmed U, Jabbar A. In-Vitro Antibacterial Study of Fagonia Cretica. Int J Recent Res Asp. 2015; 4: 6-9.

Rahman L, Shinwari ZK, Iqrar I, Rahman L, Tanveer F. An assessment on the role of endophytic microbes in the therapeutic potential of Fagonia indica. Ann Clin Microbio Antimicrob. 2017; 16: 1-12. https://dx.doi.org/10.1186/s12941-017-0228-7.

Rehman KU, Hamayun M, Khan SK, Ahmad N, Wlai S. Efficiency of Virgin's Mantle (Fagonia cretica L.) as an Antibacterial and Antifungal Agent. Adv Life Sci. 2021; 8(3): 233-237.