Stability Study and Antifungal Activity of Chitosan Films from Shrimp Shells against Colletotrichum gloeosporioides

Authors

  • Thuan D.H. Nguyen Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City 70000, Vietnam.
  • Quyen T. Pham Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City 70000, Vietnam.
  • Khanh K. Nguyen Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City 70000, Vietnam.
  • Tuan N. Nguyen Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City 70000, Vietnam

DOI:

https://doi.org/10.26538/tjnpr/v8i6.2

Keywords:

Stability, Colletotrichum gloeosporioides, Chitosan film, Antifungal

Abstract

Chitosan, a modified product of chitin has found application in various fields such as in foods, cosmetics, agricultural products, and pharmaceuticals. This study aim to evaluate the stability and the antifungal activity of chitosan films produced from shrimp shells. Chitosan films with various degrees of deacetylation (70%, 80%, and 90%) were produced from shrimp shells by solution casting and solvent evaporation method. The stability of the chitosan films was evaluated under a controlled environmental conditions over a 35 days period. The key stability parameters monitored include; tensile strenght, elongation at break, elastic modulus, and water vapour permeability. The antifungal activity of the films was investigated against Colletotrichum gloeosporioides using the disc diffusion method. After 35 days, the mechanical properties (tensile strenght, elongation at break, and elastic modulus), and water vapour permeability of the chitosan films remained largely unchanged, indicating sustained stability over time. The chitosan films at the three deacetylation levels (70%, 80%, and 90%) exhibited antifungal activity against Colletotrichum gloeosporioides, with chitosan film with 90% degree of deacetylation showing the highest antifungal activity. From the results of the study, chitosan films at three degrees of deacetylation (70%, 80%, and 90%) have good stability under the storage conditions tested. They are highly active against Colletotrichum gloeosporioides, thus could be used as protective films for food preservation.

Author Biography

Khanh K. Nguyen, Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City 70000, Vietnam.

Faculty of Food Technology, Binh Duong University, Binh Duong Province 75000, Vietnam.

References

Domard A and Domard M. Chitosan: structure-properties relationship and biomedical applications. Polym Biomater. 2001; 2:187-212.

Andonegi M, Heras KL, Santos-Vizcaíno E, Igartua M, Hernandez RM, Caba K, Guerrero P. Structure-properties relationship of chitosan/collagen films with potential for biomedical applications. Carbohydr. Polym. 2020; 237(1):116159.

Rinaudo M, Pavlov G, Desbrieres J. Influence of acetic acid concentration on the solubilization of chitosan. Polymer. 1999; 40(25):7029-7032.

Kumar MNR. A review of chitin and chitosan applications. React Funct Polym. 2000; 46(1):1-27.

Parhi R. Drug delivery applications of chitin and chitosan: a review. Environ Chem Lett. 2020; 18:577-594.

Coma V, Deschamps A, Martial, Gros A. Bioactive packaging materials from edible chitosan polymer - antimicrobial activity assessment on dairy‐related contaminants. J Food Sci. 2003; 68(9):2788-2792.

Yan D, Li Y, Liu Y, Li N, Zhang X, Yan C. Antimicrobial Properties of Chitosan and Chitosan Derivatives in the Treatment of Enteric Infections. Molecules. 2021; 26(23):7136.

Sudarshan N, Hoover D, Knorr D. Antibacterial action of chitosan. Food Biotechnol. 1992; 6(3):257-272.

Ke CL, Deng FS, Chuang CY, Lin CH. Antimicrobial Actions and Applications of Chitosan. Polymers. 2021; 13(6):904.

Phuong, NNM, Ha LTV, Dang VXM. The effect of temperature and package to the quality and storage time of Hoa Loc mangoes. CTU J Sci. 2006; 6:9-17.

Duy LND, Ha NC, Lan LT, Tuyen NTK. Study on the use of chitosan to inhibit Colletotrichum gloeosporioides isolated from Cat Hoa Loc mango infected by anthracnose. CTU J Sci. 2014; 154-161.

EL Ghaouth A. Use of chitosan coating to reduce water loss and maintain quality of cucumber and bell pepper fruits. J Food Process Preserv. 1991; 15(5):359-368.

Shyu YS, Chen GW, Chiang SC, Sung WC. Effect of Chitosan and Fish Gelatin Coatings on Preventing the Deterioration and Preserving the Quality of Fresh-Cut Apples. Molecules. 2019; 24(10):2008.

Kim KM. Properties of chitosan films as a function of pH and solvent type. J Food Sci. 2006; 71(3):E119-E124.

Qiao C, Ma X, Wang X, Liu L. Structure and properties of chitosan films: Effect of the type of solvent acid. LWT. 2021; 135:109984.

Pen L and Jiang Y. Effects of chitosan coating on shelf life and quality of fresh-cut Chinese water chestnut. LWT. 2003; 36(3):359-364.

Zhang Z. β-Aminobutyric acid induces resistance of mango fruit to postharvest anthracnose caused by Colletotrichum gloeosporioides and enhances activity of fruit defense mechanisms. Sci Horticult. 2013; 160:78-84.

Ito A, Sato M, Anma T. Permeability of CO2 through chitosan membrane swollen by water vapor in feed gas. Die Angewandte Makromolekulare Chemie: Appl Macromol Chem Physics. 1997; 248(1):85-94.

Spangenberg JE and Vennemann TW. The stable hydrogen and oxygen isotope variation of water stored in polyethylene terephthalate (PET) bottles. Rapid Commun Mass Spectrom. 2008; 22(5):672-676.

Liu F. Novel starch/chitosan blending membrane: Antibacterial, permeable and mechanical properties. Carbohydr Polym. 2009; 78(1):146-150. Mima S. Highly deacetylated chitosan and its properties. J Appl Polym Sci. 1983; 28(6):1909-1917.

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Published

2024-06-29

How to Cite

Nguyen, T. D., Pham, Q. T., Nguyen, K. K., & Nguyen, T. N. (2024). Stability Study and Antifungal Activity of Chitosan Films from Shrimp Shells against Colletotrichum gloeosporioides. Tropical Journal of Natural Product Research (TJNPR), 8(6), 7345–7349. https://doi.org/10.26538/tjnpr/v8i6.2