The Immunostimulatory Effects of Peronema canescens. Jack Leaves Extract in Mus musculus L. Using the Carbon Clearance Method
DOI:
https://doi.org/10.26538/tjnpr/v8i3.5Keywords:
Carbon Clearance, Immunomodulator, Immunostimulant, Leukocytes, Peronema canescens JackAbstract
Sungkai leaf extract (Peronema canescens Jack.) has been used traditionally to cure various diseases. This research aimed to determine the immunostimulant effect of sungkai leaf extract using the carbon clearance method, the total leukocyte cell count, and the percentage of leukocyte cell types. Twenty-five male mice divided into 5 groups were used for this study. The extract was administered orally for 6 days at 25, 50, and 100 mg/kgbw, Na CMC 0.5% as negative control, and Stimuno 50 mg/kgbw as a comparison (positive control) group. The phagocytic index, total
leukocytes, and the percentage of leukocyte cell types were determined on the 7th day. The phagocytotic index value was analysed by one-way ANOVA followed by Duncan’s post-hoc test.
The increase in phagocytosis index showed that the effect of each dose was significantly different (p< 0.05) compared to the control group. The highest phagocytosis index was obtained from a 100 mg/kg bw dose. There was a significant difference (p<0.05) in the total leukocyte cell count and the percentage of leukocyte cell type. Sungkai leaf extract (Peronema canescens Jack.) had an immunostimulant effect on male white mice (Mus musculus L.).
References
Baratawidjaja KG RI. Basic Immunology Edition 11. Jakarta: Balai Penerbit Fakultas Kedokteran Indonesia; 2018.
Abbas K, Lichtman A PS. Basic Immunology : Function and Disorders of The Immune System. 5th Editio. Canada: Elsevier Inc; 2016.
Aldi Y, Rasyadi Y, Handayani D. Immunomodulatory Activity of Meniran Extracts (Phyllanthus niruri Linn.) on Broiler Chickens. J Sains Farm Klin. 2014;1(1):20–6.
Ibrahim A, Kuncoro H. Identification of Secondary Metabolites and Antibacterial Activity of Sungkai Leaf Extract (Peronema canescens Jack.) Against Several
Pathogenic Bacteria. J Trop Pharm Chem. 2012;2(1):8–18.
Ningsih, Arna dan Ibrahim A. Antimicrobial Activity of Sungkai Leaf (Peronema canescens Jack) n-Hexane Fraction Extract Against Some Bacteria Using TLC-Bioautography Method. J Trop Pharm Chem. 2013;2(2):76–82.
Andriani Fenny, Agus Sundaryono, Nurhamidah. Antiplasmodium Activity Test of the N-Hexane Fraction of Peronema Canescens Leaves Against Mus Musculus.
Alotrop. 2017;1(1):33–8.
Fransisca D, Kahanjak DN, Frethernety A. Test antibacterial activity of ethanol extract of sungkai leaves (Peronema canescens Jack.) on the growth of Escherichia coli using the Kirby-Bauer disc diffusion method. J Environ Sustain. 2020;4(1):460–70.
Yani AP. Potential Test of Sungkai Young Leaves (Peronema canescens) for Health (Immunity) in Mice (Mus Muculus). Pros Semin Biol. 2014;11(1):245–50.
RI Ministry of Health. Indonesian Herbal Pharmacopoeia. Edisi 2. Jakarta: Ministry of Health of The Republic of Indonesia; 2017.
Dillasamola D, Aldi Y, Kolobinti M. The effect of coriander ethanol extract (Coriandrum sativum L.) against phagocytosis activity and capacity of the macrophage cells and the percentage of leukocyte cells in white male mice. Pharmacogn J. 2019;11(6):1290–8.
Aldi Y, Dillasamola D, Florina T, Friardi D. Test immunomodulatory effects of ethanol extract skin of purple sweet potato (Ipomoea batatas (L.) Lam) with carbon clearance method and the number of leukocytes. Res J Pharm Biol Chem Sci. 2016;7(5):178–86.
Afriwardi, Aldi Y, Dillasamola D, Larakhansa YA, Badriyya E. Immunostimulatory activities of pegagan embun (Hydrocotyle sibthorpioides Lam.) in white male mice.
Pharmacogn J. 2021;13(2):368–75.
Andespal. Phytochemical Profiles of Sungkai Leaves (Peronema canescens) and Antioxidant and Cytotoxic Activity Tests of Sungkai Leaf Ethanol Extract. Universitas
Bengkulu; 2020.
Aldi Y, Oktavia S, Yenni B. Immunomodulatory Effect Test of Mangosteen Leaf Extract (Garcinia mangostana L.) Using Carbon Clearance Method and Counting Leukocyte Cell Counts in Male White Mice. J Farm Higea. 2016;8(1):20–31.
Alfitasari, D. A., Kusuma, A. M. and Hakim ZR. Immunomodulatory Activity of Shallot Ethanol Extract (Allium cepa L.) on Non-Specific Immune Response in
BALB/C Strain Male Mice with Carbon Clearance Method. Biosfera. 2016;34(2):75.
Aldi Y, Ogiana N, Handayani D. Immunomodulator Test of Several Subfractions of Meniran Ethyl Acetate Extract (Phyllanthus niruri L.) in Male White Mice Using Carbon Clearance Method. B-Dent, J Kedokt Gigi Univ Baiturrahmah. 2018;1(1):70–82.
Aldi Y, Husni E, Yesika R. Activity of kincung flowers (Etlingera Elatior Jack) on total leukocytes and percentage of leukocytes in allergic male white mice. Pharmacogn J. 2020;12(1):44–51.
Moyes, C. D. & PS. Principles of animal physiology. Vol. 754. San Francisco: Pearson/Benjamin Cummings.; 2008.
Aldi Y, Megaraswita, Dillasamola D. Effect of Elephantopus scaber linn. Leaf extract on mouse immune system. Trop J Pharm Res. 2019;18(10):2045–50.
Nugroho RA. Get to know mice as laboratory animals. Samarinda: Universitas Mulawarman; 2018.
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Tropical Journal of Natural Product Research (TJNPR)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
