Hepatoprotective Effect of Hydroalcoholic Extract of <i>Vitis vinifera</i> L Seeds on Paracetamol-Induced Liver Damage in Wistar Rats

Navenaa S.G. Pandian; Hemapriya Sankar; Sivakumar Ramalingam; Renuka Saravanan

doi:10.26538/tjnpr/v8i2.25

Authors

Navenaa S.G. Pandian Department of Chemistry and Biosciences, SASTRA Deemed to be University, Srinivasa Ramanujan Centre, Kumbakonam. 612 001, Tamil Nadu, India.
Hemapriya Sankar Department of Chemistry and Biosciences, SASTRA Deemed to be University, Srinivasa Ramanujan Centre, Kumbakonam. 612 001, Tamil Nadu, India.
Sivakumar Ramalingam Department of Chemistry and Biosciences, SASTRA Deemed to be University, Srinivasa Ramanujan Centre, Kumbakonam. 612 001, Tamil Nadu, India.
Renuka Saravanan Department of Chemistry and Biosciences, SASTRA Deemed to be University, Srinivasa Ramanujan Centre, Kumbakonam. 612 001, Tamil Nadu, India.

DOI:

https://doi.org/10.26538/tjnpr/v8i2.25

Keywords:

Antioxidant, Hepatoprotective,, Vitis vinifera L seed, Paracetamol

Abstract

Vitis vinifera L has been used traditionally in Pakistan, Italy, and Turkey as laxatives. Carminatives are used as drug therapy for many diseases. The study aims to determine the hepatoprotective potential of the hydroalcoholic extract of Vitis vinifera L (EVV) seeds in paracetamol-induced hepatotoxicity in albino rats. The hepatoprotective effect of the hydroalcoholic extractof Vitis vinifera L (EVV) seeds was tested in vivo by assessing the antioxidant parameters, liver enzymes, biochemical parameters, and histopathological examinations. Wistar rats-induced hepatotoxicity with paracetamol treated with Vitis vinifera L seeds at 200 and 400 mg/kg exhibited decrease in in vivo antioxidant parameters, liver enzymes, and biochemical parameters due to the presence of phytoconstituents such asflavonoids, steroids, glycosides, quinine, phenol, and saponin. The histological examinations of liver tissue revealed increased necrosis in untreated animals, whereas the central vein, surrounded by normal cells with good architecture, was observed in EVV-treated animals compared to silymarin-treated groups (25 mg/kg body weight). The scientific findings revealed that the hydroalcoholic extract of Vitis vinifera L. seed possesses hepatoprotective potential.

References

Nassiri-Asl M, Hosseinzadeh H. Review of the Pharmacological Effects of Vitis vinifera (Grape) and its Bioactive Constituents: An Update. Phytother Res. 2016 Sep;30(9):1392-403.

Singh J, Kaur H, Kaur R, Garg R, Prasad R, Assouguem A, Kara M, Bahhou J. A Review on the Nutritional Value and Health Benefits of Different Parts of Grape (Vitis viniferaL.). Trop. J. Nat. Prod. Res. 2023; 7(9):3874-3880.

Oraebosi MI and Elendu MU. Hepatoprotective Effect of Andropogongayanusagainst Paracetamol-and Carbon Tetrachloride Induced- Liver Toxicity in Rats.Trop. J. Nat. Prod. Res. 2021; 5(1):188-193.

Osna NA, Donohue TM Jr, Kharbanda KK. Alcoholic Liver Disease: Pathogenesis and Current Management. Alcohol Res. 2017;38(2):147-161

Mondal D, Das K, Chowdhury A. Epidemiology of Liver Diseases in India. Clin Liver Dis (Hoboken). 2022 Jan 28;19(3):114-117.

Rotundo L, Pyrsopoulos N. Liver injury induced by paracetamol and challenges associated with intentional and unintentional use. World J Hepatol. 2020; 12(4):125-136.

Edavalath, M. Ayurvedic dietary principles in prevention and management of Diabetes: A review. Curr Opin Endocrinol Diabetes Obes. 2018. 1(1), 2.

HarboneJB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 1st ed. London. Chapman and Hall; 1993. p. 27.

King J. In Practical Clinical Enzymology, Princeton MJ(Fol) Van D Nostrand Company, London. 1965.363.

Rosalki B, Donald Rau. Serum γ-glutamyl transpeptidase activity in alcoholism. Int J Clin Biochem Res.1972;39(1):41-7.

Folin O and Wu H. Determination of Glucose. J. Biol. Chem.1919. 38: 81. 08968851556.

Malloy HT and Evelyn KA. The determination of Bilirubin.J. Biol. Chem. 1937; 119:481.

Zlatkis, Zak, J Boyle. A new method for the direct determination of serum cholesterol. J. Lab. Clin. med.1953; Mar;41(3):486-92.

Foster L B, DunnR T. Stable reagents for determination of serum triglycerides by a colorimetric Hantzsch condensation method. Clin Chem.1973; 19(3):338-40.

Friedewald WT, Levy RI and Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma without the use of preparative centrifuge. Clin Chem.1972; 18: 499.

Lowry OH, Rosebrough NJ, Farr AL,Randal lRJ. Protein measurement with Folin Phenol Reagent. J.Biol.Chem.1951;193:265-275.

Ohkawa H, Ohishi N and Yagi K. Assay of lipid peroxides in animal tissues for thiobarbituric acid reaction.Annu. Rev. Biochem.1979; 95: 351-358.

Moron MS, Depierre JW, Mannervik B. “Levels of glutathione, glutathione reductase and glutathione S transferase activities in rat lung and liver”.Biochem and Biophys Acta. 1979; 5820:60-68.

Misra HP and FridovichI.The role of superoxide anion in the auto-oxidation of epinephrine and a simple assay for SOD. J. Biol. Chem. 1972; 247: 3170-3175.

Sinha Ak, “Colorimetric assay of catalase”. Anal Biochem. 1972.47:389-394.

Sinaga E, Fitrayadi A, Asrori A, Rahayu SE, Suprihatin S, Prasasty VD. Hepatoprotective effect of Pandanus odoratissimus seed extracts on paracetamol-induced rats. Pharm Biol. 2021; 59(1):31-39.

Zhou K, Raffoul JJ. Potential anticancer properties of grape antioxidants. J Oncol. 2012; 2012:803294.

SospeterNgoci Njeru, Josphat Matasyoh, Charles G. Mwaniki, Charles M. Mwendia, George Kimat Kobia. A Review of some Phytochemicals commonly found in Medicinal Plants. Int. J. Indig. Med. Plants. 105 (2013) 135-140.

Mujahid M, Hussain T, Siddiqui HH, Hussain A. Evaluation of hepatoprotective potential of Erythrina indica leaves against antitubercular drugs induced hepatotoxicity in experimental rats. J Ayurveda Integr Med. 2017; 8(1):7-12.

Shehu S, Abdurahman EM, Danmalam UH, Mohammed A, Shehu S, Iliyasu U.Effects of ThesiumvirideExtract and Fractions on Some Liver Biochemical Parameters in CCl4-Induced Damage in Wistar Rats.Trop. J. Nat. Prod. Res. 2022; 6(6):1004-1009.

Mujeeb M, Alam Khan S, Aeri V, Ali B. Hepatoprotective Activity of the Ethanolic Extract of Ficus caricaLinn. Leavesin Carbon Tetrachloride-Induced Hepatotoxicityin Rats. Iran J Pharm Res. 2011;10(2):301-306.

Mohammed N, Yaro AH, Nazifi AB. Evaluation of hepatoprotective activity of methanol stem bark extract of Haemato staphisbarteri Hook. F. against paracetamol and carbon tetrachloride-induced liver injury in rats. AJPT. 2017;6(2):88–95.

Liu L, Fu C, Li F. Acetate Affects the Process of Lipid Metabolism in Rabbit Liver, Skeletal Muscle and Adipose Tissue. Animals (Basel). 2019;9(10):799

Mahmoodzadeh Y, Mazani M, Rezagholizadeh L. Hepatoprotective effect of methanolic Tanacetum parthenium extract on CCl4-induced liver damage in rats. Toxicol Rep. 2017;4:455-462.

Laouar A, Klibet F, Bourogaa E, Benamara A, Boumendjel A, Chefrour A and Messarah M. Potential antioxidant properties and hepatoprotective effects of Juniperus phoenicea berries against CCl4 induced hepatic damage in rats. AsianPac. J. Trop. Med.2017; 10(3), 263-269.

Rotundo L, Pyrsopoulos N. Liver injury induced by paracetamol and challenges associated with intentional and unintentional use. World J Hepatol. 2020;12(4):125-136.

Mansourian M, Mirzaei A, Azarmehr N, Vakilpour H, Kokhdan EP, Doustimotlagh AH. Hepatoprotective and antioxidant activity of hydroalcoholic extract of Stachys pilifera. Benth on acetaminophen-induced liver toxicity in male rats. Heliyon. 2019;5(12):e03029.

Surai P F, Kochish I I, Fisinin V I, Kidd M T. Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update. Antioxidants. 2019; 8(7):235.

Chaudhary P, Janmeda P, Docea A O, Yeskaliyeva B, Abdull Razis A F, Modu B, Calina D, Sharifi-Rad, J. Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases. Front Chem. 2023;11:1158198.

Papackova Z, Heczkova M, Dankova H, Sticova E, Lodererova A, Bartonova L, Poruba M and Cahova M. Silymarin prevents acetaminophen-induced hepatotoxicity in mice. PLoS One. 2018;13(1):e0191353.