Analgesic and Antipyretic Activities of Ethanol Extract of Gardenia jasminoides Ellis Fruits in Mice


  • Tran T.P. Nhung Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam
  • Le P.T. Quoc Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam


Mus musculus, mice, antipyretic, analgesic, plant extract, Gardenia jasminoides


This study investigates the analgesic and antipyretic effects of Gardenia jasminoides Ellis fruit in mice. In vivo experiments were conducted using ethanol extract from Gardenia jasminoides fruit (EEGJ) at 75, 100, and 125 mg/kg. The positive control group was treated with standard medications, including aspirin (10 mg/kg) and fentanyl (10 mL/kg) for pain experiments, and acetaminophen (10 mg/kg) for fever experiments. In contrast, the negative control group received normal saline (10 mL/kg). The analgesic effectiveness of EEGJ was assessed by measuring response latency and the reduction in writhing responses. Fever was induced by injecting a 20% yeast suspension (10 mL/kg), and rectal temperature was measured both before and after the intervention. The results demonstrated that EEGJ significantly reduced acetic acid-induced writhing responses by 25.8%, 34.78%, and 48.27%, respectively. Furthermore, this extract exhibited a pronounced dose-dependent inhibition of formalin-induced pain during the second phase, with inhibition rates of 15.31%, 25.23%, and 33.57%, respectively. The highest pain inhibition due to mechanical stimulus in the Haffner tail clip test at 15 minutes was observed at a dose of 125 mg/kg, achieving an inhibition rate of 43.08%. Additionally, after 3 hours, rectal temperature significantly decreased in the EEGJ 125 mg/kg group (37.29 ± 0.12oC) (p < 0.05). In conclusion, our study unequivocally demonstrates that ethanol extract from Gardenia jasminoides fruit exhibits potent analgesic and antipyretic effects, with the highest activity observed at the dose of 125 mg/kg. Phytochemical constituents in EEGJ, such as alkaloids and flavonoids, may be responsible for the observed effects.


Bhowmick R, Sarwa MdS, Dewan SMR, Das A, Das B, Uddin MMN, Islam MdS, Islam MS. In vivo analgesic, antipyretic, and anti-inflammatory potential in Swiss albino mice and in vitro thrombolytic activity of hydroalcoholic extract from Litsea glutinosa leaves. Biol Res. 2014; 47(1): 56.

Hasan SMR, Hossain MM, Akter R, Jamila M, Mazumder MEH, Alam MA, Faruque A, Rana S, Rahman S. Analgesic activity of the different fractions of the aerial parts of Commelina benghalensis Linn. Int J Pharmacol. 2010; 6(1): 63-67.

Tesema S, Makonnen E. In vivo analgesic and antipyretic activities of n-butanol and water fractions of Ocimum suave aqueous leaves extract in mice. Ethiop J Health Sci. 2015; 25(2): 139-146.

Song JL, Wang R, Shi YP, Qi HY. Iridoids from the flowers of Gardenia jasminoides Ellis and their chemotaxonomic significance. Biochem Syst Ecol. 2014;56: 267-270.

Das BP, Ghosh A. Medicinal properties of phyto-constituents from Rubiaceae family of Assam: Critical Review. Int J Appl Chem Biol Sci. 2020; 1(3): 1-25.

Liu J, Yang C, Wu Z, Pei J, Chen Y, Huang X, Gao S, Kan R, Zhang W, Xie S, Fu X. Gardenia jasminoides Ellis fruit extracts attenuated colitis in 2,4,6-Trinitrobenzenesulfonic acid-induced rats. J Evidence-Based Complementary Altern Med. 2021; 2021: 9920379, 2021.

Banso A, Adeyemo S. Phytochemical screening and antimalarial assessment of Abutilon mauritianum, Bacopa monnifera and Datura stramonium. Biokemistri. 2006; 18(1): 39-44.

Joshi A, Bhobe M, Saatarkar A. Phytochemical investigation of the roots of Grewia microcos Linn. J Chem Pharm Res. 2013; 5(7): 80-87.

Chlopicka J, Pasko P, Gorinstein S, Jedryas A, Zagrodzki P. Total phenolic and total flavonoid content, antioxidant activity and sensory evaluation of pseudocereal breads. LWT – Food Sci Technol. 2012; 46(2): 548-555.

Stankovic MS. Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac J Sci. 2011; 33: 63-72.

Alison A. Basel Declaration defends animal research. Nature. 2010; 468: 742.

Livestock law in Vietnam, No. 32/2018/QH14. 2018, Ha Noi, Vietnam.

Guidelines for Preclinical and Clinical Trials of Oriental Medicines and Herbal Medicines, Decision 141/QD-K2DT. 2015. Ministry of Health, Ha Noi, Vietnam.

Akindele AJ, Oladimeji-Salami JA, Usuwah BA. Antinociceptive and anti-inflammatory activities of Telfairia occidentalis hydroethanolic leaf extract (Cucurbitaceae). J Med Food. 2015; 18(10): 1157-1163.

Chang CW, Chang WT, Liao JC, Chiu YJ, Hsieh MT, Peng WH, Lin YC. Analgesic and anti-inflammatory activities of methanol extract of Cissus repens in mice. J Evidence-Based Complementary Altern Med. 2012; 2012: 135379.

Surbakti BLB, Ginting CN, Chiuman L. Analgesic and antipyretic effects of red dragon fruit (Hylocereus polyrhizus) peel extract in white male rats. Tradit Med J. 2022; 27(3): 192-199.

Muhammad N, Saeed M, Khan H. Antipyretic, analgesic and anti-inflammatory activity of Viola betonicifolia whole plant. BMC Complementary Altern Med. 2012; 12: 59.

Iqbal E, Salim KA, Lim LBL. Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. J King Saud Univ Sci. 2015; 27(3): 224-232.

Kalita P, Tapan BK, Pal TK, Kalita R. Estimation of total flavonoids content (TFC) and antioxidant activities of 19.methanolic whole plant extract of Biophytum sensitivum Linn. J Drug Delivery Ther. 2013; 3(4), 33-37.

Devi BP, Boominathan R, Mandal SC. Evaluation of antipyretic potential of Cleome viscosa Linn (Capparidaceae) extract in rats. J Ethnopharmacol. 2003; 87(1): 11-13.

Safari VZ, Kamau JK, Nthiga PM, Ngugi MP, Orinda G, Njagi EM. Antipyretic, anti-inflammatory and analgesic activities of aqueous leaf extract of Aloe volkensii in albino mice. Med Aromat Plants. 2016; 5(3): 1000240.

Pentela B, Thalla S, Tharangini K, Geethanjali J, Govinda RT, Venkata LD. Hypoglycemic activity of ethanolic extract of Aphyllorchis montana on Alloxan induce diabetes in rats. Int J Chem Pharm Sci. 2012; 5(3): 27-29.

Satyanarayana PSV, Jain NK, Singh A, Kulkarni SK. Isobolographic analysis of interaction between cyclooxygenase inhibitors and tramadol in acetic acid-induced writhing in mice. Prog Neuro-Psychopharmacol Biol Psychiatry. 2004; 28(4): 641-649.

Abbott FV, Franklin KB, Ludwick RJ, Melzack R. Apparent lack of tolerance in the formalin test suggests different mechanisms formorphine analgesia in different types of pain. Pharmacol Biochem Behav. 1981; 15(4): 637-640.

Saha S, Guria T, Singha T, Maity TK. Evaluation of analgesic and anti-inflammatory activity of chloroform and methanol extracts of Centella asiatica Linn. Int Scholarly Res Not. 2013; 2013: 789613.

Chandrashekaran NV, Dai H, Roos KLT, Evanson NK, Tomsik J, Elton TS, Simmons DL. COX-3, a cyclooxygenase-1 variant inhibited by acetaminophen and other analgesic/antipyretic drugs: cloning structure and expression. Proc Natl Acad Sci U S A. 2002; 99(21): 13926-13931.



How to Cite

Nhung, T. T., & Quoc, L. P. (2023). Analgesic and Antipyretic Activities of Ethanol Extract of Gardenia jasminoides Ellis Fruits in Mice: Tropical Journal of Natural Product Research (TJNPR), 7(10), 4902–4907. Retrieved from

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