Investigations of Ameliorative Potentials Extract of P. pellucida on Salt - Fructose Induced Dyslipidemia in Wistar Rats
http://www.doi.org/10.26538/tjnpr/v7i9.40
Keywords:
antioxidant, phytochemical, vitamin, juice, watermelon, horned melon, Citrullus lanatus, Cucumis metuliferusAbstract
he study was undertaken to investigate the effect of extracts of P. pellucida on salt-fructose induced dyslipidemia in rats with a view to validating its consumption for the treatment and management of hyperlipidemia, obesity and high blood pressure. Dyslipidemia was induced in rats by oral administration of high concentrations of salt (NaCl, 18% (w/v)) and fructose (10%
(w/v) for 21 days. The induced rats (30) were randomly allotted into 5 groups of 6 rats per group . Test groups were treated consistently for 21 days with either 250 mg/kg bwt, 500 mg/kg bwt of Peperomia pellucida methanol extract (PPME) or standard drug (Atorvastatin, 0.6 mg/kgbwt). On day 22, the rats were anesthetized with diethyl ether and blood and liver collected for biochemical analyses. Plasma lipid profile parameters (Total cholesterol, LDL, HDL and VLDL cholesterol), atherogenic indices (atherogenic index, Coronary risk index I & II) and enzymatic / non-enzymatic antioxidants (Superoxide dismutase, Peroxidase and Glutathione), were evaluated using standard procedures. Results reveal significant increase in lipid profiles and metabolites, and significant decrease in the antioxidant enzymes of test animals compared with the control as a result of induction with high salt and fructose solution. However, the altered biochemical parameters were restored in the groups treated with PPME. The study suggests that P. pellucida may be of good value in the prevention and management of dyslipidaemia, a risk factor for cardiovascular diseases.
References
Rebecca LJ, Sharmila S, Das MP, Seshiah C. Extraction and purification of carotenoids from vegetables. J Chem & Pharm Res 2014; 6(4): 594-598.
Georgakouli K, Mpesios A, Kouretas D, Petrotos K, Mitsagga C, Giavasis I, Jamurtas AZ. The effects of an olive fruit polyphenol-enriched yogurt on body composition, blood redox status, physiological and metabolic parameters and yogurt microflora. Nutr 2016; 8(6): 344.
Khan N, Mukhtar H. Dietary agents for prevention and treatment of lung cancer. Cancer letters 2015; 359(2): 155- 164.
Stapleton PA, Goodwill AG, James ME, d’Audiffret AC, Frisbee JC. Differential impact of familial hypercholesterolemia and combined hyperlipidemia on vascular wall and network remodeling in mice. Microcirculation 2010; 17(1): 47-58.
Patil PD, Patil SP, Kelkar RK, Patil NP, Pise PV, Nadar SS. Enzyme-assisted supercritical fluid extraction: An integral approach to extract bioactive compounds. Trends in Food Sci & Techn 2021; 16: 357-369.
Azemi AK, Nordin ML, Hambali KA, Noralidin NA, Mokhtar SS, Rasool AH. Phytochemical contents and pharmacological potential of Parkia speciosa Hassk. for Diabetic Vasculopathy: A Review. Antioxidants 2022; 11(2): 431.
Adegbola P, Aderibigbe I, Hammed W, Omotayo T. Antioxidant and anti-inflammatory medicinal plants have potential role in the treatment of cardiovascular disease: a review. Am J Cardiovasc Disease 2017; 7(2): 19.
Kumar Singh A, Cabral C, Kumar R, Ganguly R, Kumar Rana H, Gupta A, Pandey AK. Beneficial effects of dietary polyphenols on gut microbiota and strategies to improve delivery efficiency. Nutr 2019; 11(9): 2216.
Viswanatha GL, Vaidya SK, Ramesh C, Krishnadas N, Rangappa S. Antioxidant and antimutagenic activities of bark extract of Terminalia arjuna. Asian Pac J Trop Med 2010; 3(12): 965-970.
Riglar DT, Giessen TW, Baym M, Kerns SJ, Niederhuber MJ, Bronson RT, Silver PA. Engineered bacteria can function in the mammalian gut long-term as live diagnostics of inflammation. Nature Biotech 2017; 35(7): 653-658.
Fakayode AE, Imaghodor FI, Fajobi AO, Emma-Okon BO, Oyedapo OO. Phytonutrients, antioxidants and antiinflammatory analysis of Peperomia pellucida. J Med Pharma & Appl Sci 202; 10(15): 3517-3523.
Oyedapo O, Amos S. Further investigations into the bioactivity of root extract of Plumbago zeylanica. Phyt Res 1997: 11(1): 62-63.
Mainieri F, La Bella S, Chiarelli F. Hyperlipidemia and Cardiovascular Risk in Children and Adolescents. Biomed 2023; 11(3): 809.
Expert Panel on Detection E. Executive summary of the third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (Adult Treatment Panel III). Jama. 2001 May 16; 285(19): 2486-97.
Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974; 47(3): 469-474.
Reddy KP, Subbani SM., Khan PA, Kumar KB. Effect of light and benzyl adenine in dark trated growing leaves, changes in the peroxidase activity. Cell Physiol 1995; 29: 984.
Moron MS, Depierre JW, Manneryik B. Levels of glutathione, glutathione reductase and glutathione-Stransferase activities in rat lung and liver. Biophys Acta 1979; 582: 67-78.
Do R, Willer CJ, Schmidt EM, Sengupta S, Gao C, Peloso GM, Chen J. Common variants associated with plasma triglycerides and risk for coronary artery disease. Nature Genetics 2013; 45(11): 1345-1352.
Dubois M, Gilles K, Hamilton J, Rebers P, Smith F. A colorimetric method for the determination of sugars. Nature 1951; 168: 167-167.
Ye J, DeBose-Boyd RA. Regulation of cholesterol and fatty acid synthesis. Cold Spring Harbor Perspectives in Biology
; 3(7): a004754.
Dashty M, Motazacker MM, Levels J, de Vries M, Mahmoudi M, Peppelenbosch MP, Rezaee F. Proteome of human plasma very low-density lipoprotein and low-density lipoprotein exhibits a link with coagulation and lipid metabolism. Throm and Haem 2014; 112(03): 518-530.
Ivanova EA, Myasoedova VA, Melnichenko AA, Grechko AV, Orekhov AN. Small dense low-density lipoprotein as biomarker for atherosclerotic diseases. Oxid Med Cell Longev 2017; 2017: 1273042.
Zhu AX, Finn RS, Edeline J, Cattan S, Ogasawara S, Palmer D, Vogel A. Pembrolizumab in patients with advanced hepatocellular carcinoma previously treated with sorafenib (KEYNOTE-224): a non-randomised, open-label phase 2 trial. The Lancet Oncology 2018; 19(7): 940-952.
Akbas EM, Timuroglu A, Ozcicek A, Ozcicek F, Demirtas L, Gungor A, Akbas N. Association of uric acid, atherogenic index of plasma and albuminuria in diabetes mellitus. Inter J Clin Exp Med 2014; 7(12): 5737.
obi sov M, rohlich J. The plasma parameter log (TG/HDL-C) as an atherogenic index: correlation with lipoprotein particle size and esterification rate inapoblipoprotein-depleted plasma (FERHDL). Clin Biochem 2001; 34(7): 583-588.
Egli L, Lecoultre V, Cros J, Rosset R, Marques AS, Schneiter P, Tappy L. Exercise performed immediately after fructose ingestion enhances fructose oxidation and suppresses fructose storage. The Am J clin Nutr 2016; 103(2): 348-355.
Yokozawa T, Yamabe N, Kim HY, Kang KS, Hur JM, Park CH, Tanaka T. Protective effects of morroniside isolated from Corni Fructus against renal damage in streptozotocininduced diabetic rats. Biol Pharm Bull 2008; 31(7): 1422- 1428.
Elliott SS, Keim NL, Stern JS, Teff K, Havel PJ. Fructose, weight gain, and the insulin resistance syndrome. The Am J Clin Nutr 2002; 76(5): 911-922.
Geidl-Flueck B, Hochuli M, Németh Á, Eberl A, Derron N, Köfeler HC, Gerber PA. Fructose-and sucrose-but not glucose-sweetened beverages promote hepatic de novo lipogenesis: A randomized controlled trial. J Hep 2021; 75(1): 46-54.
Abere T, Okpalaonyagu S. Pharmacognostic evaluation and antisickling activity of the leaves of Peperomia pellucida (L.) HBK (Piperaceae). Afr J Pharm Pharm 2015; 9(21): 561-566.
Oloyede GK, Onocha PA, Olaniran BB. Phytochemical, toxicity, antimicrobial and antioxidant screening of leaf extracts of Peperomia pellucida from Nigeria. Adv Env Biol 2011; 5(12): 3700-3709.
Raghavendra H, Kekuda P, Chethan D. Phytochemical analysis and in vitro antioxidant activity of Rubus apetalus Poir.(Rosaceae). Pharm 2018; 1: 187-195.
Zubair K, Samiya J, Jalal U, Mostafizur R. In vitro investigation of antdiarrhoeal, antimicrobial and thrombolytic activities of aerial parts of Peperomia pellucida. Pharm 2015; 3: 5-13.
Adaramoye OA, Akintayo O, Achem J, Fafunso MA. Lipid-lowering effects of methanolic extract of Vernonia amygdalina leaves in rats fed on high cholesterol diet. Vasc Health Risk Manag 2008; 4(1): 235-241.
Duangjai A, Limpeanchob N, Trisat K, Ounaroon A. Effects of extraction solvents of dietary plants on lipid lowering activity. J Med Plants Res 2016; 10(32): 538-545.
Imam MU, Ishaka A, Ooi DJ, Zamri NDM, Sarega N, Ismail M, Esa NM. Germinated brown rice regulates hepatic cholesterol metabolism and cardiovascular disease risk in hypercholesterolaemic rats. J Functional Foods 2014; 8: 193-203.
Grossman S, Dovrat S, Bergman M. Natural antioxidants: just free radical scavengers or much more? Trends in Cancer Res 2011; 7: 57-73.
Goiris K, Muylaert K, Fraeye I, Foubert I, De Brabanter J, De Cooman L. Antioxidant potential of microalgae in relation to their phenolic and carotenoid content. J Appl Phycol 2012; 24: 1477-1486.
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