Activities of <i>Hydrocotyle sibthorpioides</i> Lam. Extract in Capsule on Natural Killer and CD8 Cells in Human
http://www.doi.org/10.26538/tjnpr/v7i8.29
Keywords:
NK cell, Immunostimulant, Hydrocotyle sibthorpioides Lam., Clinical trial, CD8 cellAbstract
Hydrocotyle sibthorpioides Lam. has been used by Indonesians to increase immunity. The present study was therefore conducted to investigate the effect of Hydrocotile sibthorpioides Lam. extract in capsules on the activity of natural killer (NK) and CD8 cells in humans during the first phase of clinical trials. Twenty participants were recruited for the study and divided into two groups. The first group received nothing except an additional ingredient as a placebo. Also, the second group was administered a preparation test at a dose of 67 mg per person. Blood was obtained before and after the test preparation was administered, which was done once daily for three days. The protein concentrations of NK and CD8 cells were measured by the enzyme-linked immunosorbent assay (ELISA) kit. The results showed that the average activity of NK cells before giving the preparations to the first group was 81.644 U/mL, and after administration was 81.910 U/mL. In contrast, the average activity of NK cells of the second group was 81.998 and 83.541U/mL, respectively. The concentrations of CD8 cells in the first group were 230.160 and 235.575U/mL before and after administration, respectively. Compared to the second group, the concentrations of CD8 cells were 238.407 and 335.323 U/mL, respectively. The findings of this study indicate that the H. sibthorpioides extract could increase the NK and CD8 cellular activities, which may improve the body's capacity to destroy virus invaders.
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