De Novo Whole Genome Sequencing Data of Pseudomonas aeruginosa ATCC10145, an Opportunistic Pathogen
doi.org/10.26538/tjnpr/v6i2.2
Keywords:
Whole genome sequencing, Pseudomonas aeruginosa, Genome assembly, Virulence factorAbstract
Pseudomonas aeruginosa is an opportunistic pathogen that is commonly found in nosocomial infections. P. aeruginosa is one of the most frequent model bacterial species, with the genomes of hundreds of strains of this species have been sequenced to date. This study aimed to analyze the whole genome sequence of P. aeruginosa ATCC® 10145TM . The whole genome of the P. aeruginosa ATCC® 10145TMwas sequenced by shotgun sequencing using Genomic DNA Mini Kit, genome AnalyzerIIX with 100-bp paired-end reads and genomic DNA extracted using the MasterPure complete DNA and RNA purification kit and complete genome sequence analysis was done. The genome of the P. aeruginosa ATCC® 10145TM was sequenced on the IlluminaMiseq platform. The raw sequenced reads were assessed for quality using FastQC v.0.11.5 and filtered for low quality reads and adapter regions using Trimmomatic v.0.36. The de novo genome assembly was made with CLC Genomics Workbench 5.1 and annotated using Prokaryotic Genome Annotation Pipeline (PGAP) v4.10. Here, we report the whole genome sequence of P. aeruginosa ATCC® 10145TM strain. All filtered and assembled genomic data sequences have been submitted to National Centre for Biotechnology Information (NCBI) and can be located at DDBJ/ENA/GenBank under the accession of VAOQ00000000 (UniSZA) and BioProject number PRJNA533327 and ID: 533327). The high-quality P.aeruginosa ATCC® 10145TM genome sequence provides a reference for further research including investigation of horizontal gene transfer or comparative genomics.
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