Development of HPLC-Based QuEChERS Method for High Recovery of Fungicides from Vegetable Samples
doi.org/10.26538/tjnpr/v6i2.1
Keywords:
Fenugreek, Fungicides, HPLC, QuEChERS, Recovery, Spinach, VegetablesAbstract
Vegetables are the most essential part of our diet. Several fungicides are used in controlling pathogens in vegetable plants. The control of phytopathogens with fungicides may be associated with many health hazards. Therefore, the present study was conducted to develop a simple high performance liquid chromatography (HPLC)-based Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method for the recovery of fungicides (azoxystrobin and thiophenate methyl) from vegetables. Two types of vegetables; spinach and fenugreek used for this study were crushed, homogenized, and treated with acetonitrile in a centrifuge tube. Sodium sulphate and sodium chloride were added to maintain the pH of the sample solution and acetonitrile was added to remove the matrix by a clean-out method using dispersive solid-phase extraction (dSPE). Finally, HPLC analysis was carried out to determine the test fungicides in the two vegetable samples. The results indicated that recoveries of the test fungicides were in the range of 0.63 to 107.68% at spiking levels and different factors through percentage RSD between 3.6 and 4.7%. The limit of detection (LOD) and limit of quantification (LOQ) of the two fungicides were in the range of 0.0021 to 0.00181 mg/kg and 0.0706 to 0.00606 mg/kg, respectively. In the QuEChERS method, the different concentrations of the fungicides in the spinach vegetable were obtained, but in the fenugreek vegetable, these pesticides were absent. The findings of this study have shown that the proposed method is fast, easy, and efficient for fungicide analysis with high recovery, low limit of detection, and limit of quantification.
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