Anti-Hyperglycemic and Anti-Inflammatory Activities of Moringa oleifera Lam Leaves Extract

doi.org/10.26538/tjnpr/v6i6.10

Authors

  • Jessica Novia Department of Pharmacy, Universitas Pelita Harapan, Jl. M.H. Thamrin Boulevard, Tangerang 15811, Indonesia
  • Denny Juvi Department of Pharmacy, Universitas Pelita Harapan, Jl. M.H. Thamrin Boulevard, Tangerang 15811, Indonesia
  • Riskianto Riskianto Department of Pharmacy, Universitas Pelita Harapan, Jl. M.H. Thamrin Boulevard, Tangerang 15811, Indonesia
  • Marcelia Sugata Department of Biology, Universitas Pelita Harapan, Jl. M.H. Thamrin Boulevard, Tangerang 15811, Indonesia
  • Dela Rosa Department of Pharmacy, Universitas Pelita Harapan, Jl. M.H. Thamrin Boulevard, Tangerang 15811, Indonesia

Keywords:

Alpha glucosidase, Alpha amylase, Anti-inflammatory, Anti-hyperglycemic, Moringa oleifera

Abstract

Moringa oleifera has been known for its nutritional content and health benefit. As a medicinal plant, it has been used to control and treat diabetes with minimal or no side effects. Hence, this study investigated the anti-hyperglycemic and anti-inflammatory activity of Moringa leaf extract (MLE). Extracts were obtained by maceration with 1 L of 70% ethanol, ethyl acetate, and n hexane. Anti-hyperglycemic activity of MLE was evaluated through alpha-amylase and alpha glucosidase inhibition, while anti-inflammatory activity was analyzed by protein denaturation method. The results showed that ethyl acetate extract of Moringa leaves had the strongest inhibition against alpha-amylase (IC50 = 0.243 g/mL) and alpha-glucosidase (IC50 = 0.470 g/mL). Meanwhile, the greatest anti-inflammatory activity (IC50 = 0.217 g/mL) was shown by n-hexane extract of Moringa leaves. In conclusion, Moringa leaves have anti-hyperglycemic and anti-inflammatory activities. 

References

Banerjee SK and Maulik SK. Effect of garlic on cardiovascular disorders: a review. Nutr J. 2002; 1(1):4.

American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2012; 35(Suppl 1):S64-S71

Punthakee Z, Goldenberg R, Katz P. Definition, classification and diagnosis of diabetes, prediabetes and metabolic syndrome. Can J Diabetes. 2018; 42(1):S10-S15.

Mani S and Vadivambal JSR. Optimization of solvent extraction of Moringa (Moringa oleifera) seed kernel oil using Response Surface Methodology. Food Bioprod Proc. 2007; 85(C4):328-335.

Guillen A, Granados S, Rivas KE, Estrada O, Echeverri LF, Balcazar N. Antihyperglycemic activity of Eucalyptus tereticornis in insulin-resistant cells and a nutritional model of diabetic mice. Adv Pharmacol Sci. 2015; 2015(1):2-10.

Ministry of Health Republic of Indonesia. Situasidananalisis diabetes. [Online]. 2014 [Cited 2021 Aug 10]. Available from https://www.kemkes.go.id/article/view/15021800007/situasi-dan-analisis-diabetes.html.

Ministry of Health Republic of Indonesia. Tahun 2030 prevalensi diabetes melitus di Indonesia mencapai 21,3 juta orang. [Online]. 2009. [Cited 2021 Aug 10]. Available from: https://www.kemkes.go.id/article/view/414/tahun-2030-prevalensi-diabetes-melitus-di-indonesia-mencapai-213-juta-orang.htmL.

Dipiro JT, Wells BG, Schwinghammer TL, DiPiro CV. Pharmacotherapy Handbook. (9th ed.) UK: McGraw-Hill Education Companies; 2015; 169-170p.

Gupta RK, Kesari AN, Murthy PS, Chandra R, Tandon V, Watal G. Hypoglycemic and antidiabetic effect of ethanolic extract of leaves of Annona squamosa L. in experimental animals. J Ethnopharmacol. 2005; 99(1):75-81.

Balamurugan K, Nishanthini A, Mohan VR. Antidiabetic and antihyperlipidemic activity of ethanol extract of Melastomamalabathricum Linn. leaf in alloxan induced diabetic rats. Asian Pac J Trop Biomed. 2014; 4:S442-S448.

Patel AB, Prajapati DD, Patel Y. Antidiabetic activity of Moringa oleifera Lam. Algerian J Nat Prod. 2017; 5(2):446-453.

Yajnik CS. The insulin resistance epidemic in India: fetal origins, later lifestyle, or both. Nutr Rev. 2001; 59(1):1-9.

Chumark P, Khunawat P, Sanvarinda Y, Phornchirasilp S, Morales NP, Phivthong-Ngam L, Ratanachamnong P, Srisawat S, Pongrapeeporn KU. The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves. J Ethnopharmacol. 2008; 116(3):439–446.

Rizkayanti R, Diah AWM, Jura MR. Antioxidant activity tests of water and ethanol extracts of Moringa (Moringa oleifera LAM) Leaves. J Akad Kim. 2017; 6(2):125-131.

Vergara-Jimenez M, Almatrafi MM, Fernandez ML. Bioactive Components in Moringa Oleifera Leaves Protect against Chronic Disease. Antioxidant (Basel). 2017; 6(4):91.

Parvathy MVS and Umamaheshwari A. Cytotoxic effect of Moringa oleifera leaf extracts on human multiple myeloma cell lines. Trends Med Res. 2007; 2(1):44-50.

Moyo B, Masika PJ, Muchenje V. Antimicrobial activities of Moringa oleifera Lam leaf extracts. Afr J Biotechnol. 2012; 11(11):2797-2802.

Riskianto R, Kamal SE, Aris M. Antioxidant activity of 70% ethanol extract of Moringa (Moringa oleifera LAM) leaves on DPPH. J Pro-Life. 2021; 8(2):168-177.

Cheenpracha S, Park EJ, Yoshida WY, Barit C, Wall M, Pezzuto JM, Chang LC. Potential anti-inflammatory phenolic glycosides from the medicinal plant Moringa oleifera fruits. Bioorg Med Chem. 2012; 18(17):6598–6602.

Sudha P, Syed S, Asdaq SMB, Dhamingi S, Chandrakala GK. Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in animals. Indian J Physiol Pharmacol. 2010; 54(2):133–140.

Buraimoh AA, Bako IG, Ibrahim FB. Hepatoprotective effect of ethanolic leave extract of Moringa oleifera on the histology of paracetamol induced liver damage in Wistar rats. Int J Anim Vet Adv. 2011; 3(1):10-13.

Goyal BR, Agrawal BB, Goyal RK, Mehta AA. Phytopharmacology of Moringa oleifera Lam. An overview. Nat Prod Radian. 2007; 6(4):347–353.

Kasolo JN, Bimenya GS, Ojok L, Ochieng J, Ogwal-Okeng JW. Phytochemicals and uses of Moringa oleifera leaves in Ugandan rural communities. J Med Plants Res. 2010; 4(9):753-757.

Madukwe EU, Ugwuoke AL, Ezeugwu JO. Effectiveness of dry Moringa oleifera leaf powder in treatment of anemia. University of Nigeria Nsukka, Enugu State, Nigeria: Department of Home Science, Nutrition and Dietetics; 2013; 5(5):226-228.

Leone A, Spada A, Battezzati A, Schiraldi A, Aristil J, Bertoli S. Cultivation, Genetic, Ethnopharmacology, Phytochemistry and Pharmacology of Moringa oleifera Leaves: An Overview. Int J Mol Sci. 2015; 16(6):12791-12835.

Kazeem MI, Adamson JO, Ogunwande IA. Modes of Inhibition of -Amylase and -Glucosidase by Aqueous Extract of Morinda lucida Benth Leaf. BioMed Res Int. 2013; 527570.

Telagari M and Hullati K. In-vitro -amylase and -glucosidase inhibitory activity of Adiantum caudatum Linn. and Celosia argenta Linn. extracts and fractions. Indian J Pharmacol. 2015; 47(4):425-429.

Gunathilake KDPP, Ranaweera KKDS, Rupasinghe HPV. In Vitro Anti-Inflammatory Properties of Selected Green Leafy Vegetables. Biomed. 2018; 6(4):107.

Hollander P, Pi-Sunyer X, Coniff RF. Acarbose in the treatment of type I diabetes. Diabetes Care. 1997; 20(3):248-253.

Gupta R, Mathur M, Bajaj VK, Katariya P, Yadav S, Kamal R, Gupta RS. Evaluation of antidiabetic and antioxidant activity of Moringa oleifera in experimental diabetes. J Diabetes. 2012; 4(2):164-171.

Khan W, Parveen R, Chester K, Parveen S, Ahmad S. Hypoglycemic potential of aqueous extract of Moringa oleifera leaf and in vivo GC-MS metabolomics. Front Pharmacol. 2017; 8(1):577.

Patel DK, Kumar R, Laloo D, Hemalatha S. Diabetes mellitus: an overview on its pharmacological aspects and reported medicinal plants having antidiabetic activity. AsianPac J Trop Biomed. 2012; 2(5):411-420.

Mayur BS, Sandesh S, Shruti S, Sung-Yum. Antioxidant and a-glucosidase inhibitory properties of Carpesium abrotanoides L. J Med Plant Res. 2010; 4(15):1547-1553.

Amarowicz R. Tannins: the new natural antioxidants?. Eur J Lipid Sci Technol. 2007; 109:549-551.

Song YJE, Manson JE, Buring D, Howard S, Liu. Associations of dietary flavonoids with risk of type 2diabetes and markers of insulin resistance and systemic inflammation in women: A prospective study and crosssectional analysis. J Am Coll Nutr. 2005; 24(5):376-384.

Frankel EN and Meyer AS. The problems of using onedimensional methods to evaluate multifunctional food and biological antioxidants. J Sci Food Agric. 2000; 80(13):1925-1941.

Minaiyan M, Asghari G, Taheri D, Saeidi M, Nasr-Esfahani S. Anti-inflammatory effect of Moringa oleifera Lam. Seeds on acetic acid-induced acute colitis in rats. AvicennaJ Phytomed. 2014; 4(2):127-136.

Maisuthisakul P, Suttajit M, Pongsawatmanit R. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chem. 2007; 100(4):1409-1418.

Pawate S, Shen Q, Fan F, Bhat NR. Redox regulation of glial inflammatory response to lipopolysaccharide and interferon. J Neurosci Res. 2004; 77(4):540-551.

Ghosh S, Hayden M. New regulators of NF-B in inflammation. Nat Rev Immunol. 2008; 8(11):837-848.

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Published

2022-06-01

How to Cite

Novia, J., Juvi, D., Riskianto, R., Sugata, M., & Rosa, D. (2022). Anti-Hyperglycemic and Anti-Inflammatory Activities of Moringa oleifera Lam Leaves Extract: doi.org/10.26538/tjnpr/v6i6.10. Tropical Journal of Natural Product Research (TJNPR), 6(6), 884–888. Retrieved from https://www.tjnpr.org/index.php/home/article/view/1496

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Vol. 6 No. 6 (2022): Tropical Journal of Natural Product Research

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