Preliminary Phytochemical Screening and GC-MS Analysis of Ethanol Extract of Bulbs of Eleutherine sp.

doi.org/10.26538/tjnpr/v6i3.10

Authors

  • Aditya Fridayanti Pharmaceutical Sciences Doctoral Prog, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia
  • Djoko A. Purwanto Pharmaceutical Sciences Doctoral Prog, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia
  • Esti Hendradi Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia

Keywords:

Eleutherine sp., GC-MS analysis, Phytochemical screening, Ethanol extract.

Abstract

The Dayak community is known to use Eleutherine palmifolia as a wound and ulcer treatment. However, according to published research, other species of Eleutherine possess wound healing properties. In addition, the active compound components of species Eleutherine induce antibacterial and antioxidant bioactivity which can aid the healing process. As a result, the aim of this research is to identify active components in an ethanol extract of Eleutherine sp bulb obtained through maceration with 70% ethanol as the solvent. Chemical compounds were analyzed using GC-MS, while the mass spectra of compounds found in extracts was compared to those contained in the National Institute of Standards and Technology (NIST) library. Preliminary phytochemical screening revealed the presence of bioactive compounds such as triterpenoids, steroids, and tannins. The mass spectral measurements revealed 13 peaks, indicating the existence of that number of active phytochemical components including 1H-Indene, 2-butyl-5-hexyloctahydro; Benzene, 1,2,3-trimethyl; and Phenol, 2,5-bis (1,1-dimethylethyl). It is intended that the findings of this study will provide more information about the active compound components of Eleutherine sp. which promote biological activity that aids wound healing. 

References

Harlita TD, Oedjijono, Asnani A. The Antibacterial Activity of Dayak Onion (Eleutherine palmifolia (L.) Merr) towards

Pathogenic Bacteria. Trop Life Sci Res. 2018; 29(2):39-52.

Warsiti, Sisca DKW, Ardea AR, Ratna Y. Antibacterial Activity of Ethanolic Extract of Eleutherine palmifolia (L.) Merr against Bacteria Staphylococcus aureus. Pharm J Indones. 2018; 15(2):75-82.

Upadhyay A, Chattopadhyay P, Goyary D, Mazumder PM, Veer V. Eleutherine indica L. accelerates in vivo cutaneous wound healing by stimulating Smad-mediated collagen production. J Ethnopharmacol. 2013; 146(2):490-494.

Villegas LF, Fernández ID, Maldonado H, Torres R, Zavaleta A, Vaisberg AJ, Hammond GB. Evaluation of the wound-healing activity of selected traditional medicinal plants from Peru. J Ethnopharmacol. 1997; 55(3):193-200.

Kuntorini EM, Astuti MD, Misrina. Anatomical structure and antioxidant activity of red bulb plant (Eleutherine mericana) on different plant age. biodiversitas. 2016; 17(1):229-233.

Song SH, Min HY, Han AR, Nam JW, Seo EK, Seoung Woo Park, Sang Hyung Lee, Sang Kook Lee. Suppression of inducible nitric oxide synthase by (-)-isoeleutherin from the bulbs of Eleutherine americana through the regulation of NF-kappaB activity. Int Immunopharmacol. 2009; 9(3):298-302.

Ifesan BO, Hamtasin C, Mahabusarakam W, Voravuthikunchai SP. Inhibitory effect of Eleutherine americana Merr. extract on Staphylococcus aureus isolated from food. J Food Sci. 2009; 74(1):31-36.

Hong Van NT, Nguyet Que DT, Hang NT, Loan LT, Anh LT, Hoan NV. Study on antibiotics activity in vitro and treatment

effects in place in the experimental burns of EB product isolated from the rhizome of Eleutherine bulbosa. Acad J Biol. 2014; 36(1):81-87.

Fridayanti A, Sastyarina Y, Herman H, Rahmadani A, Firmansyah G, Widyanti TW, Nur Y, Kuncoro H, Wijayanti E. Standarisasi Ekstrak Umbi Bawang Tiwai (Eleutherine americana (Aubl.) Merr.) Asal Kalimantan Timur. Proc Mul Pharm Conf. 2017; 6(1):90-97.

Rani VS and Nair BR. GC-MS Analysis of Ethyl Acetate Extract of Eleutherine Bulbosa (Urban) Miller (Iridaceae). Int J Pharm Sci Res. 2016;7(4):1729-1733.

Subramaniam K, Suriyamoorthy S, Wahab F, Sharon FB, Rex GR. Antagonistic activity of Eleutherine palmifolia Linn. Asian Pac J Trop Dis. 2012; 2(1):491-493.

Fauziati, Sampepana E, Kurniawaty. Characteristic of active compounds on extraction of fresh and dry Tiwai onions using ethanol solvent. Proceedings of the 1st National Seminar. 2017; 1:302-307.

Munaeni W, Widanarni, Yuhana M, Setiawati M, Wahyudi AT. Phytochemical analysis and antibacterial activities of Eleutherine bulbosa (Mill.) Urb. extract against Vibrio parahaemolyticus. Asian Pac J Trop Biomed. 2019; 9(9):397-404.

Rahmi M, Helmina W, Wahyudin BJ, Kartini, Finna S, Muhamad F, Abdul W. Standardization of Eleutherine bulbosa Urb. Bulbs and Total Flavonoid Content from Three Locations in Kalimantan, Indonesia. Pharmacogn J. 2021; 13(1):73-80.

MojabF, Kamalinejad M, Ghaderi N, Vahidipour HR.Phytochemical screening of some species of Iranian Plants.

Iran J Pharm Sci. 2003; 2:77-82.

Ghosal M, Mandal P. Phytochemical screening and antioxidant activities of two selected ‘Bihi’ fruits used as vegetables in Darjeeling Himalaya. Int. J. Pharm.2012; 4(2):567-574.

Chaudhary P, Ahmad S, Khan NA. Isolation and Characterization of Volatile oil by GC-MS Analysis and Screening of In Vitro Antioxidant Potential of Hydroalcoholic Extracts of Some Indigenous Plants of Northern India in Rodents. Biomed

Pharmacol J. 2019; 12(4):1867-1880.

Ombuna DH, Ruth NW, Hudson N, Ahmed H. Anti-bacterial properties and GC-MS analysis of extracts and essential oils of selected plant product. Biofarmasi J Nat Prod Biochem. 2018; 16(1):44-58.

Nuhu I, Adamu HM, Abubakar SU, Shibdawa MA, Babbai, M. GC-MS And IR Analysis Of Ethylacetate. Roots Extracts Of Anogeissus leiocarpus. Int J Sci Eng. 2017; 8(3):115-120.

Nurlybekova A, Ye Y, Abilov Z, Jenis J. Determination of chemical composition of the Ligularia narynensis root by gas

chromatography-mass spectrometry. Chem Bull Kaz Nat Univ. 2019; 95(4):14-19.

Peng DQ, Yu ZX, Wang CH, Gong B, Liu YY, Wei JH. Chemical Constituents and Anti-Inflammatory Effect of Incense Smoke from Agarwood Determined by GC-MS. International Journal of Analytical Chemistry. 2020; 1-19.

Wang J, Karthikeyan S, Liu H, Cai J. Natural antiphytopathogenic fungi compound phenol, 2, 4-bis (1, 1-dimethylethyl) from Pseudomonas fluorescens TL-1 Jianguo Ren1, 2. Indian J Biochem. 2019; 56(2):162-168.

Rangra, NK, Samanta,S, Pradhan, KK. In vivo Anti-inflammatory Potential of Leaf Extracts of Acacia auriculiformisBenth. Indian J Pharm Sci. 2019; 81(4):709-719.

Hussain AZ, Kumaresan S. GC-MS analysis and antibacterial evaluation of Acalypha indica. Asian J Plant Sci Res. 2013;

(6):46-49.

Huang L, Zhu X, Zhou S, Cheng Z, Shi K, Zhang C, Shao H. Phthalic Acid Esters: Natural Sources and Biological

Activities. Toxins 2021; 13(7):1-17.

Koyama S, Purk A, Kaur M, Soini HA, Novotny MV, Davis K, Kao CC, Matsunami H, Mescher A. Beta-caryophyllene enhances wound healing through multiple routes. PLoS One. 2019;14(12):1-32.

Parisotto-Peterle J, Bidone J, Lucca LG, Araújo GMS, Falkembach MC, da Silva Marques M, Horn AP, Dos Santos MK,

da Veiga VF Jr, Limberger RP, Teixeira HF, Dora CL, Koester LS. Healing activity of hydrogel containing nanoemulsified β-

caryophyllene. Eur J Pharm Sci. 2020; 148:1-12.

Downloads

Published

2022-05-01

How to Cite

Fridayanti, A., A. Purwanto, D., & Hendradi, E. (2022). Preliminary Phytochemical Screening and GC-MS Analysis of Ethanol Extract of Bulbs of Eleutherine sp.: doi.org/10.26538/tjnpr/v6i3.10. Tropical Journal of Natural Product Research (TJNPR), 6(3), 361–364. Retrieved from https://www.tjnpr.org/index.php/home/article/view/134

Most read articles by the same author(s)