Assessment of Acute Toxicity, LD50 and Histopathological Evaluation of Nigella sativa and Moringa oleifera Seed Extracts in Wistar Rats
http://www.doi.org/10.26538/tjnpr/v6i11.15
Keywords:
Rats, Hepatotoxicity, Acute toxicity, Nigella sativa, Moringa oleiferaAbstract
The study determined the oral LD50 value of n-hexane oil extracts of Nigella sativa and Moringa oleifera and evaluated their histopathological effects. The seeds were extracted using Soxhlet extraction techniques. The following doses were prepared: 1000, 1600, 2900, and 5000 mg/kg for oral route toxicity testing using Wistar rats. The test group rats received a single oral dose of 1000, 1600, 2900, and 5000 mg/kg body weight of n-hexane oil extracts of both N. sativa and M. oleifera seeds. The control group received an equivalent volume of distilled water. The study showed no mortality in all the rat groups after 24 hours and up to a week after oral
administration of the samples. Changes were noted in the body weights of the rats, with slight dullness at the onset of extracts administration. The histology of the liver sections revealed multiple dose-dependent necrosis, histological lesions, and abnormal sinusoids after two weeks of oral administration of both seed oil extracts. The study concluded that the plant's seed oil extracts are relatively safe for nutritional and medicinal uses.
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