Comparative Antioxidant Activities of Leaf Extracts of Detarium microcarpum Guill & Perr (Fabaceae)
http://www.doi.org/10.26538/tjnpr/v6i11.16
Keywords:
Nitric Oxide, FRAP, DPPH, Antioxidant, Detarium microcarpumAbstract
Detarium microcarpum is a plant that has been used widely to meet the nutritional needs of humans and also to treat various ailments in ethnomedicine. The present study is aimed at comparing the in vitro antioxidant activities of the leaves of D. microcarpum which have been extracted using solvents of varying polarities and also to determine the total phenolic and total flavonoid contents of the various extracts. The leaves of the plant were extracted successively using n-hexane, methanol, ethyl acetate, and water. The extracts' total phenolic and flavonoid contents were determined using a colorimetric assay. The antioxidant activity of the extracts was also determined using the 1, 1-Diphenyl-2-picrylhydrazyl (DPPH), Nitric Oxide, and ferric reducing antioxidant power (FRAP) assay methods. All the extracts were found to have some phenolic and flavonoid content with the ethyl acetate extract having the highest total phenolic and total flavonoid contents (p<0.05). The different extracts demonstrated good antioxidant activities in the DPPH, nitric oxide, and FRAP assay methods.
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