Bio-Activity Investigations of Extracts of Different Parts of Lumnitzera littorea Voigt
doi.org/10.26538/tjnpr/v4i8.8
Keywords:
Antibacterial, Antioxidant, Flavonoid, Phenolic, Lumnitzera littoteaAbstract
The identification of active natural compounds from plants for use as therapeutic agents for
aquatic diseases could reduce the use of environmentally harmful chemicals. Lumnitzera littorea
is a plant in the Combretaceae family that thrives among mangroves. Although well known in
traditional medicine, there are only a few reports of the activity of various parts of this plant
against aquatic pathogenic bacteria. Therefore, this work studied the phytochemical composition
of the stem-bark, leaf, twig, and flower of L. littorea extracts and further investigated the
antioxidant and antibacterial activities. The qualitative and quantitative analyses of phenolic and
flavonoid contents were carried using the Folin-Ciocaltue and the colorimetric aluminium
chloride methods, respectively. Antioxidant activity was determined by 2,2-diphenyl-1-
picrylhydrazyl radical (DPPH) and 2,2/-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)
diammonium salts (ABTS) methods. The extracts were screened for antibacterial activity against
different pathogens (Streptococcus agalactiae, Aeromonas hydrophila, Vibrio harveyi and V.
parahemolyticus) using the hole-plate diffusion method. The results showed the presence of
phenolic and flavonoid components in all extracts. Bark and twig extracts had greater total
phenolic and flavonoid contents than those from flower and leaf extracts. Significant antioxidant
activity was expressed by the bark and twig extracts. All extracts showed a wide range of
antibacterial activity against the tested organisms. Bioactive substances present in L. littorea
extracts exhibited antioxidant and antibacterial activities. L. littorea could be a potential source
of natural bioactive compounds that could be used to develop safe and environmentally friendly
pharmaceuticals against bacterial pathogens in aquatic animals.
Keywords:
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