Rosemarinus officinalis Mediated Synthesis of Silver Nanoparticles and its Activity against Pathogenic Fungal Strains
doi.org/10.26538/tjnpr/v4i6.7
Keywords:
Rosemarinus officinalis, Silver nanoparticles, Candida species,, Antifungal activityAbstract
Nanotechnology is advancing rapidly with applications in various fields. Silver (Ag) nanoparticles are known for their pronounced impact in pharmaceutical industry owing to its strong antimicrobial activity. Antimicrobial resistance of drugs has shifted the focus of the scientific community to the search of novel bioactive compounds from medicinal plants with more potential to treat various ailments. Candida species are widely recognized as pathogens accountable for high morbidity and persistent infection. In this study we elucidated the robustness of biogenic silver nanoparticles synthesized using Rosemarinus officinalis against pathogenic Candida species. We tested and compared the impact of 161 µg/mL, 270 µg/mL concentrations of silver nanoparticles and characterized the particles using UV-VIS Spectrophotometer and Scanning Electron Microscopy (SEM). The antifungal activity of the Silver nanoparticles was tested against Candida albicans, Candida tropicalis, Candida glabrata and Candida krusei using Agar well diffusion method. The synthesized nanoparticles were spherically shaped with size ranging from 75 – 98 nm. The results revealed high efficacy of 161 µg/mL silver nanoparticles against C. albicans, C. tropicalis, and C. krusei compared to 270 µg/mL Ag nanoparticle. Silver nanoparticles can be used as a therapeutic drug instead of synthetic drugs due to its reduced toxicity and adverse effects. Search of naive plants as novel therapeutic agents can open new avenues in drug development to combat antibiotic resistance.
References
Nikalje AP. Nanotechnology and its applications in medicine. Med Chem. 2015; 5(2):081-089.
Singh P, Kim YJ, Zhang D, Yang DC. Biological synthesis of nanoparticles from plants and microorganisms. Trends Biotechnol. 2016; 34(7):588-599.
Rafique M, Sadaf I, Rafique MS, Tahir MB. A review on green synthesis of silver nanoparticles and their applications. Artific Cells Nanomed Biotechnol. 2017; 45(7):1272-91.
Silva LP, Silveira AP, Bonatto CC, Reis IG, Milreu PV. Silver nanoparticles as antimicrobial agents: past, present, and future. In Nanostructures for Antimicrobial Therapy 2017; 577-596.
Abbasi E, Milani M, Fekri Aval S, Kouhi M, Akbarzadeh A, Tayefi Nasrabadi H, Nikasa P, Joo SW, Hanifehpour Y, Nejati-Koshki K, Samiei M. Silver nanoparticles: synthesis methods, bio-applications and properties. Crit Rev Microbiol. 2016; 42(2):173-180.
D’Costa VM, King CE, Kalan L, Morar M, Sung WW, Schwarz C, Froese D, Zazula G, Calmels F, Debruyne R. Antibiotic resistance is ancient. Nature 2011; 477(7365):457.
Akinyemi KO, Bamiro BS, Coker AO. Salmonellosis in Lagos, Nigeria: incidence of Plasmodium falciparum- associated co-infection, patterns of antimicrobial resistance, and emergence of reduced susceptibility to fluoroquinolones. J Health Popul Nutr. 2007; 25(3):351.
Mondal S, Bandyopadhyay SK, Ghosh M, Mukhopadhyay S, Roy S, Mandal C. Natural products: promising resources for cancer drug discovery. Anti-Cancer Agents Med Chem. 2012; 12(1):49-75.
Medda S, Hajra A, Dey U, Bose P, Mondal NK. Biosynthesis of silver nanoparticles from Aloe vera leaf extract and antifungal activity against Rhizopus sp. and Aspergillus sp. Appl Nanosci. 2015; 5(7):875-880.
Jain S and Mehata MS. Medicinal plant leaf extract and pure flavonoid mediated green synthesis of silver nanoparticles and their enhanced antibacterial property. Sci Rep. 2017; 7(1):15867.
Yasir M, Singh J, Tripathi MK, Singh P, Shrivastava R. Green synthesis of silver nanoparticles using leaf extract of common arrowhead houseplant and its anticandidal activity. Pharm Mag. 2017; 13(4):S840.
Ghaedi M, Yousefinejad M, Safarpoor M, Khafri HZ, Purkait MK. Rosmarinus officinalis leaf extract mediated green synthesis of silver nanoparticles and investigation of its antimicrobial properties. J Ind Eng Chem. 2015; 31:167- 172.
Singh A, Jain D, Upadhyay MK, Khandelwal N, Verma HN. Green synthesis of silver nanoparticles using Argemone mexicana leaf extract and evaluation of their antimicrobial activities. Dig J Nanomater Bios. 2010; 5(2):483-489.
Jain KK. Applications of nanobiotechnology in clinical diagnostics. Clin Chem. 2007; 53(11):2002-2009.
Ahmed S, Ahmad M, Swami BL, Ikram S. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J Adv Res. 2016; 7(1):17-28.
Ponarulselvam S, Panneerselvam C, Murugan K, Aarthi N, Kalimuthu K, Thangamani S. Synthesis of silver nanoparticles using leaves of Catharanthus roseus Linn. G. Don and their antiplasmodial activities. Asian Pac J Trop Biomed. 2012; 2(7):574-580.
Gajbhiye M, Kesharwani J, Ingle A, Gade A, Rai M. Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomed Nanotechnology Biol Med. 2009; 5(4):382-386.
Zielińska A, Skwarek E, Zaleska A, Gazda M, Hupka J. Preparation of silver nanoparticles with controlled particle size. Procedia Chem. 2009; 1(2):1560-1566.
Verma A and Mehata MS. Controllable synthesis of silver nanoparticles using Neem leaves and their antimicrobial activity. J Rad Res Appl Sci. 2016; 9(1):109-115.
Panchawat S and Sisodia SS. In vitro antioxidant activity of Saraca asoca Roxb. De Wilde stem bark extracts from various extraction processes. Asian J Pharm Clin Res. 2010; 3(3):231-33 .
Ahmad N, Sharma S. Green synthesis of silver nanoparticles using extracts of Ananas comosus. Green Sust Chem. 2012; 2(04):141.
Shankar SS, Rai A, Ahmad A, Sastry M. Rapid synthesis of Au, Ag, and bimetallic Au core–Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. J Coll Interface Sci. 2004; 275(2):496-502.
Amendola V, Bakr OM, Stellacci F. A study of the surface plasmon resonance of silver nanoparticles by the discrete dipole approximation method: effect of shape, size, structure, and assembly. Plasmonics. 2010; 5(1):85-97.
Pfaller MA, Diekema DJ, Procop GW, Rinaldi MG. Multicenter comparison of the VITEK 2 antifungal susceptibility test with the CLSI broth microdilution reference method for testing amphotericin B, flucytosine, and voriconazole against Candida spp. J Clin Microbiol. 2007;
(11):3522-3528.
Ganguly S and Mitchell AP. Mucosal biofilms of Candida albicans. Curr Opin Microbiol. 2011; 14(4):380-385.
Shrivastava S, Bera T, Roy A, Singh G, Ramachandrarao P, Dash D. Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnol. 2007; 18(22):225103.
Vila T, Romo JA, Pierce CG, McHardy SF, Saville SP, Lopez-Ribot JL. Targeting Candida albicans filamentation for antifungal drug development. Virulence. 2017; 8(2):150-158.
Agha MA, Agha SA, Sharafat S, Zafar MN, Khanani MR, Mirza MA. Candida Glabrata: An emerging threat for the Immunocompromised. Gomal J Med Sci. 2011; 9:115-119.
Nabikhan A, Kandasamy K, Raj A, Alikunhi NM. Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from saltmarsh plant, Sesuvium portulacastrum L. Coll Surf. B. 2010; 79(2):488-493.
Panáček A, Kvitek L, Prucek R, Kolář M, Večeřová R, Pizúrová N, Sharma VK, Nevěčná TJ, Zbořil R. Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. J Phy Chem B. 2006; 110(33):16248- 16253.
Sondi I and Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Coll Inter Sci. 2004; 275(1):177- 182.
Dehkordi SH, Hosseinpour F, Kahrizangi AE. An in vitro evaluation of antibacterial effect of silver nanoparticles on Staphylococcus aureus isolated from bovine subclinical mastitis. Afr J Biotechnol. 2011; 10(52):10795-10797.
Kim KJ, Sung WS, Suh BK, Moon SK, Choi JS, Kim JG, Lee DG. Antifungal activity and mode of action of silver nano-particles on Candida albicans. Biometals. 2009; 22(2):235-242.
Alvarez-Peral FJ, Zaragoza O, Pedreno Y, Argüelles JC. Protective role of trehalose during severe oxidative stress caused by hydrogen peroxide and the adaptive oxidative stress response in Candida albicans. Microbiol. 2002; 148(8):2599-2606.
Scordino F, Giuffrè L, Barberi G, Marino Merlo F, Orlando MG, Giosa D, Romeo O. Multilocus sequence typing reveals a new cluster of closely related Candida tropicalis genotypes in Italian patients with neurological disorders. Front Microbiol. 2018; 9:679.
Thakkar KN, Mhatre SS, Parikh RY. Biological synthesis of metallic nanoparticles. Nanomed Nanotechnology Biol Med. 2010; 6(2):257-262.
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