An Investigation of the Effect of Seasonal Variation on the Phytochemical Constituents in Two Nauclea Species
doi.org/10.26538/tjnpr/v4i4.9
Keywords:
Nauclea diderrichii, Nauclea latifolia, Secondary metabolites, Infrared Spectroscopy, Thin layer chromatographyAbstract
Nauclea diderrichii and Nauclea latifolia are two important African medicinal plants with several known bioactive phytochemicals. However, the presence of these phytochemicals can vary depending on the season of collection. Thus, this study investigates N. diderrichii and N. latifolia leaf samples collected in October (rainy season) and February (dry season) for any disparity in their phytochemical constituents. Pulverized samples were macerated for 24 hours in acetone. The dried extracts were subjected to phytochemical screening following standard procedures. Their Fourier transform infrared (FTIR) spectra were acquired and compared. Thin layer chromatographic analysis were carried out using the chloroform soluble fraction (CSF) and methanol soluble fraction (MSF)] of the extracts on pre-coated silica gel plates. The number of separated spots in each plate was noted after the developed plates were visualized under UV light and after spraying with vanillin- sulphuric acid reagent. Nauclea latifolia leaf extracts showed some variations in saponin and tannin contents as they were higher in rainy season, the number of fundamental IR vibrational peaks were more in rainy season, and the number of spots on TLC for the CSF and MSF varied. For N. diderrichii, in both seasons, no variation was observed. The study revealed some disparities in phytochemical constituents in N. latifolia leaf due to seasonal variation, while this was not so apparent with N. diderrichii leaf.
References
Haudecoeur R, Peuchmaur M, Pérès B, Rome M, Taïwe GS, Boumendjel A, Boucherle B. Traditional uses, phytochemistry and pharmacological properties of African Nauclea species: A review. J Ethnopharmacol. 2018; 212:106–136.
Isa H, Katsayal UA, Agunu A, Nuhu A, Abdulhamid Z. Phytochemical screening and thin layer chromatographic profile of Nauclea diderrichii leaf extracts. Bayero J Pure Appl Sci. 2017; 10(1):281–284.
Abbah J, Amos S, Chindo B, Ngazal I, Vongtau HO, Adzu B, Farida T, Odutola AA, Wambebe C, Gamaniel KS. Pharmacological evidence favouring the use of Nauclea latifolia in malaria ethnopharmacy: Effects against nociception, inflammation, and pyrexia in rats and mice. J Ethnopharmacol. 2010; 127(1):85–90.
Boucherle B, Haudecoeur R, Queiroz EF, De Waard M, Wolfender J-L, Robins RJ, Boumendjel A. Nauclea latifolia: Biological activity and alkaloid phytochemistry of a West African tree. Nat Prod Rep. 2016; 33(9):1034–1043.
Ademola IO, Fagbemi BO, Idowu SO. Anthelmintic efficacy of Nauclea latifolia extract against gastrointestinal nematodes of sheep: In vitro and in vivo studies. African J Trad Compl Altern Med. 2007; 4(2):148–156.
Di Giorgio C, Lamidi M, Delmas F, Balansard G, Ollivier E. Antileishmanial activity of quinovic acid glycosides and cadambine acid isolated from Nauclea diderrichii. Planta Med. 2006; 72(15):1396–1402.
Lamidi M, Ollivier E, Gasquet M, Faure R, Nze-Ekekang L, Balansard G. Structural and antimalarial studies of saponins from Nauclea diderrichii bark. In: Waller, Yamasaki (Editors). Saponins used in traditional and modern medicine. New York: Plenum Press, 1996. 383-399 p.
Ogbole OO, Aliu LO, Abiodun OO, Ajaiyeoba EO. Alpha- amylase inhibition and brine shrimp lethality activities of nine medicinal plant extracts from South-West Nigerian ethnomedicine. J Herbs Spices Med Plants. 2016; 22(4):319–326.
Ncube B, Finnie JF, Staden J Van. Seasonal variation in antimicrobial and phytochemical properties of frequently used medicinal bulbous plants from South Africa. South Afr J Bot. 2011; 77:387–396.
Abreu IN De and Mazzafera P. Effect of water and temperature stress on the content of active constituents of Hypericum brasiliense Choisy. Plant Physiol Biochem. 2005; 43:241–248.
Sofowora A. Medicinal plants and traditional medicine in Africa. 2nd ed. Ibadan: Spectrum Books; 1993. 261-263 p.
Evans WC. Trease and Evans Pharmacognosy. 13th ed. London: Bailliere Tindall; 1989. 378-546 p.
Watson DG. Pharmaceutical analysis - A textbook for pharmacy students and pharmaceutical chemists. 1st ed. London: Churchill Livingstone; 1999. 282 p.
British Pharmacopoeia Commission. British Pharmacopoeia.7th ed. London: The Stationery Office; 2013, Volume 5 (Appendices). 19 p.
Daniel LR, Scott RG, David WB. Chemistry: principles and practice. 3rd ed. Belmont: Brooks/Cole; 2010; 957 p
Eloff JN. Which extractant should be used for the screening and isolation of antimicrobial components from plants? J Ethnopharmacol. 1998; 60(1):1–8.
Eloff JN, Angeh IE, Mcgaw LJ. Solvent-solvent fractionation can increase the antifungal activity of a Melianthus comosus (Melianthaceae) acetone leaf extract to yield a potentially useful commercial antifungal product. Ind Crops Prod. 2017; 110:103–112.
Candeias MF, Abreu P, Pereira A, Cruz-morais J. Effects of strictosamide on mouse brain and kidney Na+, K+-ATPase and Mg2+-ATPase activities. J Ethnopharmacol. 2009; 121:117–122.
Erdelmeier CAJ, Wright AD, Baumgartner B, Rali T. New indole alkaloid glycosides from Nauclea orientalis. Planta Med. 1991; 57:149–152.
Lamidi M, Ollivier E, Faure R, Debrauwer L, Nze-Ekekang L, Balansard G. Quinovic acid glycosides from Nauclea diderrichii. Phytochem. 1995; 38:209–212.
Sun JY, Lou HX, Xu H, Dai SJ, Liu K. Two new indole alkaloids from Nauclea officinalis. Chin Chem Lett. 2007; 18:1084–1086.
Jeffery GH, Bassett J, Mendham J, Denney RC. Vogel's textbook of quantitative chemical analysis. 5th ed. England: Longman House; 1989; 744 p.
Mayo DW, Miller FA, Hannah RW. Course notes on the interpretation of infrared and raman spectra. New Jersey: John Wiley & Sons Inc., Hoboken; 2004. 1-462 p.
Ramírez-Briones E, Rodríguez-Macías R, Salcedo-Pérez E, Ramírez-Chávez E, Molina-Torres J, Tiessen A, Ordaz- Ortiz J, Martínez-Gallardo N, Délano-Frier JP, Zañudo- Hernández J. Seasonal changes in the metabolic profiles and biological activity in leaves of Diospyros digyna and D. rekoi “Zapote” trees. Plants 2019; 8(449):1-22.
Peters K, Gorzolka K, Bruelheide H, Neumann S. Seasonal variation of secondary metabolites in nine different bryophytes. Ecol Evol. 2018; 8:9105–9117.
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