Alpha-Amylase Inhibitory Activity of Extract Combination of Morinda citrifolia L. Fruit, Trigonella foenum-graecum and Nigella sativa L. Seeds Using In vitro and In Vivo Assay
doi.org/10.26538/tjnpr/v4i10.23
Keywords:
Trigonella foenum-graecum L., Nigella sativa L., Morinda citrifolia L., IC50, Diabetes mellitus, Blood glucose levelAbstract
The poly-herbal formulation is being evaluated for effective diabetes management. However, only a few formulations have made significant inroads as treatment alternatives. This research was carried out to determine the in vitro and in vivo alpha-amylase inhibitory activity of extract combination of Morinda citrifolia L fruits, Trigonella foenum-graecum and Nigella sativa L. seeds, because each extract was known to have antidiabetic properties. In vitro alpha amylase activity was carried out using dinitrosalicylic acid (DNS) assay while the in vivo alpha amylase activity was performed using experimental rat model. Twenty-four mice (Mus musculus L.) were
randomly divided into six groups. Group 1 served as the control and was given distilled water only, while group II was induced with alloxan (40 mg/kg i.p.) only. Groups III, IV, V and VI rats were induced with alloxan, then after 48 h of induction, the rats were given acarbose, dose 1 (70:70:280 mg/kg BW): dose 2 (140:70:140 mg/kg BW) and dose 3 (70:140:140 mg/kg BW), respectively. The three combination of extracts showed inhibitory alpha-amylase activity, with combination of dose 2 as the best potential inhibition. The combination of dose 2 has an IC50 value of 35.70 ± 1.33 µg/mL and the percentage reduction in the average blood glucose levels at
the 30th, 60th, 120th minute were shown to be 11.98%, 25.85%, and 34.80%, respectively.
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