Isolation of Isosativan from Nigerian Red Propolis

doi.org/10.26538/tjnpr/v4i3.3

Authors

  • Bawazeer Sami College of Pharmacy, Umm Al-qura University, Makkah, Saudi Arabia.
  • Henry Okoro Department of Chemistry, University of Agriculture, Makurdi Nigeria
  • Ngozichukwuka P. Igoli Centre for Food Technology and Research, Benue State University Makurdi Nigeria.
  • John O. Igoli2 Department of Chemistry, University of Agriculture, Makurdi Nigeria.

Keywords:

Isoflavan,, β-amyrin, Propyl stearate, Oleic acid,, Rivers State

Abstract

Propolis has been known for its medicinal use and as a source of diverse bioactive natural compounds. Phytochemical screening of propolis samples usually reveal the constituent compounds and the source of the sample. Chromatography is the simplest way of isolating natural compounds and thus the hexane and ethyl acetate extracts of propolis from Bonny in Rivers State, Nigeria were subjected to column chromatography and the isolated compounds were identified by LC-MS and NMR analysis. The compounds detected and identified were oleic acid, propyl stearate, β-amyrin, isosativan, calycosin, liquiritigenin, isoliquiritigenin, pinocembrin and medicarpin. The structure of Isosativan was further confirmed by HRMS and 2D NMR spectroscopy. This is the first report of Isosativan from a sample of Nigerian and African propolis.

 

References

Burdock G. Review of the biological properties and toxicity of bee propolis. Food Chem Toxicol. 1998; 36:347–352.

Marcucci, MC. Propolis: Chemical composition, biological properties and therapeutic activity. Apidologie 1995; 26: 88-90.

Blicharska N and Seidel V. Chemical Diversity and Biological Activity of African Propolis in A. D. Kinghorn, H. Falk, S. Gibbons, J. Kobayashi, Y. Asakawa, J.-K. Liu (eds.), Prog Chem Org Nat Prod. 2019; 109:415-450.

Huang S, Zhang C, Wang K, Li GQ, Hu F Recent Advances in the Chemical Composition of Propolis. Molecules 2014; 19:19610-19632.

Bankova, V. New biologically active compounds from Kenyan propolis. Fitoterapia 2010; 81:509–514.

Alaribe CS, Esposito T, Sansone F, Adesegun S, Pagano I, Piccinelli AL, Celano R, Rubio OC, Coker HA, Nabavi SM, Rastrelli L, Patrizia Picerno P. Nigerian propolis: chemical composition, antioxidant activity and α-amylase and α- glucosidase inhibition, Nat Prod Res. 2019; 8:1-5.

Omar R, Igoli JO, Zhang T, Gray AI, Ebiloma GU, Clements CJ, Fearnley J, Edrada-Ebel R, Paget T, de Koning HP, Watson DG. The chemical characterization of Nigerian propolis samples and their activity against Trypanosoma brucei. Sci Rep. 2017; 7(923):1-10.

Omar RMK, Igoli J, Gray AI, Ebiloma GU, Clements C, Fearnley J, Edrada-Ebel R, Zhang T, Harry P. De Koning, David G. Watson. Chemical characterisation of Nigerian red propolis and its biological activity against Trypanosoma brucei. Phytochem Anal. 2015; 27:107-115.

Nweze NE, Okoro HO, Al Robaian M, Omar RMK, Tor- Anyiin TA, Watson DG Igoli JO. Effects of Nigerian red propolis in rats infected with Trypanosoma brucei brucei. Comp Clin Pathol. 2017; 26:1129-1133.

Panche, AN, Diwan AD, Chandra SR. Flavonoids: an overview. J Nutr Sci. 2016; 5(e47):1-15.

Silva BB, Rosalen PL, Cury JA, Ikegaki M, Souza VC, Esteves A, Alencar SM. Chemical composition and botanical origin of red propolis, a new type of Brazilian propolis. Evidence-based Complementary and Alternative Medicine: eCAM, 2008; 5(3):313–316.

Trusheva B, Popova M, Bankova V, Simova S, Marcucci MC, Miorin PL, da Rocha Pasin F, Tsvetkova I. Bioactive constituents of Brazilian red propolis. Evid Based Compl Altern Med. 2006; 3(2):249-254.

Dias MM, Hamerski L, Pinto A. Separacao semipreparative de α e β-amyrina por cromatografia líquida de alta eficiencia, Qumica Nova, 2011; 34(4):704-706.

Brito GAB and Chaves MH. β-amyrin, a natural triterpenoid ameliorates L-arginne-induced acute pancreatitis in rats. World J Gastroenterol. 2010; 16(34):4272-4280.

Yamaji T, Saito T, Hyamizu K, Yanayisawa M, Ymamoto O. (2014). Spectra Database for organic compound (SDBS): methyl stearate and oleic acid. National institute for advanced Industrial Science and technology. [Online]. 2014 [cited 2019 Dec 30] Available from: https://sdbs.db.aist.go.jp/sdbs/cgi-bin/direct_frame_top.cgi

Urangaa CC, Beld J, Mrse A, Córdova-Guerreroc I, Burkart MD, Hernández-Martínez, R. Data from mass spectrometry, NMR spectra, GC–MS of fatty acid esters produced by Lasiodiplodia theobromae Data in Brief 2016; 8: 31–39.

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Published

2020-03-01

How to Cite

Sami, B., Okoro, H., P. Igoli, N., & O. Igoli2, J. (2020). Isolation of Isosativan from Nigerian Red Propolis: doi.org/10.26538/tjnpr/v4i3.3. Tropical Journal of Natural Product Research (TJNPR), 4(3), 77–79. Retrieved from https://www.tjnpr.org/index.php/home/article/view/1041

Issue

Vol. 4 No. 3 (2020): Tropical Journal of Natural Product Research

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Articles

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