Artemisia judaica Attenuates Hyperglycaemia-Mediated Oxidative Stress and Cardiac Injury in Streptozotocin-Induced Diabetic Rats doi.org/10.26538/tjnpr/v4i10.11

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Wesam al-Amarat

Abstract

Hyperglycemia-induced oxidative stress is a recognized risk factor for cardiovascular diseases and heart failure. The study investigated the cardioprotective effect of Artemisia judaica (A. judaica) on diabetes-induced oxidative injury in rats. A rat model of diabetes was achieved by intraperitoneal (i.p.) injection of streptozotocin (55 mg/kg). After confirmation of diabetes, rats were treated with A. judaica (300 mg kg−1 day−1, p.o.) daily for six weeks. Diabetic rats demonstrated a significant increase in fasting blood glucose and glycosylated hemoglobin (HbA1c) and decrease in insulin levels. Hyperglycaemia-induced cardiac injury was characterised by increased levels of creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) activities in the plasma, along with several histological alterations in the myocardium. The hearts of diabetic rats showed increased levels of malondialdehyde (MDA), with a significant decrease in the content of glutathione (GSH) and the activities of superoxide dismutase (SOD) and catalase (CAT). The oral A. judaica treatment ameliorated hyperglycaemia, prevented hyperglycaemia-induced cardiac injury, boosted antioxidants and suppressed oxidative stress. These findings showed that A. judaica protected against diabetes-induced cardiac injury through attenuation of oxidative stress. However, the exact mechanism underlying the cardioprotective effects of A. judaica undoubtedly deserves further exploration in future studies.

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How to Cite
al-Amarat, W. (2020). Artemisia judaica Attenuates Hyperglycaemia-Mediated Oxidative Stress and Cardiac Injury in Streptozotocin-Induced Diabetic Rats: doi.org/10.26538/tjnpr/v4i10.11. Tropical Journal of Natural Product Research (TJNPR), 4(10), 722–727. Retrieved from https://www.tjnpr.org/index.php/home/article/view/1040
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References

Varga ZV, Giricz Z, Liaudet L, Haskó G, Ferdinandy P, Pacher P. Interplay of oxidative, nitrosative/nitrative stress, inflammation, cell death and autophagy in diabetic cardiomyopathy. Biochim Biophys Acta. 2015;1852(2):232- 242.

Al Hroob AM, Abukhalil MH, Alghonmeen RD, Mahmoud AM. Ginger alleviates hyperglycemia-induced oxidative stress, inflammation and apoptosis and protects rats against diabetic nephropathy. Biomed Pharmacother. 2018;106:381- 389.

Althunibat OY, Al Hroob AM, Abukhalil MH, Germoush MO, Bin-Jumah M, Mahmoud AM. Fisetin ameliorates oxidative stress, inflammation and apoptosis in diabetic cardiomyopathy.Life Sci. 2019;221:83-92.

Othman AI, El-Sawi MR, El-Missiry MA, Abukhalil MH. Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats. Biomed Pharmacother. 2017;94:362-373.

Amin AH, El-Missiry MA, Othman AI. Melatonin ameliorates metabolic risk factors, modulates apoptotic proteins, and protects the rat heart against diabetes-induced apoptosis. Eur J Pharmacol. 2015;747:166-173.

Atrooz OM, Abukhalil MH, AlRawashdeh IM. Characterization of β-galactosidase in the Crude Plant Extract of Artemisia judaica L. in Presence and Absence of Some Heavy Metals.Am J Life Sci. 2016;4(5):99-105.

Abu-Darwish MS, Cabral C, Gonçalves MJ, Cavaleiro C, Cruz MT, Zulfiqar A, Khan IA, Efferth T, Salgueiro L. Chemical composition and biological activities of Artemisia judaica essential oil from southern desert of Jordan. J Ethnopharmacol. 2016;191:161-168.

Albasher G, Aljarba N, Al Sultan N, Alqahtani WS, Alkahtani S. Evaluation of the neuro‐protective effect of Artemisia judaica extract in a murine diabetic model. Food Biochem. 2020;44(8):e13337.

Mesa LE, Lutgen P, Velez ID, Segura AM, Robledo SM. Artemisia annua L., potential source of molecules with pharmacological activity in human diseases.AJPCT. 2015;3(5):436-450.

Abdalla SS and Zarga MA. Effects of cirsimarit in, a flavone isolated from Artemisia judaica, on isolated guinea-pig ileum.Planta Med. 1987; 53(04):322-324.

Benmansour N, Benmansour A, El Hanbali F, González‐Mas MC, Blázquez MA, El Hakmaoui A, Akssira M. Antimicrobial activity of essential oil of Artemisia judaica L. from Algeria against multi-drug resistant bacteria from clinical origin. Flavour Fragr J. 2016; 31(2):137-142.

Acheuk F, Lakhdari W, Abdellaoui K, Belaid M, Allouane R, Halouane F. Phytochemical study and bioinsecticidal effect of the crude ethonolic extract of the Algerian plant Artemisia judaica L.(Asteraceae) against the black bean aphid, Aphis fabae Scop. Poljoprivreda i Sumarstvo.

;63(1):95.

Abdelgaleil SA, Abbassy MA, Belal AS, Rasoul MA. Bioactivity of two major constituents isolated from the essential oil of Artemisia judaica L.Bioresour Technol. 2008;99(13):5947-5950.

Mahboubi M and Kazempour N. Biochemical activities of Iranian Cymbopogonolivieri (Boiss) Bor. essential oil.Indian J Pharm Sci. 2012;74(4):356.

Sitzmann J, Habegger R, Schnitzler WH, Grassmann J. Comparative analysis of antioxidant activities of fourteen Mentha essential oils and their components. Chem. Biodivers. 2014;11(12):1978-1989.

Liu CZ, Murch SJ, El-Demerdash M, Saxena PK. Artemisia judaica L.: micropropagation and antioxidant activity. J. Biotechnol. 2004;110(1):63-71.

El-Sayed MA, BaAbbad R, Balash A, Al-Hemdan NA, Softah A. The potential anti Helicobacter pylori and antioxidant effects of Artemisia judaica.Funct Food Health Dis. 2013;3(9):332-340.

Nofal SM, Mahmoud SS, Ramadan A, Soliman GA, Fawzy R. Anti-diabetic effect of Artemisia judaica extracts.Res J Med Sci. 2009;4(1):42-48.

Ahmed ES, Mabrouk DM, Hassanane MM, Khalil WK. Protective effect of Artemisia judaica against doxorubicininduced toxicity in mice. Annu Res Rev. 2017:1-10.

Althunibat OY, Al-Mustafa AH, Tarawneh K, Khleifat KM, Ridzwan BH, Qaralleh HN. Protective role of Punica granatum L. peel extract against oxidative damage in experimental diabetic rats. Proc Biochem. 2010;45(4):581- 585.

Abd-Alla HI, Aly HF, Shalaby NM, Albalawy MA, Aboutabl EA. Hunting for renal protective phytoconstituents in Artemisia judaica L. and Chrysanthemum coronarium L.(Asteraceae). Egy Pharm J. 2014;13(1):46.

Mamoru S and Kazuyuki H. A new colorimetric method for determination of serum glucose. Clin Chim Acta. 1977;75(3):387-391.

Bae J, Min YS, Nam Y, Lee HS, Sohn UD. Humulus japonicus extracts protect against lipopolysaccharide/dgalactosamine-induced acute liver injury in rats. J Med Food. 2018;21(10):1009-1015.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem.. 1979;95(2):351-358.

Beutler E. Improved method for the determination of blood glutathione. J Lab Clin Med.1963;61:882-888.

Nishikimi M, Rao NA, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen.Biochem Biophys Res Commun. 1972;46(2):849-854.

Aebi H. Catalase in vitro. In Meth Enzymol. 1984; 1(105): 121-126.

Classics Lowry O, Rosebrough N, Farr A, Randall R. Protein measurement with the Folin phenol reagent. JBiol Chem. 1951;193:265-275.

Farshid AA, Tamaddonfard E, Moradi-Arzeloo M, Mirzakhani N. The effects of crocin, insulin and their coadministration on the heart function and pathology in streptozotocin-induced diabetic rats. Avicenna J Phytomed. 2016;6(6):658.

Al Hroob AM, Abukhalil MH, Alghonmeen RD, Mahmoud AM. Ginger alleviates hyperglycemia-induced oxidative stress, inflammation and apoptosis and protects rats against diabetic nephropathy. Biomed Pharmacother. 2018;106:381- 389.

Al-Rasheed NM, Al-Rasheed NM, Hasan IH, Al-Amin MA, Al-Ajmi HN, Mohamad RA, Mahmoud AM. Simvastatin ameliorates diabetic cardiomyopathy by attenuating oxidative stress and inflammation in rats. Oxid Med Cell Longev. 2017; 2017:1-13.

Gupta SK, Dongare S, Mathur R, Mohanty IR, Srivastava S, Mathur S, Nag TC. Genistein ameliorates cardiac inflammation and oxidative stress in streptozotocin-induced diabetic cardiomyopathy in rats. Mol Cell Biochem. 2015;408(1-2):63-72.

Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001;50(6):537-546.

Lenzen S, Drinkgern J, Tiedge M. Low antioxidant enzyme gene expression in pancreatic islets compared with various other mouse tissues.Free Radic. Biol. Med. 1996;20(3):463- 466

Albasher G, Alwahaibi M, Abdel-Daim MM, Alkahtani S, Almeer R. Protective effects of Artemisia judaica extract compared to metformin against hepatorenal injury in high-fat diet/streptozotocine-induced diabetic rats. Environ Sci PollutRes. 2020; 7:40525–40536.

Bhat SH, Ullah MF, Abu-Duhier FMJOP, Medicine E. Bioactive extract of Artemisia judaica causes in vitro inhibition of dipeptidyl peptidase IV and pancreatic/intestinal enzymes of the carbohydrate absorption cascade: Implication for anti-diabetic new molecular entities (NMEs). Orient

Pharm Exp Med. 2019; 19(1):71-80.

Wilcox G. Insulin and insulin resistance. Clin Biochem Rev. 2005;26(2):19.

Bodor GS. Biochemical markers of myocardial damage. EJIFCC. 2016;27(2):95.

Muthumani M and Miltonprabu S. Arsenic-induced oxidative stress and its possible reversal by chelation therapy. Res Rev J Toxicol. 2012;2:16-37.