Antifungal activity of Ethyl-acetate Leaf Extract and Terpenoid-Rich Fraction from Hyptis spicigera Lam
doi.org/10.26538/tjnpr/v4i3.1
Keywords:
Antifungal,, Phytochemical,, Ethyl-acetate,, Terpenoids-rich fractionAbstract
Plant extracts act as contact fungicides by disrupting cell membrane integrity at different stages of fungal development, while others inactivate key enzymes. The present study is aimed at evaluating the phytochemical constituents and antifungal activity of the ethyl-acetate extract and terpenoid-rich fraction from Hyptis spicigera. Qualitative phytochemical analysis was done using standard methods and quantitative analysis was done spectrophotometrically. Antifungal activity was done using Agar well diffusion method. Qualitative phytochemical analysis revealed the presence of anthraquinones, sterols, triterpenes, cardiac glycosides, flavonoids, tannins and alkaloids. The quantitative phytochemical screening revealed high concentrations of saponins (920 mg/g) and phenolics (200 mg/g). Sensitivity test of the extract and terpenoid-rich fraction on the test organisms revealed highest diameter zone of inhibition against Aspergillus parasiticus (24.67 ± 0.88 mm) and the least inhibition zone against Fusarium oxysporum (18.00± 0.58 mm). Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the ethyl-acetate extract was observed at concentrations ranging between 6.25 and 12.5 mg/mL. The MIC of the terpenoid-rich fraction was observed at concentrations ranging between 6.25 mg/mL and 12.5 mg/mL while the MFC were between 12.5 mg/mL to 25 mg/mL. The result of the antifungal activity revealed that there was a significant difference between the ethyl-acetate extract, terpenoid-rich fraction and the control fungicide (mancozeb). The result obtained from this research suggest that the leaf extract of Hyptis spicigera and its terpenoid-rich fraction could be used as bio-fungicide for the control of fungal diseases caused by species of the genus Aspergillus and Fusarium.
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