Expanding the Frontiers of Bacterial Diagnosis through Bacteriophage Biotechnology

Tolulope J. Oduselu1*, Oluwafolajimi A. Adesanya2, Oluwasegun I. Daramola1, Ayobami J. Akomolafe1, Olubusuyi M. Adewumi3
1Department of Biomedical Laboratory Science, College of Medicine, University of Ibadan, Ibadan, Nigeria
2Department of Medicine, College of Medicine, University of Ibadan, Ibadan, Nigeria
3Department of Virology, College of Medicine, University of Ibadan, Ibadan, Nigeria

Corresponding Author: [email protected]; Tel: +234-09097764112
Recieved Date: August 28, 2020; Accepted Date: November 24, 2020; Published Date: 30 November 2020
Citation: Oduselu TJ, Adesanya OA, Daramola OI, Akomolafe AJ, Adewumi OM. Expanding the Frontiers of Bacterial Diagnosis through Bacteriophage Biotechnology. Trop J Nat Prod Res. 2020; 4(11):855-860.  https://doi.org/10.26538/tjnpr/v4i11.2
Copyright: © 2020 Oduselu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

In a bid to achieve microbial diagnostic precision and reduce diagnostic turn-around time, the development of technologically advanced novel techniques has been on the rise. There is a gradual phasing out of traditional diagnostic methods by more specific and highly sensitive molecular techniques. Asides from being technically demanding and cost-ineffective, these molecular methods have themselves not fulfilled perhaps the most essential diagnostic criterion of distinguishing between viable and dead bacterial cells. The use of bacteriophages as biorecognition elements for bacterial detectors offers numerous advantages in terms of cost, ease of accessibility, and high specificity binding of bacteriophages to their bacterial host. Biotechnological advancements further give bacteriophage use the leading edge as genetic modification of bacteriophage genome through the fluorescent gene insertion produces reporter bacteriophages. These recombinants can produce detectable fluorescent signals through intracellular lytic action, strictly in metabolically active bacteria cells. Fluorescent labelled enzyme-active and cell wall binding domains of bacteriophages also offer better alternatives to the use of antibodies as diagnostic markers because they are resistant to pH and temperature sensitivities. Overall, bacteriophage-based detection systems are less prone to detection errors and significantly reduce diagnostic time while also attaining high test sensitivity.

Keywords: Reporter bacteriophage, Fluorescent protein, Biotechnology, Phage-based sensors.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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