Fruit Wastes as Substrate For the Production of Amylase by Aspergillus niger

Cyprian E. Oshoma1*, Henrietta O. Obueh2, Sophia O. Eguakun-Owie1, Solomon E. Omonigho1
1Department of Microbiology, Faculty of Life Sciences, University of Benin, P.M.B. 1154 Nigeria.
2Biology Department, College of Education, Ekiadolor, P.M.B. 1144, Benin City, Nigeria
Corresponding Author: [email protected]; Tel: +2349099727664
Recieved Date: September 18, 2017; Accepted Date: October 04, 2017; Published Date: 05 October 2017
Citation: Oshoma CE, Obueh HO, Eguakun-Owie SO, Omonigho SE. Fruit Wastes as Substrate for the Production of Amylase by Aspergillus niger. Tropical Journal of Natural Product Research 2017; 1(4):182-185. doi.org/10.26538/tjnpr/v1i4.9 https://doi.org//10.26538/tjnpr/v1i4.9
Copyright: © 2017 Oshoma et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Waste generation demands that measures must be put in place in order to avert their detrimental effect to the environment. Bioconversion of agricultural waste to useful products like enzyme is a welcome development. Amylase production by Aspergillus niger via submerged fermentation of fruit wastes such as pineapple, orange, banana, cucumber and watermelon was investigated. Biomass of A. niger, amylase produced and pH of the fermenting fruit waste media were determined using standard techniques during submerged fermentation. From the data obtained, Banana waste medium produced highest yield of A. niger amylase (259.00 ± 1.23 U/mL) compared to other waste media at 4 d of fermentation. Amylase yield from Banana waste medium was significantly higher compared with the other fruit wastes (p < 0.05). Among the various supplemented nitrogen sources in the Banana waste medium, ammonium nitrate gave the highest amylase yield of 881.00 ± 16.97 U/mL while the least (549.5 ± 20.51 U/mL) was from potassium nitrate medium. Thus, the study revealed that A. niger can be used for amylase production from fruit waste and optimal yield can be enhanced by supplementing the medium with ammonium nitrate.

Keywords: Amylase, Banana waste, Aspergillus niger, Submerged fermentation, Optimization
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