The Use of ISSR Marker for Analyzing the Genetic Diversity of Nigerian Date Palms (Phoenix dactylifera L.)

Beatrice O. Emoghene1*, Chukwuemeka R. Eke1, Omorefe Asemota1, McDonald Idu2
1Physiology Division, Nigerian Institute for Oil palm Research (NIFOR), PMB 1030, Benin City, Nigeria.
2Department Plant Biology and Biotechnology University of Benin, Benin City, Nigeria.
Corresponding Author: [email protected]; Tel: +234-80 23555479
Recieved Date: August 21, 2017; Accepted Date: September 09, 2017; Published Date: 09 September 2017
Citation: Emoghene BO, Eke CR, Asemota O, Idu M. The Use of ISSR Marker for Analyzing the Genetic Diversity of Nigerian Date Palms (Phoenix dactylifera L.). Tropical Journal of Natural Product Research 2017; 1(3):133-137. https://doi.org/10.26538/tjnpr/v1i3.10
Copyright: © 2017 Emoghene et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Date palm (Phoenix dactylifera L.) is a major fruit crop of arid climate region in countries of the Middle East and North Africa. DNA markers are powerful tools that can provide information on the relatedness among date palms that are difficult to distinguish morphologically. The present study was aimed at the analysis of genetic diversity that exists among and within 10 date palms ((RCP1 (tissue culture palm), Medjoole, R4P2, R5P7, R6P8, R7P6, R8P1, R8P4, R8P6 and R8P13) grown at the Nigerian Institute for Oil Palm Research (NIFOR) experimental Substation Dutse, Jigawa State using Inter-Simple Sequence Repeat (ISSR) markers (14A, 44B, HB-08, HB-10, HB-12 and HB-14). DNA was extracted from the 10 date palm leaf samples using DNeasy Mini Kit, PCR amplification was done using ISSR markers and amplification products were analyzed by electrophoresis in 2 % agarose gel stained with SYBR Green and photographed under UV light. Among the six ISSR markers used to cluster the 10 date palms, 44B, HB-10 and HB-12 gave the highest variation percentage polymorphism. Genetic distance and relationship were estimated among the date palm varieties. The highest similarity coefficient value (0.84) was observed between R5P7 and R8P6 which seems to be the nearest two varieties and can be closely regrouped. The similarity coefficient value of 0.00 was obtained between RCP1 and Medjoole date palms, indicating that these were among themselves similar but distinct from the other date palms. The result from this work showed evidence of divergence among the date palms grown in Nigeria.

Keywords: Genetic, diversity, ISSR, marker, Polymorphism, Date palm, DNA
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