In vitro Diosgenin Augmentation in Microtubers of Dioscorea floribunda (M. Martens & Galeotti)

Joy I. Odimegwu1, 2, Ritesh K. Yadav1, Steve O. Ogbonnia2, Olukemi A. Odukoya2, Neelam S. Sangwan1*
1CSIR-Central Institute for Medicinal and Aromatic Plants, CIMAP. Lucknow, India.
2Department of Pharmacognosy, Faculty of Pharmacy, College of Medicine Campus, University of Lagos, Lagos, Nigeria.
Corresponding Author's Email: [email protected]
ABSTRACT

Medicinal yam Dioscorea floribunda is known to possess pharmacologically active diosgenin, a steroidal sapogenin, usually found in greater quantities in the tubers. It is used for the commercial synthesis of cortisone, pregnenolone, progesterone, and other steroid products. Diosgenin is also implicated in reduction of serum cholesterol, estrogenic activity and anticancer effects. In the present study, multiple shoots were initiated from nodal explants and microtuberization, from budding nodal segments of D. floribunda using Murashige and Skoog medium supplemented with varying quantities and combinations of plant hormones; benzyl amino purine, indole-3-butyric acid, naphthalene acetic acid and kinetin. There was a differential response in the medium consisting of hormones and their concentrations on diosgenin accumulation in tubers. The best performance was observed in media supplemented with 2 mg L?1 naphthalene acetic acid and 0.5 mg L?1 kinetin producing viable tubers only in 3 months of culture period and accumulating the higher amount of diosgenin content. Also, results reveal that the faster multiple shoot formation and microtuberization is achieved more efficiently in liquid media. Thus, we have achieved diosgenin production and augmentation in microtubers of D. floribunda.

Keywords: Dioscorea floribunda, Diosgenin, Naphthalene acetic acid, Microtuberization
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