Alpha Amylase Inhibitory and Antioxidant Activities of Leaf Extract and Fractions of Sorindea warneckei Engl. (Anacardiaceae)

Sunday. A. Adesegun1*, Motunrayo V. Badejo1, Samuel O. Odebunmi1, Peter D. Ojobo2, Herbert B. Coker3
1Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Nigeria.
2Central Research Laboratory, College of Medicine, University of Lagos, Nigeria.
3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Lagos, Nigeria.

Corresponding Author: [email protected] ; Tel: +234-8035995080
Recieved Date: January 10, 2019; Accepted Date: March 01, 2019; Published Date: 01 March 2019
Citation: Adesegun SA, Badejo MV, Odebunmi SO, Ojobo PD, H. B. Coker HB. Alpha Amylase Inhibitory and Antioxidant Activities of Leaf Extract and Fractions of Sorindea warneckei Engl. (Anacardiaceae). Trop J Nat Prod Res. 2019; 3(2):58-63. https://doi.org//10.26538/tjnpr/v3i2.7
Copyright: © 2019 Adesegun et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Postprandial hyperglycemia is characteristic of type 2 diabetes. The development of alternative medicines containing secondary metabolites with ability to delay or prevent glucose absorption will be a great therapeutic approach in the management of diabetes. The aim of the present study was to evaluate the in-vitro ?-amylase inhibitory and antioxidant activities of the leaf extract and fractions of Sorindea warneckei. The antidiabetic activity of the extract and its fractions were examined by ?-amylase bioassay. The antioxidant activity was measured using 2, 2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging and reducing activities. The HPLC analysis, total phenol and total flavonoid contents were also determined. The extract demonstrated substantial concentration-dependent ?-amylase inhibitory (IC50 = 400 µg/mL) and DPPH radical scavenging (IC50 = 40 µg/mL) activities. The ethyl acetate fraction showed the highest activity among the fractions with highest phenolic and flavonoid contents. Statistical analysis showed that total phenolic and flavonoid contents had significant (p < 0.05) effect on ?-amylase inhibitory and antioxidant activity. HPLC analysis revealed the presence of quercetin, rutin and gallic acid in the active ethyl acetate fraction. This study shows that ethanol leaf extract of Sorindea warneckei exhibits significant ?-amylase inhibitory and antioxidant activities which localize mainly in the ethyl acetate fraction. The plant is a veritable source of ?-amylase inhibitors.

Keywords: Sorindea warneckei, ?-amylase inhibition, antioxidant, phenols, flavonoids, HPLC analysis.
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